Navigation Links
So many proteins, so much promise
Date:10/30/2011

The human genome has been mapped. Now, it's on to proteins, a much more daunting task. There are 20,300 genes, but there are millions of distinct protein molecules in our bodies. Many of these hold keys to understanding disease and targeting treatment.

A team led by Northwestern University chemical biologist Neil Kelleher has developed a new "top-down" method that can separate and identify thousands of protein molecules quickly. Many have been skeptical that such an approach, where each protein is analyzed intact instead of in smaller parts, could be done on such a large scale.

The promise of a top-down strategy is that the molecular data scientists do collect will be more closely linked to disease.

"Accurate identification of proteins could lead to the identification of biomarkers and early detection of disease as well as the ability to track the outcome of treatment," Kelleher said. "We are dramatically changing the strategy for understanding protein molecules at the most basic level. This is necessary for the Human Proteome Project -- the mapping of all healthy human proteins in tissues and organs -- to really take off."

Kelleher is the Walter and Mary E. Glass Professor of Molecular Biosciences and professor of chemistry in the Weinberg College of Arts and Sciences. He also is director of the Proteomics Center of Excellence and a member of the Robert H. Lurie Comprehensive Cancer Center of Northwestern University.

Kelleher says his approach is conceptually simple. "We take proteins -- those swimming around in cells -- and we measure them," he said. "We weigh proteins precisely and identify them directly. The way everyone else is doing it is by digesting the proteins, cutting them up into smaller bits called peptides, and putting them back together again. I call it the Humpty Dumpty problem."

The new strategy, Kelleher says, solves the "protein isoform problem" of the "bottom-up" approach where the smalle
'/>"/>

Contact: Megan Fellman
fellman@northwestern.edu
847-491-3115
Northwestern University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Linking Proteins, Wires, Dots, and Molecules into Useful Devices
2. Neural tissue contains imbalanced levels of proteins, U-M study finds
3. Proteins, like people, act differently when crowded together
4. New approach to treating cystic fibrosis lung infection shows promise
5. More research needed to make good on biofuel promise, experts say
6. Text focuses on diagnosing infections in immunocompromised patients
7. Research identifies type of vaccine that holds promise in protecting against TB
8. New test promises quicker, more accurate evaluation for cystic fibrosis patients
9. Oral rinses used for tracking HPV-positive head and neck cancers holds promise for cancer screening
10. JDRF funded research shows promise for prevention, reversal of type 1 diabetes
11. Two new compounds show promise for eliminating breast cancer tumors
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/22/2015)... 15, 2015  BellBrook Labs, a leader in high ... launch of a TR-FRET (time resolved Forster resonance ... Assay, a high throughput screening assay for glycosyltransferases ... allow for sensitive detection of hundreds of human ...
(Date:1/22/2015)... MUNICH , January 22, 2015 ... its tenth year  The European Patent Office to present ... Two British nominations to be featured: Christofer Toumazou ... week featuring former finalists and winners of the Award   ...
(Date:12/22/2014)... , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: NXTD ... company focused on the growing mobile commerce market, announces it ... Robbins for the 2015 International CES debut of the ... will be at the NXT-ID booth January 6th and ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4
... a pointer point, a sheep dog herd, and a retriever ... Danes? And how can a tiny Chihuahua possibly be ... in size, shape, color, coat length and behavior more than ... been unexplained. Now, scientists have developed a method to identify ...
... Smithsonian,s National Zoo detected a secondary rise in urinary ... Xiang (may-SHONG) earlier this month. The results from the ... the hormone rise indicates that it would be mid- ... a cub or comes to the end of a ...
... ARGONNE, Ill. (June 20, 2008) The future of ... scientists at U.S. Department of Energy,s Argonne National Laboratory ... sources. "The free electron laser oscillator (X-FELO) we ... million times brighter than currently operating machines," Argonne Distinguished ...
Cached Biology News:DNA study unlocks mystery to diverse traits in dogs 2A novel X-ray source could be brightest in the world 2
(Date:1/22/2015)... January 22, 2015 Dr. Greg Leyer of ... annual Scripps Natural Supplements Pre-Conference seminar on probiotics in San ... an annual continuing education conference for health care professionals. This ... the topic of probiotics in health. Dr. Leyer spoke about ...
(Date:1/22/2015)... 22, 2015 Selexis SA, a serial ... Cell Banks (RCBs) used for drug discovery to commercial ... will include Next-Generation Sequencing (NGS) data packages. ... biologic manufacturing by ensuring the integrity of the gene, ...
(Date:1/22/2015)... , 22 de enero de 2015  El Dr. ... su llamada a nominaciones para 2015. Este prestigioso premio ... o tiene el potencial para hacer, contribuciones destacadas para ... aceptarán hasta el 15 de marzo de 2015 a ...
(Date:1/22/2015)...  Varian Medical Systems (NYSE: VAR ), world leader ... commitment to sustainability with inclusion on a prestigious list of ... healthcare equipment company among the Corporate Knights Global 100 Most ... at Davos, Switzerland . ...
Breaking Biology Technology:Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Varian Honored Among World's 100 Most Sustainable Corporations 2
... Larissa Tan, Bio-Rad Laboratories, Inc.,Hercules CA 94547 USA , ... General Information , The ... in molecular biology. The use of this technique has generated unprecedented ... expression analysis. , In addition, new techniques that ...
... , Larissa Tan, Marni Brisson, Robert Park, and Keith ... Hercules, CA 94547 , USA , ... , The detection of fluorescence resonance ... reaction (PCR) and melt-curve analysis is a , ...
... Bowes Rickman 1, 2, 1 Departments of , ... NC 27710 USA , Correspondence: Catherine ... Medical Center, Box 3802, Durham, NC 27710, Phone (919) , ... Introduction , ...
Cached Biology Technology:Real-Time PCR: General Considerations, Rev A 2Real-Time PCR: General Considerations, Rev A 3Real-Time PCR: General Considerations, Rev A 4Real-Time PCR: General Considerations, Rev A 5Real-Time PCR: General Considerations, Rev A 6Real-Time PCR: General Considerations, Rev A 7Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 2Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 3Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 4Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 5Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 6Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 7Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 2Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 3Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 4Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 5Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 6Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 7Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 8
... The Schleicher & Schuell BioScience ... center dedicated to custom protein and ... experienced project teams, comprised of R&D, ... work closely with collaborators every step ...
... acid synthetic peptide whose sequences are derived from mouse resistin protein. ... S(21) - S - M - P - L - C ... I - D - K - K - I - K ... be used for neutralization and control experiments with the polyclonal antibody ...
... Molecular Imaging (MI) Software for use in ... and drug discovery applications. The Regulatory Edition ... Drug Administrations Code of Federal Regulations Title ... [21 CFR Part 11]. KODAK MI Software, ...
... for pathway studies, protein-protein interaction studies, ... built on the flexible and powerful ... microarrays are available as part of ... Custom Probe Content ...
Biology Products: