Navigation Links
Solving the puzzle of the BK ion channel
Date:6/23/2010

e pore.

What about the ligand gating? When calcium binds to the cystolic domain, it changes the cystolic domain's shape (its conformation), and the domain tugs on another linker chain connected to one of the pore-lining helices as it warps.

The channel's dynamics

In trying to clarify the effect of the mutation on BK channel function, Cui's group uncovered yet another mechanism for action at a distance.

After the mutation's discovery, experiments showed that it affects the pathway between a calcium-binding site on the cystolic domain called D367 and the channel's pore. The epilepsy-causing mutation is very close to the binding site but not part of it.

So how was the mutation making it easier to open the channel?

Cui wondered if it didn't have something to do with the mechanical properties of the amino acid that is substituted into the mutated form of the channel. The amino acid is glycine, which is exceptionally flexible; the presence of gylcine can allow a protein to bend at that position like a mechanical joint.

The scientists' idea was that this local flexibility might actually make the pathway between the D367 binding site and the pore more rigid, which in turn might make the channel more sensitive to calcium binding.

To see whether dynamics mattered, Cui's team immersed wild type channels in thick solutions of sugar or glycerol. The viscous solutions, which mimicked a more rigid D367 pathway, did indeed increase the channel's sensitivity to calcium.

To explore further, Cui's team then simulated the D367 pathway and its dynamics. The simulation showed that the wild-type protein is more flexible than the mutated protein and that glycine did indeed reduce the flexibility of other areas of the pathway.

The effect of the mutation, Cui concluded, was to make the D367 pathway move as a more rigid entity that couples the calcium binding site more tightly to the pore, so that it w
'/>"/>

Contact: Diana Lutz
dlutz@wustl.edu
314-935-5272
Washington University in St. Louis
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Brains problem-solving function at work when we daydream
2. Bowman Global Change says public engagement critical to solving climate crisis
3. Fossils and layers: Solving the terrane puzzle of the North American cordillera
4. Solving an avian scourge could also provide benefits to human health
5. New tool for resolving fisheries conflicts
6. Magnetic snakes control fluids, gravity-defying droplets, and solving a dragonfly mystery
7. McGill team discovers a piece of the puzzle for individualized cancer therapy via gene silencing
8. Key piece of puzzle sheds light on function of ribosomes
9. CSHL scientists harness logic of Sudoku math puzzle to vastly enhance genome-sequencing capability
10. New piece found in the puzzle of epigenetics
11. Biologists devise unifying framework to explain evolutionary puzzles
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Solving the puzzle of the BK ion channel
(Date:10/18/2014)... patients referred for evaluation of suspected genetic conditions, ... 25 percent, including detection of a number of ... disease, according to a study appearing in ... coincide with the American Society of Human Genetics ... or coding regions of thousands of genes simultaneously ...
(Date:10/17/2014)... Researchers from the University of Copenhagen have shown for ... the lungs of Cystic fibrosis patients, giving them the ... growth in chronic infections. , The study also discovered ... fibrosis (CF) patients was halted or slowed down by ... consumed all the oxygen and helped "suffocate" the bacteria, ...
(Date:10/16/2014)... Oregon and Washington land managers have a new synthesis ... eastside moist mixed-conifer (MMC) forests in the two states. ... Forests in Eastern Oregon and Washington: A Synthesis of ... Management, a general technical report published by the U.S. ... response to a request from managers for a synthesis ...
Breaking Biology News(10 mins):Whole-exome sequencing shows potential as diagnostic tool 2Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2New report synthesizes best available science on management of moist mixed-conifer forests 2
... at the Smithsonian and partnering organizations have discovered a remarkably ... the Republic of Palau. This fish exhibits many primitive anatomical ... 800 species of living eels, resulting in its classification as ... The team,s research is published online in the Proceedings ...
... Sons of male mice exposed to prenatal stress are ... in the August 17 issue of The Journal of ... lead to individual differences in stress response that may be ... of Pennsylvania, and colleagues previously found that male mice were ...
... and Switzerland have investigated the remarkable distance that microorganisms ... about their potential to colonise new lands and also ... John Moores University (LJMU), Swiss Federal Institute for Forest, ... Fdrale de Lausanne (EPFL) the University of Neuchtel published ...
Cached Biology News:Scientists discover the most primitive living eel 2Effects of prenatal stress passed across generations in mice 2Life on the wind: Study reveals how microbes travel the Earth 2
(Date:10/20/2014)... 2014 OncLive® is ... Center at Thomas Jefferson University has joined its ... Strategic Alliance Partnership program, the Sidney Kimmel Cancer ... raise awareness of the Center’s cutting-edge research programs, ... Clinicians and other health care professionals from the ...
(Date:10/19/2014)... 2014 OCTOBER 20-22, 2014: ... ABIM will take place at the ... ABIM 2014 is now available at ... companies and organizations from all over the ... on the latest products and developments on ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The Asia-Pacific ... Analytics market in Asia-Pacific with analysis and forecast of ... million in 2014 to $208 million by 2019, at ... 2014 to 2019. , Browse through the TOC of ... idea of the in-depth analysis provided. It also provides ...
(Date:10/19/2014)... 2014 According to market research ... WLAN Controllers; Wireless gateways)]- Global Advancements, Forecasts and ... global Outdoor Wi-Fi market with analysis and forecasting ... also identifies the drivers and the restrains for ... opportunities and challenges. , Browse 65 market data ...
Breaking Biology Technology:Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4
... Informex , the leading meeting place for buyers ... the Global Pharma Sourcing Conference to be held December ... Philadelphia, Pennsylvania. The two day program will be jointly ... address key issues that global chemical and pharmaceutical industry ...
... Corp. (IDC), a privately held biotechnology company developing ... and allergy, announced today that it has entered ... investigate the use of IDC,s Glucopyranosyl Lipid Adjuvant ... in the field of allergy. (Logo: ...
... Inc. (Nasdaq: SGMO ) today reported third quarter ... third quarter ended September 30, 2011, Sangamo reported a consolidated ... to a net loss of $8.7 million, or $0.19 per ... 30, 2011, the Company had cash, cash equivalents and marketable ...
Cached Biology Technology:Informex Introduces a Thorough Global Pharma Perspectives Conference 2Sangamo BioSciences Reports Third Quarter 2011 Financial Results 2Sangamo BioSciences Reports Third Quarter 2011 Financial Results 3Sangamo BioSciences Reports Third Quarter 2011 Financial Results 4Sangamo BioSciences Reports Third Quarter 2011 Financial Results 5Sangamo BioSciences Reports Third Quarter 2011 Financial Results 6
Although each Beacon 2000 System includes one preinstalled sample chamber (size specified at time of order), optional chambers are available and easily retrofit by the user....
Whole blood is collected from healthy guinea pigs of either sex from US source which are free of visible diseases at time of blood collection. Hemolytic titer: CH50 = 435 units...
...
... serial preparation of four ... cytosol/particulate/cytoskeleton/nuclear fractions from one ... can directly be used ... as 2-D gel/Western blot/gel-shift/translocation/enzyme ...
Biology Products: