Navigation Links
Single-cell analysis holds promise for stem cell and cancer research
Date:8/7/2014

UC San Francisco researchers have identified cells' unique features within the developing human brain, using the latest technologies for analyzing gene activity in individual cells, and have demonstrated that large-scale cell surveys can be done much more efficiently and cheaply than was previously thought possible.

"We have identified novel molecular features in diverse cell types using a new strategy of analyzing hundreds of cells individually," said Arnold Kriegstein, MD, PhD, director of the Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research at UCSF. "We expect to use this approach to help us better understand how the complexity of the human cortex arises from cells that are spun off through cell division from stem cells in the germinal region of the brain."

The research team used technology focused on a "microfluidic" device in which individual cells are captured and flow into nano-scale chambers, where they efficiently and accurately undergo the chemical reactions needed for DNA sequencing. The research showed that the number of reading steps needed to identify and spell out unique sequences and to successfully identify cell types is 100 times fewer than had previously been assumed. The technology, developed by Fluidigm Corporation, can be used to individually process 96 cells simultaneously.

"The routine capture of single cells and accurate sampling of their molecular features now is possible," said Alex Pollen, PhD, who along with fellow Kriegstein-lab postdoctoral fellow Tomasz Nowakowski, PhD, conducted the key experiments, in which they analyzed the activation of genes in 301 cells from across the developing human brain. Their results were published online August 3 in Nature Biotechnology.

Kriegstein said the identification of hundreds of novel biomarkers for diverse cell types will improve scientists' understanding of the emergence of specialized neuronal subtypes. Ultimately, th
'/>"/>

Contact: Jeffrey Norris
jeffrey.norris@ucsf.edu
415-502-6397
University of California - San Francisco
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Microchip-like technology allows single-cell analysis
2. Single-cell genome sequencing gets better
3. Single-cell transfection tool enables added control for biological studies
4. They hunt, they kill, they cheat: Single-celled algae shed light on social lives of microbes
5. DNA analysis aids in classifying single-celled algae
6. Single-cell parasites co-opt ready-made genes from host: UBC research
7. Nanobubbles plus chemotherapy equals single-cell cancer targeting
8. 15-year analysis of blue whale range off California finds conflict with shipping lanes
9. Global Next Generation Biometric Market by Technology, Function, Application & by Geography - Forecasts & Analysis to 2020
10. International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019
11. Shark teeth analysis provides detailed new look at Arctic climate change
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/16/2014)... 2014)The North American Menopause Society (NAMS) has published ... of midlife womenon everything from hot flashes to ... Menopause Society Recommendations for Clinical Care of Midlife ... journal Menopause . This is the first, ... of midlife women freely available to all clinicians ...
(Date:9/16/2014)... September 13, 2014. The fire is human-caused and ... acres in size and is mostly being fueled by ... Monday night due to higher humidity. Yesterday (8/15), ... to construct a fireline on the West and North sides ... the fire: , 7 20-person Crews, 4 Hot Shot Crews, ...
(Date:9/16/2014)... has its own bacterial identity. That,s the conclusion of ... who studied the genetic fingerprints of bacteria on 57 ... "This study demonstrates for the first time that host ... strategies possess very different microbial communities on their leaves," ... researcher in the UO,s Institute of Ecology and Evolution ...
Breaking Biology News(10 mins):Microbiome research shows each tree species has a unique bacterial identity 2
... of Leicester have identified for the first time a new ... in our blood following a meal. They have ... to maintain correct blood sugar levels. The breakthrough was ... by Professor Andrew Tobin, Professor of Cell Biology, who is ...
... of many countries in Europe and Asia. Great Tits ... long been known individuals vary considerably in their willingness to ... or "proactive" are quick explorers and are comparatively aggressive, ... differences are at least in part genetically determined and as ...
... be able to receive more effective, personalized treatments for ... cancer biology, experts will say at the Cancer Biology ... Medical Oncology (ESMO) in Nice this week. ... transformation, governments, pharmaceutical companies and doctors urgently need to ...
Cached Biology News:Bird-brained? Birds' personalities are correlated with their hormone levels 2New strategies for cancer drug development urgently needed 2New strategies for cancer drug development urgently needed 3
(Date:9/16/2014)...  The Council for Entrepreneurial Development (CED), the ... country, today released a mid-year update to its ... It showed that in the first half of ... in the technology, life science, advanced manufacturing and ... variety of sources. Significant investment in technology companies and ...
(Date:9/16/2014)... September 16, 2014 Convoy Therapeutics, ... and effective products to treat patients directly at ... a proprietary SPACEā„¢ Technology Platform announced today that ... will present at the White Hat Life Science ... Hyatt Regency Phoenix. In addition, interested individuals ...
(Date:9/16/2014)...  Ascendis Pharma A/S, a biotechnology company that ... unmet medical needs, today announced positive interim results ... evaluate once-weekly TransCon Growth Hormone in children with ... results will be presented at the 7th International ... held October 15-18, 2014, in Singapore.  ...
(Date:9/16/2014)... 16, 2014 According to Jeff Howell, ... of ITRA Global, new development in Downtown Toronto is ... council approving another 755 storeys of new development last ... cranes will be dominating the Toronto skyline for the ... an unquenchable thirst for new development. , As the ...
Breaking Biology Technology:CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 3CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 4Convoy Therapeutics to Present at WhiteHat Investor Conference September 18, 2014 2Convoy Therapeutics to Present at WhiteHat Investor Conference September 18, 2014 3Ascendis Pharma A/S Announces Positive Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 3
... ... and Roles , ... Canberra, Australia (Vocus) October 27, 2009 -- Global food security, which is increasingly being ... and executed public and private partnerships, William S. Niebur , vice president – ...
... Oct. 27 /PRNewswire-FirstCall/ - Oncolytics Biotech Inc. (TSX: ... that it has been granted its 33rd U.S. ... Resistant Neoplastic Cells With a Virus." The patent ... with currently approved chemotherapeutic agents to treat patients ...
... Oct. 27 Precision Antibody(TM) has been selected ... National Cancer Institute (NCI),s Clinical Proteomic Technologies for Cancer ... standardizing the quality of monoclonal antibody reagents available for ... result of a third solicitation for contract proposals from ...
Cached Biology Technology:Public-Private Partnerships Key to Achieving Global Food Security, Says DuPont Leader 2Public-Private Partnerships Key to Achieving Global Food Security, Says DuPont Leader 3Oncolytics(R) Biotech Inc. Announces Issuance of 33rd U.S. Patent 2Precision Antibody Wins Contract to Develop 'Gold Standard' Antibodies as Part of National Cancer Institute's Clinical Proteomics Technologies for Cancer (CPTC) Initiative 2Precision Antibody Wins Contract to Develop 'Gold Standard' Antibodies as Part of National Cancer Institute's Clinical Proteomics Technologies for Cancer (CPTC) Initiative 3
Request Info...
... Tissue Array (MSTA) slides were designed to study ... from multiple species. Tissue slices from three ... which can then be treated as a single ... in situ hybridization. This format allows ...
... Pulser Xcell microbial system, 100 to ... for electroporation of bacterial and fungal ... unit, PC module for exponential decay ... cuvettes (5 each of 0.1 and ...
... (TSB), USP is a highly nutritious versatile ... use. Due to the inclusion of both ... support growth of many fastidious organisms without ... conforms to United States Pharmacopeia standards for ...
Biology Products: