Navigation Links
Single-molecule manipulation for the masses
Date:6/1/2010

Cambridge, Mass., June 2, 2010 Scientists have developed a new massively-parallel approach for manipulating single DNA and protein molecules and studying their interactions under force. The finding appears in the June 2 issue of Biophysical Journal.

The team of researchers from the Rowland Institute at Harvard University claim that their technique, which they call "single molecule centrifugation", offers dramatic improvements in throughput and cost compared with more established techniques.

"By combining a microscope and a centrifuge, forces can be applied to many molecules at once while simultaneously observing their nano-to-microscale motions," explains author Wesley P. Wong, a Principal Investigator at Rowland.

Recent technologies such as optical and magnetic tweezers and the Atomic Force Microscope (AFM) have enabled the mechanical manipulation of single molecules, leading to new insights in biological systems ranging from DNA replication to blood clotting.

However, the tools used to perform these experiments are often expensive and can be tedious and complicated to use, limiting their use among scientists.

The Harvard researchers aimed to solve these problems by developing an instrument they call the Centrifuge Force Microscope (CFM), which uses centrifugal force to manipulate molecules.

Developing the instrument involved miniaturizing a light microscope and safely rotating it at high speeds while maintaining precision and control.

Experiments involve tethering thousands of micron-sized "carrier" particles to a surface and observing their motion as the sample rotates to generate the centrifugal force.

"We're really excited about this new method," says co-author Ken Halvorsen, a postdoctoral fellow. "After doing tedious single-molecule experiments for years, we thought there had to be a better way. Now, instead of doing one experiment thousands of times we can do thousands of experiments at once."

The scientists expect that the relative low cost and simplicity of the method will attract researchers who may be intimidated by the cost and technical skills required for other methods, ultimately enabling new discoveries in both health and basic science research.


'/>"/>

Contact: Michael Patrick Rutter
mrutter@seas.harvard.edu
617-496-3815
Harvard University
Source:Eurekalert  

Related biology news :

1. Rice scientists make breakthrough in single-molecule sensing
2. Model for the assembly of advanced, single-molecule-based electronic components developed at Pitt
3. Flower power can still calm the masses
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Single-molecule manipulation for the masses
(Date:6/3/2016)... LONDON , June 3, 2016 /PRNewswire/ ... Transport Management) von Nepal ... ,Angebot und Lieferung hochsicherer geprägter Kennzeichen, einschließlich ... weltweit führend in der Produktion und Implementierung ... an der Ausschreibung im Januar teilgenommen, aber ...
(Date:6/2/2016)... June 2, 2016 Perimeter Surveillance ... Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... will generate revenues of $17.98 billion in 2016. ... Inc, a leader in software and hardware technologies for ...
(Date:5/20/2016)... 20, 2016  VoiceIt is excited to announce ... By working together, VoiceIt and VoicePass ... and VoicePass take slightly different approaches to voice ... security and usability. ... new partnership. "This marketing and technology ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... SILVER SPRING, Md. , June 23, 2016 ... evidence collected from the crime scene to track the criminal ... sick, and the U.S. Food and Drug Administration (FDA) uses ... Sound far-fetched? It,s not. ... whole genome sequencing to support investigations of foodborne illnesses. Put ...
(Date:6/23/2016)... 2016  The Biodesign Challenge (BDC), a university competition ... harness living systems and biotechnology, announced its winning teams ... New York City . The ... projects at MoMA,s Celeste Bartos Theater during the daylong ... senior curator of architecture and design, and Suzanne ...
(Date:6/23/2016)... ... , ... In a new case report published today in STEM CELLS Translational ... lymphedema after being treated for breast cancer benefitted from an injection of stem cells ... this debilitating, frequent side effect of cancer treatment. , Lymphedema refers to ...
(Date:6/23/2016)... YORK , June 23, 2016 ... trading session at 4,833.32, down 0.22%; the Dow Jones Industrial ... S&P 500 closed at 2,085.45, down 0.17%. Stock-Callers.com has initiated ... INFI ), Nektar Therapeutics (NASDAQ: NKTR ), ... Therapeutics Inc. (NASDAQ: BIND ). Learn more about ...
Breaking Biology Technology: