Navigation Links
Single-cell genome sequencing gets better
Date:11/10/2013

Researchers led by bioengineers at the University of California, San Diego have generated the most complete genome sequences from single E. coli cells and individual neurons from the human brain. The breakthrough comes from a new single-cell genome sequencing technique that confines genome amplification to fluid-filled wells with a volume of just 12 nanoliters.

The study is published in the journal Nature Biotechnology on November 10, 2013.

"Our preliminary data suggest that individual neurons from the same brain have different genetic compositions. This is a relatively new idea, and our approach will enable researchers to look at genomic differences between single cells with much finer detail," said Kun Zhang, a professor in the Department of Bioengineering at the UC San Diego Jacobs School of Engineering and the corresponding author on the paper.

The researchers report that the genome sequences of single cells generated using the new approach exhibited comparatively little "amplification bias," which has been the most significant technological obstacle facing single-cell genome sequencing in the past decade. This bias refers to the fact that the amplification step is uneven, with different regions of a genome being copied different numbers of times. This imbalance complicates many downstream genomic analyses, including assembly of genomes from scratch and identifying DNA content variations among cells from the same individual.

Single-cell Genome Sequencing

Sequencing the genomes of single cells is of great interest to researchers working in many different fields. For example, probing the genetic make-up of individual cells would help researchers identify and understand a wide range of organisms that cannot be easily grown in the lab from the bacteria that live within our digestive tracts and on our skin, to the microscopic organisms that live in ocean water. Single-cell genetic studies are als
'/>"/>

Contact: Daniel Kane
dbkane@ucsd.edu
858-534-3262
University of California - San Diego
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Nanobubbles plus chemotherapy equals single-cell cancer targeting
2. Single-cell parasites co-opt ready-made genes from host: UBC research
3. DNA analysis aids in classifying single-celled algae
4. They hunt, they kill, they cheat: Single-celled algae shed light on social lives of microbes
5. Single-cell transfection tool enables added control for biological studies
6. Leading evolutionary scientist to discuss how genome of bacteria has evolved
7. Darwin in the genome
8. Analysis of stickleback genome sequence catches evolution in action
9. Athletic frogs have faster-changing genomes
10. PNAS: Precise molecular surgery in the plant genome
11. BGI and Aspera collaborate on high-speed data exchange to advance genome research
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Single-cell genome sequencing gets better
(Date:4/17/2014)... trials to treat tuberculosis could be the basis for ... bacteria, fungal infections and parasites, yet evade resistance, according ... collaborators. , Led by U. of I. chemistry professor ... drug SQ109 attacks the tuberculosis bacterium, how the drug ... to malaria and how targeting multiple pathways reduces ...
(Date:4/17/2014)... modeling study suggests that fish consumption advisories for ... to long-lived contaminants like persistent organic pollutants (POPs). ... of researchers including University of Toronto Scarborough PhD ... at how different levels of environmental contamination, a ... chemicals in the body influenced exposure in her ...
(Date:4/17/2014)... University engineer has developed a patented technique that ... devices. The same technique could help police during ... and Kay L. Theede chair in engineering and ... his research team have created a template-based system ... trunks. The distance detection method called stand-off ...
Breaking Biology News(10 mins):Multitarget TB drug could treat other diseases, evade resistance 2Fish consumption advisories fail to cover all types of contaminants 2Patented research remotely detects nitrogen-rich explosives 2
... Sharing is a behavior on which day care workers and kindergarten ... for our ape cousins the bonobos, sharing just comes naturally. ... Current Biology, it looks like bonobos never seem to ... notorious for hogging food to themselves, by physical aggression if necessary. ...
... Imagine that you are emerging from the subway and heading ... in the wrong direction. For a moment, you feel disoriented, ... surrounding streets quickly helps you pinpoint your location, and you ... Research tells us that human adults, toddlers, rats, chicks ...
... the first to design a bioactive nanomaterial that promotes the ... of expensive growth factors. Minimally invasive, the therapy activates the ... therapy can do this. The results will be published ... of the National Academy of Sciences (PNAS). "Unlike ...
Cached Biology News:'Peter Pan' apes never seem to learn selfishness 2'Peter Pan' apes never seem to learn selfishness 3Ability to navigate may be linked to genes, researcher says 2Ability to navigate may be linked to genes, researcher says 3Growing cartilage -- no easy task 2
(Date:1/14/2014)... Toronto, Canada (PRWEB) January 14, 2014 ... and its motives and methods in product development and ... of the industry. This mistrust, fueled by concerns about ... further fed by reports of spectacular fines to the ...
(Date:1/14/2014)... Kinaxis ®, provider of RapidResponse ®, a cloud-based supply ... ( S&OP ) service, is proud to be a gold ... held at the Hilton San Diego Bayfront, January 30 – ... Kaszas , Director of Supply Chain at Amgen, as she ...
(Date:1/14/2014)... During the 1600’s through the 1800’s doctors contributed unknowingly to ... time period, doctors did not know that they needed to ... of vulnerable patients. In the same way, medical interns and ... transmitting herpes viruses to their patients. The CBCD explains. , ...
(Date:1/14/2014)... Springfield, MO (PRWEB) January 14, 2014 ... a comprehensive database of material test reports (MTRs) for ... and fabricates. Documenting test results is particularly important for ... other regulations. The MTR Library features search functionality that ...
Breaking Biology Technology:The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3Pressure Vessel Design and Fabrication Company Builds Database for Material Test Reports 2Pressure Vessel Design and Fabrication Company Builds Database for Material Test Reports 3
... JOLLA, Calif., Nov. 22, 2011  If algae is to be ... workers are needed to make that a reality. Applications are ... California San Diego to retrain workers as general science technicians ... and Imperial County region. Approximately 55 students ...
... Sequenom, Inc. (NASDAQ: SQNM ), a life ... Company,s participation at the 23rd Annual Piper Jaffray Healthcare Conference ... 2011. Ronald M. Lindsay, Ph.D., Director and ... will present on Tuesday, November 29, 2011 beginning at 8:30 ...
... Among the many hurdles to be cleared before human embryonic ... or not transplanted cells can functionally integrate into target organs ... Proceedings of the National Academy of Sciences, a ... lab from blank slate human embryonic stem cells and implanted ...
Cached Biology Technology:Free Job Training: 55 Grants Available to Retrain Workers for Biofuels Industry 2Free Job Training: 55 Grants Available to Retrain Workers for Biofuels Industry 3Sequenom Announces Participation at the 23rd Annual Piper Jaffray Healthcare Conference 2Implanted neurons, grown in the lab, take charge of brain circuitry 2Implanted neurons, grown in the lab, take charge of brain circuitry 3
...
... Signet offers the most comprehensive, cost-effective ... We have incorporated unprecedented quality into ... This includes proven detection chemistries and ... the TechMate protocol. It also includes ...
... Signets Xylene Resistant (X/R) Red System ... X/R Red Activator and X/R Red ... with alkaline phosphatase to produce red ... ,Kit Contains:, , Buffer, 100 ...
Human Cell Line Slides...
Biology Products: