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Single-cell transfection tool enables added control for biological studies
Date:5/21/2013

nology developed in Espinosa's lab. The NFP-E chip consists of an array of microfabricated cantilever probes with integrated microfluidic channels. The probe has previously been used for high-speed nanopatterning of proteins and nanoparticles for drug delivery studies.

The new single-cell transfection application couples the probe with an electrode and fluid control system that can be easily connected to a micromanipulator or atomic force microscope for position control. This integrated system allows the entire transfection process and post-transfection cell response to be monitored by an optical microscope.

The NFP-E system is being developed for commercialization by iNfinitesimal LLC, a Northwestern spin-off company founded by Espinosa, and is expected to be available in late 2013.

The technique is proving to be extremely robust and multi-functional. Researchers have used the NFP-E chip to transfect HeLa cells with polysaccharides, proteins, DNA hairpins, and plasmid DNA with single-cell selectivity, high transfection efficiency (up to 95%), qualitative dosage control, and very high viability (up to 92%).


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Contact: Megan Fellman
fellman@northwestern.edu
847-491-3115
Northwestern University
Source:Eurekalert

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