Navigation Links
Single-cell transfection tool enables added control for biological studies
Date:5/21/2013

nology developed in Espinosa's lab. The NFP-E chip consists of an array of microfabricated cantilever probes with integrated microfluidic channels. The probe has previously been used for high-speed nanopatterning of proteins and nanoparticles for drug delivery studies.

The new single-cell transfection application couples the probe with an electrode and fluid control system that can be easily connected to a micromanipulator or atomic force microscope for position control. This integrated system allows the entire transfection process and post-transfection cell response to be monitored by an optical microscope.

The NFP-E system is being developed for commercialization by iNfinitesimal LLC, a Northwestern spin-off company founded by Espinosa, and is expected to be available in late 2013.

The technique is proving to be extremely robust and multi-functional. Researchers have used the NFP-E chip to transfect HeLa cells with polysaccharides, proteins, DNA hairpins, and plasmid DNA with single-cell selectivity, high transfection efficiency (up to 95%), qualitative dosage control, and very high viability (up to 92%).


'/>"/>

Contact: Megan Fellman
fellman@northwestern.edu
847-491-3115
Northwestern University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Nanobubbles plus chemotherapy equals single-cell cancer targeting
2. Single-cell parasites co-opt ready-made genes from host: UBC research
3. DNA analysis aids in classifying single-celled algae
4. They hunt, they kill, they cheat: Single-celled algae shed light on social lives of microbes
5. Keck award enables Carnegie Mellon and Stanford to dramatically expand crowdsourced RNA design
6. New genomic sequencing method enables smarter anaysis of individual cells
7. New technique enables high-sensitivity view of cellular functions
8. Aware Enables Automated Delivery of Radiation Dose Information Through Integration With Leading Radiology Reporting System
9. Mutation altering stability of surface molecule in acid enables H5N1 infection of mammals
10. Fish oil added to yogurt may help consumers meet daily nutritional requirements
11. Virginia Tech announces 2012 football helmet ratings; 2 more added to the 5-star mark
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/25/2015)... BELLEVUE, Wash. , Aug. 25, 2015 ... systems, announced today it will unveil its "Guardian S" ... at the National Tactical Officers Association Conference on August ... Guardian S is the first-ever commercially available energetically autonomous ... research and in-field trials and is protected by more ...
(Date:8/20/2015)... , Aug. 20, 2015   Sensory, ... on improving the user experience and security of ... technologies, today announced that its TrulySecure™ ... authentication software to be FIDO Certified™. ... for compliance with the FIDO UAF (Universal Authentication ...
(Date:8/18/2015)... DUBLIN , Aug. 17, 2015 ... the addition of the "Global Biometric Authentication ... Application - Estimation & Forecast, 2015-2020" report ... biometric authentication and identification systems market is expected ... from 2015 to 2020 and generate over $25 ...
Breaking Biology News(10 mins):Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 2TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 4TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 5Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2
... voices is second nature to humans. Certain primates also ... social groups are also able to do so, however, ... for meerkats. They coordinate their activities with calls, such ... for instance, and thus stick together as a group. ...
... publishing workflow of eight journals published by Pensoft has ... data archiving for data files associated with articles in these journals. ... the online editorial management platform, Pensoft Journal System (PJS). The ... MycoKeys , BioRisk , NeoBiota , Nature Conservation ...
... Studies have estimated that converting manure from the 95 million ... equal to 8 billion gallons of gasoline, or 1% of ... and more farmers and communities are interested in generating renewable ... information on the economic feasibility and sustainability of such programs. ...
Cached Biology News:Meerkats recognize each other from their calls 2Data publishing in Pensoft journals integrated with the Dryad Data Repository 2Does converting cow manure to electricity pay off? 2
(Date:8/31/2015)... - Portage Biotech Inc. ("Portage" or "the Company") (OTC ... announce that its wholly owned subsidiary, Portage Pharmaceuticals Ltd. ... an Investigational New Drug (IND) application for the topical ... completed a study in a rat model of dry ... efficacy and a more rapid onset of action than ...
(Date:8/31/2015)... , Aug. 31, 2015 LabStyle Innovations ... Management Solution, today announced it recently closed a ... will be used to support the global rollout ... a mobile, cloud-based, diabetes management solution that includes ... pocket-sized, blood glucose monitoring device, and for working ...
(Date:8/31/2015)... ... August 31, 2015 , ... Proove Biosciences, Inc. is a ... Annual High Tech Awards. , Now in its 22nd year, this premier awards ... innovation here, across the nation and around the world. The OC Tech Alliance will ...
(Date:8/28/2015)... PA (PRWEB) , ... August 28, 2015 , ... ... Chairman, Chuck Gardner, has organized and will present a session on chemical spectroscopic ... 2. This conference and exposition is Asia’s largest analytical and scientific instruments show ...
Breaking Biology Technology:Portage's PPL will advance its lead candidate PPL-003 to an IND for dry eye disease and uveitis 2Portage's PPL will advance its lead candidate PPL-003 to an IND for dry eye disease and uveitis 3LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 2LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 3LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 4Proove Biosciences, Inc. Named a Finalist for 22nd OC Tech Alliance High-Tech Awards for Company of the Year 2Proove Biosciences, Inc. Named a Finalist for 22nd OC Tech Alliance High-Tech Awards for Company of the Year 3Proove Biosciences, Inc. Named a Finalist for 22nd OC Tech Alliance High-Tech Awards for Company of the Year 4U.S. Symposium on Imaging Organized by Pittcon’s Program Chairman Chuck Gardner 2U.S. Symposium on Imaging Organized by Pittcon’s Program Chairman Chuck Gardner 3
... Venture: QPT, EDMONTON, June 2 /PRNewswire-FirstCall/ - ... today announced that its 12-patient,Phase I clinical study ... for,the Photodynamic Therapy of Actinic Keratosis" has met ... delighted to have received, for the first time, ...
... (TSX: GEN), a,company focused on developing blood-based biomarker ... health management, today announced,management changes to support the ... May 31, 2008, Dr. K. Wayne Marshall has ... duties as Chief Clinical Scientist for GeneNews. Dr.,Marshall ...
... Tests identify genetic mutations that can affect patient response to ... ... Roche and DxS Ltd. have,signed an exclusive distribution agreement for the ... tests are,intended, when considered with other clinically relevant factors, to aid,doctors ...
Cached Biology Technology:Quest PharmaTech Announces Positive Results from Phase I Clinical Trial of its Photodynamic Therapy for Actinic Keratosis 2Quest PharmaTech Announces Positive Results from Phase I Clinical Trial of its Photodynamic Therapy for Actinic Keratosis 3GeneNews announces management changes to support commercialization of ColonSentry(TM) 2Roche Signs Exclusive Distribution Deal with DxS for K-RAS and EGFR Cancer Mutation Tests 2Roche Signs Exclusive Distribution Deal with DxS for K-RAS and EGFR Cancer Mutation Tests 3
... Nepheloskan Ascent is the first ... in microplates. Nepheloskan measures light ... technique for particle quantification. This ... testing in drug discovery, immunoprecipitation, ...
Anti-VDAC (Voltage-Dependent Anion Channel) AVOID FREEZE/THAW CYCLES. Recognizes the porin 31HL protein. Corresponds to mAb 1 in the literature....
6 x Blend of rTth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 proofreading activity...
... are used to assess the effect of drugs ... mice and rats using one unit in a ... animals are placed on textured drums to avoid ... sensing platforms below, its test results are recorded. ...
Biology Products: