Navigation Links
Speed and power of X-ray laser helps unlock molecular mysteries
Date:7/26/2012

frozen molecules, however, prevents observation of the molecules at work in their native liquid environment at room temperature.

To obtain images of these molecules in the more natural state, the researchers sent the protein nanocrystals streaming in a single-file micron-sized droplet beam (rather like an ink-jet printer) in vacuum across the X-ray beam, in a method developed at Arizona State University.

Next they fired incredibly brief bursts of X-ray laser light, about 100 times each second, at the molecules in the droplet beam, and detected the scattered X-ray patterns from each particle before the intensity of the beam blasted them apart. The researchers were able to combine these millions of snapshots to build up 3D models of the molecules with atomic-scale resolution.

One particular molecule that was studied this way was Photosystem 1-ferredoxin, which is the chemical powerhouse that drives photosynthesis. The molecules for this experiment were made in the laboratory of Arizona State University researcher Petra Fromme.

Photosystem 1 harnesses sunlight to split water to make the oxygen we breathe, absorb carbon dioxide, and produce sugars, which maintains our biosphere. These molecules were studied "in action" by exciting them with a pulse of green laser light (to mimic the effect of sunlight falling on a leaf) a few microseconds before taking their X-ray snapshot. Each snapshot then became one frame of a movie. By changing the delay between green pulse and X-ray pulse, the researchers could create a 3D movie of a biomolecule in action.

"Many other groups we are supporting now are applying the method to other proteins, such as enzymes, drug molecule targets, and imaging chemical reactions as they develop along the liquid jet," said Spence. "The important thing was to get atomic-resolution snapshot images from nanocrystals at room temperature without radiation damage."


'/>"/>
Contact: Catherine Meyers
cmeyers@aip.org
301-209-3088
American Institute of Physics
Source:Eurekalert

Page: 1 2

Related biology news :

1. ECGs administered by paramedics can speed treatment for severe heart attacks
2. Rising carbon dioxide in atmosphere also speeds carbon loss from forest soils, IU-led research finds
3. Programmable RNA complex could speed genome editing in the lab
4. 7 pharmaceutical companies join academic researchers to speed TB drug discovery
5. Speeding up drug discovery with rapid 3-D mapping of proteins
6. Worlds largest release of comprehensive human cancer genome data helps speed discoveries
7. Analysis of speed of Greenland glaciers gives new insight for rising sea level
8. BGI and Aspera collaborate on high-speed data exchange to advance genome research
9. Need for speed
10. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
11. Powerful class of antioxidants may be potent Parkinsons treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/23/2015)... , Feb. 23, 2015 NXT-ID, Inc. ... the "Company"), a biometric authentication company focused on the ... filed provisional patent 62114357 for DISTRIBUTED VOICE ... patent, NXT-ID introduces a new groundbreaking payment method. Payment ... payment account may only be accessed if both the ...
(Date:2/12/2015)... Feb. 12, 2015   MedNet Solutions , ... clinical study management systems, has recently bolstered its ... distinguishing iMedNet as the ... (CROs) and healthcare consultants.  Building on the Program,s ... referrals and numerous co-marketing opportunities), MedNet,s new and ...
(Date:2/9/2015)...  Lintec of America recently announced an exclusive license ... macrostructures, including sheets, yarns and ribbons, developed at the ... the vast industrial resources of the global Lintec Group, ... of America is forming the Nano-Science and Technology Center ... on scaling up the manufacturing and commercialization of nano ...
Breaking Biology News(10 mins):NXT-ID Patents Voice Directed Payments 2NXT-ID Patents Voice Directed Payments 3MedNet Solutions Announces Key Enhancements To Its iMedNet Partner Program 2Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 2Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 3Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 4
... CA February 4, 2013 A team led by ... cellular events that appear key to lupus, a debilitating autoimmune ... findings suggest that blocking this pathway in lupus-triggering cells could ... new study, described in an online Early Edition of the ...
... of activity at the back of the brain ... would respond to an experimental rapid-acting antidepressant, a ... "We have discovered a potential neuroimaging ... selection by revealing brain-based differences between patients," explained ...
... of American Societies for Experimental Biology (FASEB) announces ... Conference (SRC): TGF-β Superfamily: Signaling in Development and ... Development and Disease" is an important and unique ... family members in developmental and homeostatic processes is ...
Cached Biology News:Scientists find a key element of lupus, suggesting better drug targets 2Scientists find a key element of lupus, suggesting better drug targets 3Scientists find a key element of lupus, suggesting better drug targets 4Imaging biomarker predicts response to rapid antidepressant 2Imaging biomarker predicts response to rapid antidepressant 3
(Date:3/4/2015)... March 4, 2015 AcelRx Pharmaceuticals, Inc. ... company focused on the development and commercialization of ... breakthrough pain, today announced that it will release ... market close on Monday, March 9th, 2015. AcelRx ... 4:30 p.m. Eastern Time (1:30 p.m. Pacific Time) ...
(Date:3/4/2015)... 04, 2015 Follow us on ... of the fastest growing technologies in the life sciences ... visualization is driving growth in nucleic acid detection technologies ... of gene expressions will continue to benefit the qPCR ... fields ranging from pharma, biotech, food & beverage to ...
(Date:3/4/2015)... , March 4, 2015 ... Entwicklungen für Diagnostik und Therapie, Strahlendosismanagement und IT-Lösungen ... Royal Philips (NYSE: PHG, AEX: ... Röntgenkongress 2015 (ECR) bekannt, der vom 4. bis ... An den Ständen Nr. 102 and 110 ...
(Date:3/3/2015)... , March 3, 2015 NASA astronaut Scott ... this month to spend a year living and working on ... interviews from 5:30 to 7 a.m. EDT Monday, March 9. ... as he completes the final weeks of his training. ... video on NASA Television highlighting his mission training and previous ...
Breaking Biology Technology:AcelRx Pharmaceuticals to Hold Annual 2014 Financial Results Conference Call and Webcast on Monday, March 9th, 2015 2AcelRx Pharmaceuticals to Hold Annual 2014 Financial Results Conference Call and Webcast on Monday, March 9th, 2015 3Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 2Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 3Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 4Philips stellt integrierte Bildgebungslösungen auf dem Europäischen Röntgenkongress 2015 vor 2Philips stellt integrierte Bildgebungslösungen auf dem Europäischen Röntgenkongress 2015 vor 3Philips stellt integrierte Bildgebungslösungen auf dem Europäischen Röntgenkongress 2015 vor 4Philips stellt integrierte Bildgebungslösungen auf dem Europäischen Röntgenkongress 2015 vor 5Philips stellt integrierte Bildgebungslösungen auf dem Europäischen Röntgenkongress 2015 vor 6NASA Astronaut Scott Kelly Available for Interviews before One-Year Space Station Mission 2
... KNDL ), a,leading, global full-service clinical research ... Financial Officer Karl,"Buzz" Brenkert, III will participate in ... Grand Hyatt New York, Park Avenue at Grand,Central, ... is,scheduled for Tuesday, Sept. 11 at 4:30 p.m. ...
... (ET), SAN DIEGO, Sept. 7 NuVasive, Inc. ... for,minimally disruptive surgical treatments for the spine, announced today,that ... and,analysts at the Waldorf=Astoria Hotel in New York City ... p.m. ET. The reception will be hosted by ...
... - ARIUS Research Inc., (TSX:,ARI), a biotechnology company ... today announced that it will deliver poster,presentations at ... its anti-cancer antibody drug candidates. At the ... September 7-8,Arius will report recent results of its ...
Cached Biology Technology:Kendle to Present at Bear Stearns 20th Annual Healthcare Conference 2NuVasive to Host Investor Reception on September 11, 2007 2NuVasive to Host Investor Reception on September 11, 2007 3NuVasive to Host Investor Reception on September 11, 2007 4Arius Features Preclinical Data on Antibody Candidates at Two Conferences 2Arius Features Preclinical Data on Antibody Candidates at Two Conferences 3
... supplies, for 10 chips, provides the ... standard-sensitivity RNA analysis (nanogram levels) with ... electrophoresis system. Supplied are 1,250 microliters ... stain, 20 microliters RNA ladder, 900 ...
... for enhanced translation of cloned sequences in vitro ... The maps for pCITE-2b(+) and pCITE-2c(+) are the ... is a 3793 bp plasmid; it lacks Nde ... sites. Subtract 7 bp from all sites beyond ...
MAb to Verotoxin II (SLT-2a) Verotoxin II (Shiga-like) A subunit (SLT-2a)...
Anti-NCAPD3 Monoclonal AntibodyOther Sub-Family: not assigned-Other Applications: Immunoprecipitation, Western Blot...
Biology Products: