Navigation Links
Speed and power of X-ray laser helps unlock molecular mysteries
Date:7/26/2012

By outrunning a laser's path of destruction, an international research team has created 3D images of fragile but biologically important molecules inside protein nanocrystals. Using the Linac Coherence Light Source (LCLS), a powerful X-ray laser at the SLAC National Accelerator Laboratory in Menlo Park, Calif., the scientists fired femtosecond (one quadrillionth of a second) bursts of light at a stream of tumbling molecules, obliterating them as they pass, but not before capturing otherwise illusive images of their crystalline structures.

An overview and early results of this new imaging technique will be presented at the 2012 meeting of the American Crystallographic Association (ACA), which takes place July 28 Aug. 1 in Boston, Mass.

"These laser pulses are so brief that we are able to outrun the radiation's damaging effects," said John C.H. Spence of Arizona State University, one of more than 70 international researchers from institutions including SLAC; DESY, the German Electron Synchrotron; and the Max-Planck Institute in Heidelberg, Germany.

"Using this so-called 'diffract-then-destroy' approach, our research team recorded about a hundred scattering patterns per second from protein nanocrystals," said Spence. "This is an important step toward the making of movies of biomolecules at work."

In traditional crystallography, a beam of X-rays first interacts with a crystal and then appears on a photo-detector as diffraction spots of greater and lesser intensity. These patterns encode the density of electrons in the crystal, enabling scientists to determine the three-dimensional position of atoms, chemical bonds, and other information. To obtain this information, the crystal is frozen, to reduce radiation damage, and placed on a rotating mount and bombarded with X-rays as its orientation is changed. A scattering pattern is slowly built up and the 3D structure can eventually be deduced.

This traditional method of using frozen molecules, however, prevents observation of the molecules at work in their native liquid environment at room temperature.

To obtain images of these molecules in the more natural state, the researchers sent the protein nanocrystals streaming in a single-file micron-sized droplet beam (rather like an ink-jet printer) in vacuum across the X-ray beam, in a method developed at Arizona State University.

Next they fired incredibly brief bursts of X-ray laser light, about 100 times each second, at the molecules in the droplet beam, and detected the scattered X-ray patterns from each particle before the intensity of the beam blasted them apart. The researchers were able to combine these millions of snapshots to build up 3D models of the molecules with atomic-scale resolution.

One particular molecule that was studied this way was Photosystem 1-ferredoxin, which is the chemical powerhouse that drives photosynthesis. The molecules for this experiment were made in the laboratory of Arizona State University researcher Petra Fromme.

Photosystem 1 harnesses sunlight to split water to make the oxygen we breathe, absorb carbon dioxide, and produce sugars, which maintains our biosphere. These molecules were studied "in action" by exciting them with a pulse of green laser light (to mimic the effect of sunlight falling on a leaf) a few microseconds before taking their X-ray snapshot. Each snapshot then became one frame of a movie. By changing the delay between green pulse and X-ray pulse, the researchers could create a 3D movie of a biomolecule in action.

"Many other groups we are supporting now are applying the method to other proteins, such as enzymes, drug molecule targets, and imaging chemical reactions as they develop along the liquid jet," said Spence. "The important thing was to get atomic-resolution snapshot images from nanocrystals at room temperature without radiation damage."


'/>"/>
Contact: Catherine Meyers
cmeyers@aip.org
301-209-3088
American Institute of Physics
Source:Eurekalert

Related biology news :

1. ECGs administered by paramedics can speed treatment for severe heart attacks
2. Rising carbon dioxide in atmosphere also speeds carbon loss from forest soils, IU-led research finds
3. Programmable RNA complex could speed genome editing in the lab
4. 7 pharmaceutical companies join academic researchers to speed TB drug discovery
5. Speeding up drug discovery with rapid 3-D mapping of proteins
6. Worlds largest release of comprehensive human cancer genome data helps speed discoveries
7. Analysis of speed of Greenland glaciers gives new insight for rising sea level
8. BGI and Aspera collaborate on high-speed data exchange to advance genome research
9. Need for speed
10. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
11. Powerful class of antioxidants may be potent Parkinsons treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/22/2016)... Ontario , PROVO and ... Newborn Screening Ontario (NSO), which operates the ... for molecular testing, and Tute Genomics and UNIConnect, ... management technology respectively, today announced the launch of a ... next-generation sequencing (NGS) testing panel. NSO ...
(Date:3/15/2016)... 15, 2016 --> ... Transparency Market Research "Digital Door Lock Systems Market - Global ... 2023," the global digital door lock systems market in terms ... and is forecast to grow at a CAGR of 31.8% ... and medium enterprises (MSMEs) across the world and high industrial ...
(Date:3/11/2016)... 2016 http://www.apimages.com ) - --> ... available at AP Images ( http://www.apimages.com ) - ... to produce the new refugee identity cards. DERMALOG will be unveiling ... in Hanover next week.   --> ... used to produce the new refugee identity cards. DERMALOG will be ...
Breaking Biology News(10 mins):
(Date:5/24/2016)... ... May 24, 2016 , ... Media Cybernetics, global ... Media Cybernetics corporate branding reflects a results-driven revitalization for a company with a ... re-branding components include a crisp, refreshed logo and a new web presence. , ...
(Date:5/23/2016)... LONDON , May 23, 2016 ... Boost Efficiency by 40% - Frontage Implement a Single ... Enforce Quality, Compliance and Traceability Within the Bioanalytical lab ... the United States and China ... deployed across its laboratory facilities. In addition to serving as the ...
(Date:5/20/2016)... ... ... Kablooe Design, a leading provider of product design and development services to medical ... “We have worked hard to build long-term relationships,” says President and CEO, Tom KraMer. ... serving their product design and development needs through the years.” , Kablooe has earned ...
(Date:5/19/2016)... , May 19, 2016 ... will fully recover given the relentless pressures in pricing ... sure in the investors circle though - numerous opportunities ... Ahead of today,s session, ActiveWallSt.com,s presents four names in ... ), Vitae Pharmaceuticals Inc. (NASDAQ: VTAE ), ...
Breaking Biology Technology: