Navigation Links
Speed and power of X-ray laser helps unlock molecular mysteries
Date:7/26/2012

frozen molecules, however, prevents observation of the molecules at work in their native liquid environment at room temperature.

To obtain images of these molecules in the more natural state, the researchers sent the protein nanocrystals streaming in a single-file micron-sized droplet beam (rather like an ink-jet printer) in vacuum across the X-ray beam, in a method developed at Arizona State University.

Next they fired incredibly brief bursts of X-ray laser light, about 100 times each second, at the molecules in the droplet beam, and detected the scattered X-ray patterns from each particle before the intensity of the beam blasted them apart. The researchers were able to combine these millions of snapshots to build up 3D models of the molecules with atomic-scale resolution.

One particular molecule that was studied this way was Photosystem 1-ferredoxin, which is the chemical powerhouse that drives photosynthesis. The molecules for this experiment were made in the laboratory of Arizona State University researcher Petra Fromme.

Photosystem 1 harnesses sunlight to split water to make the oxygen we breathe, absorb carbon dioxide, and produce sugars, which maintains our biosphere. These molecules were studied "in action" by exciting them with a pulse of green laser light (to mimic the effect of sunlight falling on a leaf) a few microseconds before taking their X-ray snapshot. Each snapshot then became one frame of a movie. By changing the delay between green pulse and X-ray pulse, the researchers could create a 3D movie of a biomolecule in action.

"Many other groups we are supporting now are applying the method to other proteins, such as enzymes, drug molecule targets, and imaging chemical reactions as they develop along the liquid jet," said Spence. "The important thing was to get atomic-resolution snapshot images from nanocrystals at room temperature without radiation damage."


'/>"/>
Contact: Catherine Meyers
cmeyers@aip.org
301-209-3088
American Institute of Physics
Source:Eurekalert

Page: 1 2

Related biology news :

1. ECGs administered by paramedics can speed treatment for severe heart attacks
2. Rising carbon dioxide in atmosphere also speeds carbon loss from forest soils, IU-led research finds
3. Programmable RNA complex could speed genome editing in the lab
4. 7 pharmaceutical companies join academic researchers to speed TB drug discovery
5. Speeding up drug discovery with rapid 3-D mapping of proteins
6. Worlds largest release of comprehensive human cancer genome data helps speed discoveries
7. Analysis of speed of Greenland glaciers gives new insight for rising sea level
8. BGI and Aspera collaborate on high-speed data exchange to advance genome research
9. Need for speed
10. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
11. Powerful class of antioxidants may be potent Parkinsons treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/17/2014)... hydrogen sulfide in order to properly multiply and form ... Center for Craniofacial Molecular Biology at the Herman Ostrow ... principal investigator on the project, said the presence of ... of calcium ions. The essential ions activate a chain ... creation of new bone tissue, and keeps the breakdown ...
(Date:4/17/2014)... in German . ... money demanded of him, he can expect his premises to ... fear of the consequences is enough to make restaurant owners ... which lay their eggs in other birds, nests. If the ... take their revenge by destroying the entire nest. Consequently, it ...
(Date:4/17/2014)... France have hit on a novel method to ... correct and most effective treatment possible., Kidney stones ... and developing world. If left untreated, apart from ... failure and other complications. In many patients treated ... a more effective pathological approach to diagnosis and ...
Breaking Biology News(10 mins):Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3
... "dietary fibre". But what is dietary fibre and how do ... Structural Biology Laboratory, in collaboration with groups in Canada, the ... bacteria metabolise the complex dietary carbohydrates found in fruits and ... are about ten times more bacterial cells in the average ...
... alien world of aquatic micro-organisms just got new residents: ... has developed a class of tiny bio-hybrid machines that ... traverse the viscous fluids of biological environments on their ... Gutgsell Professor of mechanical science and engineering, the team ...
... researchers from the University of Nebraska-Lincoln, while using a ... Ice Shelf, unexpectedly discovered a new species of small ... tentacles protruding into frigid water like flowers from a ... it was really an amazing find," said Marymegan Daly, ...
Cached Biology News:York scientists investigate the fiber of our being 2Tiny swimming bio-bots boldly go where no bot has swum before 2New sea anemone species discovered in Antarctica 2New sea anemone species discovered in Antarctica 3New sea anemone species discovered in Antarctica 4
(Date:1/15/2014)... January 15, 2014 2013 was a ... Brain State Technologies®. They saw continued independent research led ... who were awarded a $1 million grant from the ... “Brain and Behavior” a peer reviewed journal, Amy Grant ...
(Date:1/14/2014)... 2014 Carahsoft and CDS Federal Services ... 2014 at 2pm EST (11am PST), “Natural Language Processing: ... on how technology can turn raw, heterogeneous data into ... agencies. The online webinar will last approximately one hour. ...
(Date:1/14/2014)... The largest international professional organization of scientists ... derivatives thereof has endorsed an educational program that ... challenges of adulterated herb and botanical products. ... The Society for Medicinal Plant and Natural Product ...
(Date:1/14/2014)... January 14, 2014 Global Record ... innovative information technology solutions for patients, physicians, the ... stakeholders, announced today the signing of a three-year ... and Drug Administration (FDA). This initiative is ...
Breaking Biology Technology:Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2
... N.J., Jan. 28 Arno Therapeutics, Inc., a,privately ... entered into,worldwide, exclusive license agreements with The Ohio ... novel, orally available,targeted therapies for cancer., Pursuant ... to several,small molecules for the treatment of cancer, ...
... Paper Provides Rationale for Continued Development of CNDO103 as ... ... Coronado Biosciences Inc. today,announced the publication of data demonstrating that the ... while,demonstrating less toxicity than Gossypol in vivo in mice., Published ...
... Jan. 28 /PRNewswire-FirstCall/ - ARIUS Research Inc., (TSX:,ARI), ... wave of,antibody therapeutics, today announced it has completed ... to discuss the upcoming IND,submission for ARH460-16-2, its ... anti-cancer antibody targeting a novel epitope of CD44,found ...
Cached Biology Technology:Arno Therapeutics Completes License Agreements for the Exclusive Rights to Novel, Targeted Cancer Compounds 2Arno Therapeutics Completes License Agreements for the Exclusive Rights to Novel, Targeted Cancer Compounds 3Study Shows Coronado Biosciences' Bcl-2 Inhibitor, Apogossypol, is More Efficacious, Less Toxic than Gossypol in Animal Models 2Study Shows Coronado Biosciences' Bcl-2 Inhibitor, Apogossypol, is More Efficacious, Less Toxic than Gossypol in Animal Models 3Arius completes Pre-IND Meeting with FDA for Lead CD44 Cancer Stem Cell Program 2Arius completes Pre-IND Meeting with FDA for Lead CD44 Cancer Stem Cell Program 3
... ejector is a unique product that facilitates ... their holder. Linkage: This CLS number is ... match Cornings product number. If showing no ... Sigma-Aldrich number (Z71,314-7) or contact customer service ...
Sigmas E-TOXATE (Limulus Amebocyte Lysate) test kits are intended for the detection and semiquantitation,of endotoxins for research purposes....
... modular cleanroom for biological safety labs ... cleanliness (to Class 10/ISO 3) and ... create ultra-smooth surfaces that are easy ... radius corners simplify cleaning. Unique double-wall ...
... The Staccato Series of systems provide ... drug discovery , genomics, proteomics and drug ... three base configurations; Mini Workstation Series, Application ... Sciclone ELISA Station is a remarkably compact ...
Biology Products: