Navigation Links
Small nanoparticles bring big improvement to medical imaging
Date:11/18/2009

If you're watching the complex processes in a living cell, it is easy to miss something importantespecially if you are watching changes that take a long time to unfold and require high-spatial-resolution imaging. But new research* makes it possible to scrutinize activities that occur over hours or even days inside cells, potentially solving many of the mysteries associated with molecular-scale events occurring in these tiny living things.

A joint research team, working at the National Institute of Standards and Technology (NIST) and the National Institute of Allergy and Infectious Diseases (NIAID), has discovered a method of using nanoparticles to illuminate the cellular interior to reveal these slow processes. Nanoparticles, thousands of times smaller than a cell, have a variety of applications. One type of nanoparticle called a quantum dot glows when exposed to light. These semiconductor particles can be coated with organic materials, which are tailored to be attracted to specific proteins within the part of a cell a scientist wishes to examine.

"Quantum dots last longer than many organic dyes and fluorescent proteins that we previously used to illuminate the interiors of cells," says biophysicist Jeeseong Hwang, who led the team on the NIST side. "They also have the advantage of monitoring changes in cellular processes while most high-resolution techniques like electron microscopy only provide images of cellular processes frozen at one moment. Using quantum dots, we can now elucidate cellular processes involving the dynamic motions of proteins."

For their recent study, the team focused primarily on characterizing quantum dot properties, contrasting them with other imaging techniques. In one example, they employed quantum dots designed to target a specific type of human red blood cell protein that forms part of a network structure in the cell's inner membrane. When these proteins cluster together in a healthy cell, the network provides mechanical flexibility to the cell so it can squeeze through narrow capillaries and other tight spaces. But when the cell gets infected with the malaria parasite, the structure of the network protein changes.

"Because the clustering mechanism is not well understood, we decided to examine it with the dots," says NIAID biophysist Fuyuki Tokumasu. "We thought if we could develop a technique to visualize the clustering, we could learn something about the progress of a malaria infection, which has several distinct developmental stages."

The team's efforts revealed that as the membrane proteins bunch up, the quantum dots attached to them are induced to cluster themselves and glow more brightly, permitting scientists to watch as the clustering of proteins progresses. More broadly, the team found that when quantum dots attach themselves to other nanomaterials, the dots' optical properties change in unique ways in each case. They also found evidence that quantum dot optical properties are altered as the nanoscale environment changes, offering greater possibility of using quantum dots to sense the local biochemical environment inside cells.

"Some concerns remain over toxicity and other properties," Hwang says, "but altogether, our findings indicate that quantum dots could be a valuable tool to investigate dynamic cellular processes."


'/>"/>

Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Related biology news :

1. Small, beautiful and additive-free
2. Small mechanical forces have big impact on embryonic stem cells
3. Fate Therapeutics announces creation of small molecule platform for commercial-scale reprogramming
4. Conservation targets too small to stop extinction
5. Small molecule inhibits pathology associated with myotonic dystrophy type 1
6. Small rodents encourage the formation of scrubland in Spain
7. Harvard research team receives $10M NSF grant to develop small-scale mobile robotic devices
8. After dinosaurs, mammals rise but their genomes get smaller
9. Smaller than expected, but severe, dead zone in Gulf of Mexico
10. Smaller plants punch above their weight in the forest, say Queens biologists
11. New research shows dinosaurs may have been smaller than we thought
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Small nanoparticles bring big improvement to medical imaging
(Date:2/2/2017)... Feb. 1, 2017  Central to its deep ... advances worldwide, The Japan Prize Foundation today announced ... have pushed the envelope in their respective fields ... Three scientists are being recognized with the 2017 ... not only contribute to the advancement of science ...
(Date:1/30/2017)... FRANCISCO , Jan. 30, 2017   Invitae ... the fastest growing genetic information companies, today announced that ... financial results and provide 2017 guidance on Monday, February ... conference call that day at 4:45 p.m. Eastern / ... Invitae,s management team will briefly review financial results, guidance, ...
(Date:1/25/2017)... , Jan. 25, 2017 The Elements ... Management (IAM) lifecycle is comprised of a comprehensive ... the purpose of maintaining digital identities and providing ... and applications. There are significant number of programs ... time to time by optimizing processes and changing ...
Breaking Biology News(10 mins):
(Date:2/20/2017)... San Francisco, CA (PRWEB) , ... February 20, ... ... to reduce infections in patients with catheters associated with peritoneal dialysis, announced today ... (UV) light-based peritoneal dialysis catheter connection system in Peritoneal Dialysis International (PDI), ...
(Date:2/20/2017)... -- Atrius Health and IBM (NYSE: IBM ) ... develop a cloud based service designed to improve ... of the multiple influences on an individual,s health, ... designed to support shared decision making between physicians ... healthcare organization with 875 physicians caring for 675,000 ...
(Date:2/18/2017)... ... February 17, 2017 , ... The BMT Tandem Meetings of ... International Blood & Marrow Transplant Research (CIBMTR) will take place Feb. 22-26, 2017 ... combined scientific sessions offer investigators, clinicians, laboratory technicians, clinical research professionals, nurses, pharmacists, ...
(Date:2/17/2017)... PUNE, India , February 17, 2017 /PRNewswire/ ... research report "Biomarkers Market by Product (Consumables, Service), ... Disorders), Application (Diagnostics Development, Drug Discovery and Development, ... MarketsandMarkets, the market is projected to reach USD ... in 2016, growing at a CAGR of 13.8% ...
Breaking Biology Technology: