Navigation Links
Single-stranded DNA-binding protein is dynamic, critical to DNA repair
Date:10/21/2009

CHAMPAIGN, Ill. Researchers report that a single-stranded DNA-binding protein (SSB), once thought to be a static player among the many molecules that interact with DNA, actually moves back and forth along single-stranded DNA, gradually allowing other proteins to repair, recombine or replicate the strands.

Their study, of SSB in the bacterium Escherichia coli, appears today in the journal Nature.

Whenever the double helix of DNA unravels, exposing each strand to the harsh environment of the cell, SSB is usually first on the scene, said University of Illinois physics professor and Howard Hughes Medical Institute investigator Taekjip Ha, who led the study.

Although DNA unwinding is necessary for replication or recombination, it is normally a transient process, he said. Exposed single-stranded DNA (ssDNA) can be damaged or degraded by enzymes in the cell. Damaged DNA may also come unwound, and ssDNA can bond to itself, forming hairpin loops and other problematic structures.

"If you have lots of single-stranded DNA in the cell, basically it's a sign of trouble," Ha said. "SSB needs to come and bind to it to protect it from degradation and to control what kind of proteins have access to the single-stranded DNA."

Although other proteins are known to travel along double-stranded DNA, this is the first study to find a protein that migrates back and forth randomly on single-stranded DNA, Ha said.

Other researchers had assumed that SSB simply bound to DNA where it was needed and then fell off when its job was done. But a collaborator on the new study who has studied SSB for two decades, Timothy Lohman, of Washington University School of Medicine, suspected that the protein's interaction with DNA was more dynamic. That hunch turned out to be true, Ha said.

The SSB protein is made up of four identical subunits. Single-stranded DNA loops around and through them in a pattern "that looks like the s
'/>"/>

Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2

Related biology news :

1. University of the Basque Country study on proteins related to Alzheimers
2. InVivo and CEVEC pharmaceuticals sign license agreement regarding the use of human CAP-T Technology for production of recombinant proteins
3. How the 100th protein structure solved at Diamond impacts our understanding of how insects smell
4. All tied up: Tethered protein provides long-sought answer
5. How proteins talk to each other
6. Nanoresearchers challenge dogma in protein transportation in cells
7. Researchers prolong the half-life of biopharmaceutical proteins
8. Scientists pinpoint protein link to fat storage
9. UT scientists discover link between protein and lung disease
10. High-res view of zinc transport protein
11. When proteins change partners
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Single-stranded DNA-binding protein is dynamic, critical to DNA repair
(Date:7/24/2014)... NY - July 24, 2014 -- Keryx Biopharmaceuticals, Inc. ... the long-term, randomized, active control Phase 3 study of ... phosphate binder, for the treatment of hyperphosphatemia in patients ... study (PhosphatE binding and iRon delivery with FErric CiTrate ... Journal of the American Society of Nephrology ( ...
(Date:7/24/2014)... of native animal species for food or commercial food ... factor in the decline of many species of primates ... that more than half of the species being consumed ... "By surveying not only the meat made available for ... the forest by hunters and brought to villages for ...
(Date:7/24/2014)... study has identified two factors that characterize sustainable ... production of highly qualified physics teachers. Specifically, one ... physics teacher education in combination with institutional motivation ... viable. Science, Technology, Engineering and Math (STEM) teacher ... study points the way for institutions seeking to ...
Breaking Biology News(10 mins):Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 4Creating sustainable STEM teacher preparation programs 2
... drug for cocaine addiction and overdose, Journal of the ... what they term the most powerful substance ever discovered for ... to the world,s first effective medicine for fighting overdoses and ... the Sept. 24 issue of the Journal of the ...
... A researcher at Oregon State University has used ... to identify counties in Oregon with high numbers of ... in fighting the illegal drug. The study, presented ... from four sources then identified five counties with the ...
... a muscle fiber mesh at the neuromuscular junction. New work ... Cell Biology reveals that an extracellular matrix protein called ... fit together type="rel"doi="10.1083/jcb.2008. , A neuromuscular junction, or synapse, in ... nerve terminal meeting a flat, oval structure on the muscle ...
Cached Biology News:American Chemical Society's Weekly PressPac -- Sept. 10, 2008 2American Chemical Society's Weekly PressPac -- Sept. 10, 2008 3American Chemical Society's Weekly PressPac -- Sept. 10, 2008 4American Chemical Society's Weekly PressPac -- Sept. 10, 2008 5American Chemical Society's Weekly PressPac -- Sept. 10, 2008 6American Chemical Society's Weekly PressPac -- Sept. 10, 2008 7American Chemical Society's Weekly PressPac -- Sept. 10, 2008 8New method identifies meth hot spots 2New method identifies meth hot spots 3
(Date:7/24/2014)... 24, 2014  Now available is a stem cell ... Regenerative Therapies , located in Guadalajara, Mexico ... people a new health option: stem cell therapies ... countless patients suffer from joint and muscle injuries due ... tears, torn rotator cuff, tennis elbow, and knee injuries. ...
(Date:7/24/2014)... by group leader Yung-Eun Sung has announced that ... and nitrogen-doped graphenes which can be applied as ... cells. Yung-Eun Sung is both a group leader ... for Basic Science* (IBS) and a professor at ... great significance with regards to the development of ...
(Date:7/24/2014)... , July 24, 2014 Research and ... "Global Cell Culture Market 2014-2018" report to their ... Cell culture is the in vitro growth of ... research, cell studies, and biopharmaceutical production. Cell culture is ... Cell culture instruments and cell culture consumables are necessary ...
(Date:7/24/2014)... N.J. , July 24, 2014  CorMedix ... on developing and commercializing therapeutic products for the ... today announces receipt of notice on July 18, ... the NYSE-MKT has accepted the CorMedix plan to ... NYSE-MKT. As a result, the NYSE-MKT is continuing ...
Breaking Biology Technology:Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3Global Cell Culture Market 2014-2018: Key Vendors are Becton, Dickinson and Co, EMD, GE Healthcare, Lonza Group, Sigma Aldrich and Thermo Fisher Scientific 2CorMedix, Inc. Granted Extension of NYSE-MKT Listing 2CorMedix, Inc. Granted Extension of NYSE-MKT Listing 3
... BIRMINGHAM, Ala., Oct. 31 BioCryst,Pharmaceuticals, Inc. (Nasdaq: ... quarter ended September 30, 2008., Third Quarter ... ended September 30, 2008, the Company reported,collaborative and ... to $20.5 million for the three months ended ...
... and waves erode billions of tons of soil from ... plagued with excessive amounts of suspended sediment. According to ... the largest nonpoint source pollution in the environment. , ... obvious wind, water and wave forces breaking apart particles ...
... 30 AVAX Technologies, Inc.,(OTC Bulletin Board: AVXT) ... it closed a bridge loan financing pursuant to ... by the Amendment to Convertible,Note and Warrant Purchase ... Company sold convertible promissory notes,(the "Notes") in the ...
Cached Biology Technology:BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 2BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 3BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 4BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 5BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 6BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 7BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 8BioCryst Reports Third Quarter 2008 Financial Results and Provides Corporate Update 9Virginia Tech engineers identify conditions that initiate erosion 2Virginia Tech engineers identify conditions that initiate erosion 3AVAX Technologies, Inc. Announces Closing of Bridge Loan Financing 2
...
Request Info...
Streptomycin sulfate an aminoglycoside used for bacterial selection binds to the small ribosomal subunit and interferes with protein biosynthesis.21H39N7O12)2x3H2SO4Formula weight:...
Adducin beta (C-16)...
Biology Products: