Navigation Links
SlipChip counts molecules with chemistry and a cell phone
Date:11/18/2013

during the amplification process. The dRT-LAMP technique eliminates the need for continuous tracking of the intensity of fluorescence; instead, just one end-point readout measurement is used. The resulting patchwork of "positive" or "negative" wells on the device, in combination with statistical analysis, enables single molecules to be counted.

"In each well, you are performing a qualitative experiment; the result is like a pregnancy test: either yes or no, positive or negative, for the presence of an HIV RNA molecule," says David Selck, a graduate student in Ismagilov's lab and a first author on the study. "But by doing a couple of thousand qualitative experiments, you end up getting a numerical, quantitative result: the concentration of HIV RNA molecules in the sample. By calculating the concentration from the number of wells that contain fluorescenceand therefore HIVyou're leveraging the robustness of many qualitative 'yes or no' experiments to fulfill the need for a quantitative, numerical result," he says.

When the researchers compared quantification results from dRT-LAMP to those obtained by the real-time, kinetic version of this chemistry, RT-LAMP, they found that the digital format provided accurate results despite changes in temperature and time, while the kinetic format could not. This finding adds to a body of research that the laboratory has been developing on the robustness of converting analog signals (i.e., a readout reflecting a changing concentration over time) into a series of positive or negative digital signals. Another recent paper, published in the Journal of the American Chemical Society, explored a variation on this analog-to-digital conversion.

Ismagilov's group also tested a way to take an image of the fluorescence pattern in the wells of the SlipChip and, from that image, determine the viral loadwithout the use of expensive microscopes or trained staff. They turned to a nearly ubiquitous 21st-century tech
'/>"/>

Contact: Deborah Williams-Hedges
mr@caltech.edu
626-395-3227
California Institute of Technology
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. EyeLock Appoints Michael Fiorito Vice President of Enterprise Accounts
2. Quantum communication: Each photon counts
3. Global Biotech and Pharmaceutical Conference Early Bird Registration Discounts End This Week
4. Early Bird Discounts Available on International Healthcare and Biotechnology Conferences, Save Up To $600 with Global Information
5. Attendees Save Up To $800 on Boston-area Pharmaceutical and Healthcare Conferences, Early Bird Discounts Expiring April 27
6. Lawrence Livermore researchers unveil carbon nanotube jungles to better detect molecules
7. Biomolecules for the production line
8. CCNY chemists devise new way to prepare molecules for drug testing
9. Facebook for molecules
10. Detecting mirror molecules
11. Natural keystone molecules punch over their weight in ecosystems
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/27/2014)... 27, 2014 Reportlinker.com announces that a ... catalogue: Law Enforcement Biometrics Market in ... About Biometrics Biometrics is a ... basis of their physical or behavioral patterns. It ... traits such as fingerprints, face recognition, DNA, palm ...
(Date:8/27/2014)... Many African-Americans may not be getting effective doses of ... Hopkins suggests. The initial dosing studies, completed before the ... generally lack a protein that is key to removing ... people with maximum levels of the protein including ... maraviroc in their bodies compared to those who lack ...
(Date:8/27/2014)... the brain of mice, scientists have altered the ... by Howard Hughes Medical Institute investigator Susumu Tonegawa ... that the connections between the part of the ... and the part of the brain that stores ... , Altering those connections can transform a ...
Breaking Biology News(10 mins):Law Enforcement Biometrics Market in North America 2014-2018 2Law Enforcement Biometrics Market in North America 2014-2018 3Law Enforcement Biometrics Market in North America 2014-2018 4Law Enforcement Biometrics Market in North America 2014-2018 5Dosage of HIV drug may be ineffective for half of African-Americans 2Researchers change the emotional association of memories 2Researchers change the emotional association of memories 3Researchers change the emotional association of memories 4
... Faculty of Veterinary Medicine at the University of Pennsylvania, and ... and by Dr Lluís Tort of the Universitat Autònoma de ... cells in fish as well as in amphibians are capable ... experiments. The work has been published in Nature Immunology, the ...
... National Human Genome Research Institute (NHGRI), part of the ... round of grant awards totaling more than $13 million ... reduce the cost of DNA sequencing and expand the ... , "There has been significant progress over the ...
... led by Professor Nigel Dimmock at the University of ... against flu. This has been shown to protect ... protection against the full range of influenza A infections, ... infecting humans. The 'protecting virus' provides instant protection, and ...
Cached Biology News:Discovery in the evolution of the immune system absorbing cells 2NHGRI aims to make DNA sequencing faster, more cost effective 2NHGRI aims to make DNA sequencing faster, more cost effective 3NHGRI aims to make DNA sequencing faster, more cost effective 4NHGRI aims to make DNA sequencing faster, more cost effective 5NHGRI aims to make DNA sequencing faster, more cost effective 6'Protecting virus' offers instant flu protection and converts flu infections into their own vaccines 2'Protecting virus' offers instant flu protection and converts flu infections into their own vaccines 3
(Date:8/28/2014)... Vt. (PRWEB) August 28, 2014 ... testing around the world is available from Strategic ... diagnostics companies. “ Industrial Microbiology Market Review, Fourth ... Industrial Market ” (IMMR—4) tracks and compares microbiology ... in North America, Europe and Asia, and forecasts ...
(Date:8/28/2014)... Boston, MA (PRWEB) August 28, 2014 ... of wholesale construction materials, selected SoundConnect ... of their cloud based audio and web conferencing ... configured to support PrimeSource’s employees’ needs. SoundConnect ... technologies. , PrimeSource Building Products will be ...
(Date:8/28/2014)... New York, NY (PRWEB) August 28, 2014 ... has renewed its long term partnership with PTI ... to provide clients with state of the art leak ... state of the art instruments currently available. As part ... a new High Voltage Leak Detection Instrument ...
(Date:8/27/2014)... Austin, TX (PRWEB) August 27, 2014 ... $6 million in Series B funding and secured Otter ... and investment capital will accelerate commercialization of GGI’s Agriplier™ ... multiple crop types. , “Since our first meeting, ... commitment to the biological product space,” said Alan Sobba, ...
Breaking Biology Technology:2 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3PrimeSource Chooses SoundConnect as Collaboration Provider 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 3Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 2Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 3
... N.Y., Feb. 16, 2012   Health Care Compliance ... compliance and competency training courseware, today announced the ... CMS (Centers for Medicare and Medicaid Services) ICD-10 ... ) The switchover to the new ...
... Pa., Feb. 16, 2012  BioDelivery Sciences International, Inc. (Nasdaq: ... Trademark Office (USPTO) has issued a Notice of Allowance ... granted, will extend the exclusivity of the BioErodible MucoAdhesive ... Buprenorphine/Naloxone from 2020 to 2027.  A ...
... 16, 2012  Adeona Pharmaceuticals, Inc. (NYSE Amex: ... innovative disease-modifying medicines for serious illnesses, announced today that ... change its corporate name to Synthetic Biologics, Inc. It ... under its new name and stock ticker symbol, "SYN", ...
Cached Biology Technology:HCCS Releases ICD-10 Countdown Clock With Integrated CMS Twitter Tweets 2BioDelivery Sciences Receives Patent Allowance Triggering $15 Million Milestone Payment from Endo Pharmaceuticals 2BioDelivery Sciences Receives Patent Allowance Triggering $15 Million Milestone Payment from Endo Pharmaceuticals 3BioDelivery Sciences Receives Patent Allowance Triggering $15 Million Milestone Payment from Endo Pharmaceuticals 4BioDelivery Sciences Receives Patent Allowance Triggering $15 Million Milestone Payment from Endo Pharmaceuticals 5
...
A component of the Acridinium protein labeling kit....
... The MiniOpticon 2-color real-time PCR detection system ... 48-well microplate. An array of 48 LEDs independently ... a pair of filtered photodiodes detects emitted light ... built on a MJ Mini cycler, whose Peltier ...
... to hold up to 6 strain chambers with ... to use design ensures that cells grow over ... or compression. This unit allows you to control ... produce 64 unique strain settings. The instrument fits ...
Biology Products: