Navigation Links
Single-molecule manipulation for the masses
Date:6/1/2010

Cambridge, Mass., June 2, 2010 Scientists have developed a new massively-parallel approach for manipulating single DNA and protein molecules and studying their interactions under force. The finding appears in the June 2 issue of Biophysical Journal.

The team of researchers from the Rowland Institute at Harvard University claim that their technique, which they call "single molecule centrifugation", offers dramatic improvements in throughput and cost compared with more established techniques.

"By combining a microscope and a centrifuge, forces can be applied to many molecules at once while simultaneously observing their nano-to-microscale motions," explains author Wesley P. Wong, a Principal Investigator at Rowland.

Recent technologies such as optical and magnetic tweezers and the Atomic Force Microscope (AFM) have enabled the mechanical manipulation of single molecules, leading to new insights in biological systems ranging from DNA replication to blood clotting.

However, the tools used to perform these experiments are often expensive and can be tedious and complicated to use, limiting their use among scientists.

The Harvard researchers aimed to solve these problems by developing an instrument they call the Centrifuge Force Microscope (CFM), which uses centrifugal force to manipulate molecules.

Developing the instrument involved miniaturizing a light microscope and safely rotating it at high speeds while maintaining precision and control.

Experiments involve tethering thousands of micron-sized "carrier" particles to a surface and observing their motion as the sample rotates to generate the centrifugal force.

"We're really excited about this new method," says co-author Ken Halvorsen, a postdoctoral fellow. "After doing tedious single-molecule experiments for years, we thought there had to be a better way. Now, instead of doing one experiment thousands of times we can do thousands of experiments at once."

The scientists expect that the relative low cost and simplicity of the method will attract researchers who may be intimidated by the cost and technical skills required for other methods, ultimately enabling new discoveries in both health and basic science research.


'/>"/>

Contact: Michael Patrick Rutter
mrutter@seas.harvard.edu
617-496-3815
Harvard University
Source:Eurekalert  

Related biology news :

1. Rice scientists make breakthrough in single-molecule sensing
2. Model for the assembly of advanced, single-molecule-based electronic components developed at Pitt
3. Flower power can still calm the masses
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Single-molecule manipulation for the masses
(Date:4/4/2017)... , April 4, 2017   EyeLock LLC , ... that the United States Patent and Trademark Office (USPTO) ... covers the linking of an iris image with a ... and represents the company,s 45 th issued patent. ... is very timely given the multi-modal biometric capabilities that ...
(Date:3/30/2017)... March 30, 2017 Trends, opportunities and forecast ... behavioral), by technology (fingerprint, AFIS, iris recognition, facial recognition, ... others), by end use industry (government and law enforcement, ... and banking, and others), and by region ( ... Asia Pacific , and the Rest ...
(Date:3/24/2017)... Controller General of Immigration from Maldives Mr. Mohamed Anwar and ... international IAIR Award for the most innovative high security ePassport and eGates  ... ... Maldives Immigration Controller General, Mr. Mohamed Anwar ... right) have received the IAIR award for the "Most innovative high security ...
Breaking Biology News(10 mins):
(Date:6/26/2017)... ... , ... Third Wave Bioactives, LLC announces the addition of Brett Thompson. Brett ... development and ensuring quality customer experience. , Brett brings to Third Wave ... marketing and sales roles. “Brett’s background working with customers and eye for market opportunities ...
(Date:6/23/2017)... Frederick, MD (PRWEB) , ... June 23, 2017 ... ... laboratory software solutions provider, announced the latest version of LimitLIS®, its rapidly growing ... designed to speed up user adoption, ensure installation integrity, and provide more customization ...
(Date:6/22/2017)... ... June 21, 2017 , ... Beaker, the industry’s pioneer ... the life sciences industry, today announces a strategic partnership with Alcami Corporation, a ... advantage of Beaker’s expertise in executive recruitment solutions, providing Alcami with access to ...
(Date:6/22/2017)... (PRWEB) , ... June 22, 2017 , ... ... health leaders in designating infertility as a disease, bringing new hope for prospective ... at their 2017 annual meeting to back the World Health Organization’s designation in ...
Breaking Biology Technology: