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Small, beautiful and additive-free
Date:11/13/2009

The National Research Council Canada (NRC) recently helped Olympus, a world leader in advanced optical microscopy and medical imaging, to design and commercialize a CARS (Coherent Anti-stokes Raman Scattering) microscope. A new CARS user facility will open its doors to Canadian researchers and the medical community in Ottawa on November 17, 2009.

The new Olympus microscope is based on a femtosecond non-linear optical process called CARS, which stands for Coherent Anti-Stokes Raman Scattering. CARS is a molecule-specific imaging method that permits researchers to see particular chemicals and structures inside living cells, without adding any dyes or stains: CARS is additive-free! While CARS itself is not new, the NRC team developed a simple, robust yet high performance approach that permitted rapid commercialization. CARS microscopy will now be available to biological and medical researchers in a cost-effective system. New opportunities will abound.

Cells are very small and optical microscopes are required to see them. Unfortunately, the internal components of cells do not come colour-coded for our convenience. To deal with this, scientists have developed, over many decades, various dyes and stains to colour the internal bits of cells so that they could be discerned in an optical microscope. However, if one wants to study processes in live cells busily going about their business, there is a problem. Cellular processes and biochemistry are exceedingly complex and no one knows for sure if these added dyes or stains alter any of these biochemical processes. The best, of course, would be to observe live cells without adding anything at all. Enter CARS microscopy.

CARS is based upon the internal vibrations of molecules. Different molecules have different structures. Due to this, the internal vibrations of these molecules will also be different. CARS recognizes molecules by their "vibrational fingerprint". It is this unique characteristic t
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Contact: Roxanne DeAbreu-Breen
Roxanne.DeAbreu@olympus.com
905-969-3201
National Research Council of Canada
Source:Eurekalert

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