Navigation Links
Single-cell transfection tool enables added control for biological studies
Date:5/21/2013

Northwestern University researchers have developed a new method for delivering molecules into single, targeted cells through temporary holes in the cell surface. The technique could find applications in drug delivery, cell therapy, and related biological fields.

Bulk electroporation a technique used to deliver molecules into cells through reversible nanopores in the cell membrane that are caused by exposing them to electric pulses is an increasingly popular method of cell transfection. (Cell transfection is the introduction of molecules, such as nucleic acids or proteins, into a cell to change its properties.)

However, because bulk electroporation applies electric pulses to a bulk cell solution, it results in heterogeneous cell populations and often low cell viability. To solve these problems, Northwestern University researchers have developed a novel tool for single-cell transfection.

The new method, called nanofountain probe electroporation (NFP-E), allows researchers to deliver molecules into targeted cells through temporary nanopores in the cell membrane created by a localized electric field applied to a small portion of the cell. The method enables researchers to control dosage by varying the duration of the electric pulses, which provides unprecedented control of cell transfection.

"This is really exciting," said Horacio Espinosa, James and Nancy Farley Professor of Manufacturing and Entrepreneurship at Northwestern's McCormick School of Engineering and one of the paper's authors. "The ability to precisely deliver molecules into single cells is needed for biotechnology researchers to advance the state-of-the-art in therapeutics, diagnostics, and drug delivery toward the promise of personalized medicine."

A paper describing the research, "Nanofountain Probe Electroporation (NFP-E) of Single Cells," was published May 7 in the journal Nano Letters.

NFP-E is based on nanofountain probe (NFP) tech
'/>"/>

Contact: Megan Fellman
fellman@northwestern.edu
847-491-3115
Northwestern University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Nanobubbles plus chemotherapy equals single-cell cancer targeting
2. Single-cell parasites co-opt ready-made genes from host: UBC research
3. DNA analysis aids in classifying single-celled algae
4. They hunt, they kill, they cheat: Single-celled algae shed light on social lives of microbes
5. Keck award enables Carnegie Mellon and Stanford to dramatically expand crowdsourced RNA design
6. New genomic sequencing method enables smarter anaysis of individual cells
7. New technique enables high-sensitivity view of cellular functions
8. Aware Enables Automated Delivery of Radiation Dose Information Through Integration With Leading Radiology Reporting System
9. Mutation altering stability of surface molecule in acid enables H5N1 infection of mammals
10. Fish oil added to yogurt may help consumers meet daily nutritional requirements
11. Virginia Tech announces 2012 football helmet ratings; 2 more added to the 5-star mark
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/22/2014)... , Dec. 22, 2014 Research and Markets ... addition of the "The Global Watermarking and ... http://photos.prnewswire.com/prnh/20130307/600769 ... digital media watermarking and fingerprinting markets. Watermarking aims ...
(Date:12/19/2014)... and Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ) has announced ... - Apple/AuthenTec TMDR92 & Sapphire - Technology Report" ... http://photos.prnewswire.com/prnh/20130307/600769 Following the acquisition of AuthenTec ... with the iPhone 5S. It is currently the only ...
(Date:12/19/2014)... Dec. 18, 2014  23andMe, Inc., the leading personal genetics company, ... differences in genetic ancestry of individuals from across ... arrived more than four hundred years ago, the ... for peoples from different continents. This study illuminates how American ...
Breaking Biology News(10 mins):The Global Watermarking and Fingerprinting Markets 2iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 223andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4
... Office of Science has established a program to ... Katrina. , "Our colleagues in science have historically ... L. Orbach, director of DOE's Office of Science, ... universities and colleges, including those in Alabama, Louisiana ...
... Analysis of a key protein in different subtypes of ... drug amantadine in H5N1 occurs worldwide, but is especially ... to the antiviral drug amantadine is spreading more rapidly ... Asia than in North America, according to the study ...
... A new website with a Global Information System will ... Hurricane Katrina. The National Institute of Environmental Health Sciences ... the website to provide the most up-to-date data to ... waters, infrastructure and industry maps, as well as demographic ...
Cached Biology News:DOE's Office of Science sets up program to aid scientists displaced by Hurricane Katrina 2Drug resistant avian influenza viruses more common in Southeast Asia than North America 2Drug resistant avian influenza viruses more common in Southeast Asia than North America 3Drug resistant avian influenza viruses more common in Southeast Asia than North America 4FDA approves child-friendly AIDS medicine 2
(Date:1/22/2015)... January 22, 2015 Diagenode, Inc., ... as the Bioruptor® and complete solutions for epigenetics ... chromatin immunoprecipitation, alleviating the need for manual processing. ... needed for ChIP of histones or transcription factors ...
(Date:1/22/2015)... 2015   Cypher Genomics, Inc., the leading ... SQNM ), the leading molecular diagnostics company, ... noninvasive prenatal tests (NIPT). Through this agreement, Sequenom ... to advance analysis of clinically-relevant fetal sub-chromosomal variants ...
(Date:1/22/2015)...   GenoSpace , a precision medicine software company that has ... use of genomic, imaging and other biomedical data in research ... Munson , CEO of Aspera, an IBM Company, to its ... http://photos.prnewswire.com/prnh/20150122/170713 "We are pleased to welcome ...
(Date:12/25/2014)... (PRWEB) December 25, 2014 The report ... by Product Type & Provider Type - Global Advancements, ... market with an in-depth analysis and forecasting of revenues. ... spread through 221 pages and in-depth TOC on “Service ...
Breaking Biology Technology:Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4GenoSpace Expands Board with Appointment of Michelle Munson 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4
... Inc. (CTi), a federal government professional consulting firm, announced ... web-based services in support of DoD,s Blast Injury Research ... International Corporation (NYSE: SAI ) and the ... U.S. Army Medical Research and Materiel Command (USAMRMC), CTi ...
... extensive experience in multi-GPU high-performance computing systems ... provide scientists, engineers and researchers with a ... Supercomputer (PSC), which will be shown at ... SC08 Conference, the top international conference for ...
... ParkwayHealth is the first healthcare group in Southeast Asia to offer ... , ... Singapore (PRWEB) November 18, 2008 -- Singapore,s ParkwayHealth ... In-Space percutaneous interspinous distraction device, a revolutionary spinal procedure ., ...
Cached Biology Technology:Cascades Technologies, Inc. Chosen to Deliver IT Support on a DoD Blast Injury Research Program 2Cascades Technologies, Inc. Chosen to Deliver IT Support on a DoD Blast Injury Research Program 3BOXX Personal Supercomputer for Research Scientists Featured at SC08 2Singapore Medicine - Singapore Doctors Use Hi-Tech Implant to Add Space to Narrowed Spinal Canals 2Singapore Medicine - Singapore Doctors Use Hi-Tech Implant to Add Space to Narrowed Spinal Canals 3Singapore Medicine - Singapore Doctors Use Hi-Tech Implant to Add Space to Narrowed Spinal Canals 4
... The CellSensor CRE-bla HEK 293T cell ... control of the cAMP response element (CRE). ... construct was transduced into HEK 293T cells ... to isolate clones responsive to forskolin stimulation ...
1, 2 or 8 PreMixes are included as part of the FailSafe™ PCR System....
1, 2 or 8 PreMixes are included as part of the FailSafe™ PCR System....
Request Info...
Biology Products: