Navigation Links
SlipChip counts molecules with chemistry and a cell phone
Date:11/18/2013

nology: the smartphone.

The researchers placed the SlipChip in a makeshift darkroom (a shoebox with a hole in the top) and then photographed its wells using a smartphone outfitted with a special filter attachmentso that the smartphone flash would be able to "excite" the fluorescent DNA dye, and the smartphone camera could capture an image of the fluorescence. The resulting images were uploaded to Microsoft SkyDrive, a cloud-based server, where custom softwaredesigned by the researchersdetermined the viral load concentration and sent the results back in an email. These capabilities allow the digital approach to perform reliably with automated processing, regardless of how poor the imaging conditions may be. As an example of its simplicity, a 5-year-old child was able to use this cell phone imaging method to obtain quantitative results using strands of RNA extracted from a noninfectious virus (a video of this demonstration is available on the Ismagilov lab's YouTube channel).

"We were surprised that this cell phone method worked, because both cell phone imaging and automated processing are error prone," Ismagilov says. "Because digital assays involve simply distinguishing positives from negatives, we found that even these error-prone approaches can be used to count single molecules reliably."

The fact that this method is robust not only to changes in time and temperature but also is amenable to cell phone imaging and automated processing makes it a promising technology for limited-resource settings. "We believe that our findings of the robustness of digital amplification could signal a major paradigm shift in how quantitative measurements are obtained at home, in the field, and in developing countries," Ismagilov says.

The researchers stress that there is still room for improvement, however. "While in this study we were examining robustness and used purified RNA, the next generation of devices will isolate HIV RNA molecules directl
'/>"/>

Contact: Deborah Williams-Hedges
mr@caltech.edu
626-395-3227
California Institute of Technology
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. EyeLock Appoints Michael Fiorito Vice President of Enterprise Accounts
2. Quantum communication: Each photon counts
3. Global Biotech and Pharmaceutical Conference Early Bird Registration Discounts End This Week
4. Early Bird Discounts Available on International Healthcare and Biotechnology Conferences, Save Up To $600 with Global Information
5. Attendees Save Up To $800 on Boston-area Pharmaceutical and Healthcare Conferences, Early Bird Discounts Expiring April 27
6. Lawrence Livermore researchers unveil carbon nanotube jungles to better detect molecules
7. Biomolecules for the production line
8. CCNY chemists devise new way to prepare molecules for drug testing
9. Facebook for molecules
10. Detecting mirror molecules
11. Natural keystone molecules punch over their weight in ecosystems
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/12/2015)... Aug. 12, 2015   MedNet Solutions , an ... spectrum of clinical research, is proud to announce that ... comprehensive SaaS-based eClinical technology platform, has led the way ... two quarters of 2015.   Q2 2015 and Q1 ... measured by contract value sold in the company,s 15 ...
(Date:8/11/2015)... August 11, 2015 Today, ZUK announced its ... as well as expected revenues in 2015 that relate to sales ... revenue guidance of approximately 2,200 MSEK for 2015. Jörgen ... prominent smartphone manufacturer in China ... selected FPC1 155 for Z1 ...
(Date:8/6/2015)... BOSTON and TELTOW, Germany ... the 2015 Siggraph Conference, SensoMotoric Instruments (SMI) ... augmented reality , based on Epson,s Moverio ... eye tracking platform. With this new solution, unprecedented quality ... multi-modal, hands-free interaction with context-sensitive displays. For the first ...
Breaking Biology News(10 mins):MedNet Solutions Experiences Explosive Corporate Growth 2FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3
... forecasting models are useful tools to help individuals and ... at risk from forest fire smoke, according to University ... shares his insight into the health impacts of smoke ... at the American Association for the Advancement of Science ...
... about neuroscience? How does melting permafrost impact the citizens ... cause energy use to spike and threaten Canada,s water ... and many other questions through presentations at the American ... the Vancouver Conference Centre Feb. 16 to 20 in ...
... Feb. 17, 2012  ChromaDex Corporation (OTCBB: CDXC), an ... solutions and ingredients to the dietary supplement, food ... of BluScience™ ( www.bluscience.com ), its ... that William F. Spengler has elected to step ...
Cached Biology News:As climate change increases forest fires, smoke forecasting could help protect public health 2UVic researchers among presenters at global science conference 2ChromaDex® Announces Management Changes 2ChromaDex® Announces Management Changes 3
(Date:8/28/2015)... Pittsburgh, PA (PRWEB) , ... August 28, 2015 ... ... Program Chairman, Chuck Gardner, has organized and will present a session on chemical ... September 2. This conference and exposition is Asia’s largest analytical and scientific instruments ...
(Date:8/27/2015)... ... August 27, 2015 , ... The Kansas ... investment firms and animal health companies to hear from animal health companies with ... have also received licensing agreements or distribution contracts. This meeting is one ...
(Date:8/27/2015)... Aug. 27, 2015 The National Necrotizing ... eyes—and life—were saved by doctors at Vanderbilt University Hospital ... NNFF, NovaBay Pharmaceuticals and Dr. John Crew , ... at Seton Medical Center in Daly City, ... a breakthrough approach, pioneered by Dr. Crew, that had ...
(Date:8/27/2015)... -- GlassesOff (OTCBB: GLSO), a visual neuroscience technology ... vision system, announced today a partnership with eight-time All-Star, ... to develop a new mobile app designed for professional ... on-court performance. Vision is the inception of every ... ball or blocking a pass. The critical impact vision speed ...
Breaking Biology Technology:U.S. Symposium on Imaging Organized by Pittcon’s Program Chairman Chuck Gardner 2U.S. Symposium on Imaging Organized by Pittcon’s Program Chairman Chuck Gardner 3VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 2VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 3Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 3
... Fazil Madison, Wis. - The 2006 winner ... has launched its first identity theft product, and has an ... three million customers after two years. , ,This probably sounds ... to reach positive cash flow, but consider the total market ...
... Wis. - Two more men have pleaded guilty ... a result of their selling nearly $6 million in ... eBay . , ,Assistant Attorney General Alice Fisher for ... the Eastern District of Wisconsin announced that ...
... - The United States Department of Energy ... three new Bioenergy Research Centers, including $125 million for a ... promote the development of cellulosic ethanol. , ,The Madison ... Great Lakes Bioenergy Research Center, will operate in collaboration with ...
Cached Biology Technology:Identity theft start up has fast-track growth plans 2Identity theft start up has fast-track growth plans 3Two convicted of selling $6 million in counterfeit Rockwell software on eBay 2UW-Madison to receive $125M for bioenergy research center 2UW-Madison to receive $125M for bioenergy research center 3
Template and primers for preparation of internal control spots...
Cross reactivity to St. Louis encephalitis (MSI-7) is determined by ELISA....
...
UV LAMP 254 NM 8 WATT, 1 EA. Category: Nucleid Acid Detection Systems....
Biology Products: