Navigation Links
Single adult stem cell can self renew, repair tissue damage in live mammal
Date:12/14/2008

The first demonstration that a single adult stem cell can self-renew in a mammal was reported at the American Society for Cell Biology (ASCB) 48th Annual Meeting, Dec. 13-17, 2008 in San Francisco.

The transplanted adult stem cell and its differentiated descendants restored lost function to mice with hind limb muscle tissue damage.

The adult stem cells used in the study, conducted at Stanford University, were isolated from a mixed population of satellite cells in the skeletal muscle of mice.

The skeletal adult muscle stem cells (MusSC), which live just under the membrane that surrounds muscle fibers, normally respond to tissue damage by giving rise to progenitor cells that become myoblasts, fusing into myofibers to repair the tissue damage.

The scientists transplanted the MusSC into special immune-suppressed "nude" mice whose muscle satellite cells had been wiped out in a hind limb by irradiation.

The mice would only be able to repair injury if the transplanted MuSC "took." The scientists, Alessandra Sacco and Helen Blau, had genetically engineered the transplanted MusSC to express Pax7 and luciferase proteins. As a result, every transplanted cell glowed under ultraviolet light and was easy to trace.

"To be able to detect the presence of the cells by bioluminescence was really a breakthrough," says Blau. "It taught us so much more. We could see how the cells were responding, and really monitor their dynamics."

Through luminescent imaging as well as quantitative and kinetic analyses, Sacco and Blau tracked each transplanted stem cell as it rapidly proliferated and engrafted its progeny into the irradiated muscle tissue.

The scientists then injured the regenerated tissue, setting off massive waves of muscle cell growth and repair, and subsequently showed that the MuSC and descendents rescued the second animal's lost muscle healing function.

After isolating the luciferase-g
'/>"/>

Contact: Cathy Yarbrough
sciencematter@yahoo.com
858-243-1814
American Society for Cell Biology
Source:Eurekalert

Page: 1 2

Related biology news :

1. Einstein researchers develop technique to count messages made by single genes
2. Reprogrammable cell type depends on a single gene to keep its identity
3. Salk researchers successfully reprogram keratinocytes attached to a single hair
4. A single mechanism for hypertension, insulin resistance and immune suppression
5. Study shows single insecticide application can kill 3 cockroach generations
6. Researchers observe spontaneous ratcheting of single ribosome molecules
7. RevGenUK, a single-stop shop for use in functional genomics
8. Single-celled bacterium works 24-7
9. Rice scientists make breakthrough in single-molecule sensing
10. Blue-eyed humans have a single, common ancestor
11. Ebola virus disarmed by excising a single gene
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... Prenatal exposure to tetrachloroethylene (PCE) in drinking water ... abruption, according to a new study led by ... , The study, published in the journal ... 1,019 unexposed pregnancies among 1,766 women in Cape ... late 1960s to the early 1980s by the ...
(Date:9/30/2014)... -- Pollution in urban and farm runoff in Hawaii ... study finds. , The study, published Tuesday in ... nitrogen in the runoff ends up in algae that ... the animals, eyes, flippers and internal organs. , Scientists ... National Oceanic and Atmospheric Administration (NOAA) conducted the study ...
(Date:9/30/2014)... trouble getting pregnanteven with IVF? Here,s some hope: A ... The FASEB Journal , explains how ... known as PAWP, which induced embryo development in human ... embryo development by the sperm cell during fertilization. , ... a major paradigm shift in our understanding of human ...
Breaking Biology News(10 mins):Contaminated water linked to pregnancy complications, BU study finds 2Pollution linked to lethal sea turtle tumors 2Pollution linked to lethal sea turtle tumors 3Synthetic sperm protein raises the chance for successful in vitro fertilization 2
... may have to exercise even harder to get the benefits ... in the February issue of the journal Cell Metabolism, published ... a kind of metabolic boost in response to exercise is ... AMP-activated protein kinase (AMPK) slows down in the skeletal muscle ...
... Having a twin sister could put male saiga antelopes at ... shows that male twins with a sister are born lighter ... optimal size for males. The research also shows that saigas ... other similar species investing more in their offspring during ...
... how much they had already eaten -- in this case, ... who had no such environmental cues, finds a new Cornell ... eating at a table where leftover bones accumulated compared with ... than for women. , "The results suggest that people ...
Cached Biology News:Power-boosting signal in muscle declines with age 2The penalty of having a sister -- why sibling sex matters for male saiga antelopes 2The penalty of having a sister -- why sibling sex matters for male saiga antelopes 3
(Date:9/30/2014)... September 30, 2014 On September 11, Pharma ... Sherley in anticipation of a session that he will lead ... Summit,” scheduled for February 17-18, 2015 in London. In ... IQ asked Dr. Sherley to respond to several questions on ... challenges to evaluating stem cell toxicity, and future trends in ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 The ... IACRN), a non-profit organization based in Pittsburgh, ... Excellence and Safety (ACRES), a Massachusetts non-profit ... strategic alliance agreement, setting the stage for multiple ... enhance clinical research quality and safety through specialized ...
(Date:9/29/2014)... Sept. 29, 2014  Ten of the most ... cartilage repair, regeneration, allograft reconstruction and research and ... world gathered to discuss the future of cartilage ... Regenerative Medicine symposium, Cartilage Regeneration: State of ... Orthopedic Biosurgery, Minced Juvenile Allograft, and State of ...
(Date:9/29/2014)... are efficient but involve a range of resource ... seed) and pine resin and a clever recycling ... a highly interesting alternative. Their study will be ... . , ,We think our discovery can open ... the batteries of the future, says Daniel Brandell, ...
Breaking Biology Technology:Adult Stem Cell Technology Center, LLC Director James L. Sherley Discusses Stem Cell Toxicology 2Adult Stem Cell Technology Center, LLC Director James L. Sherley Discusses Stem Cell Toxicology 3Adult Stem Cell Technology Center, LLC Director James L. Sherley Discusses Stem Cell Toxicology 4IACRN and ACRES to Support Clinical Research Nurses 2IACRN and ACRES to Support Clinical Research Nurses 3World Renowned Experts Discuss Future Of Cartilage Regeneration 2World Renowned Experts Discuss Future Of Cartilage Regeneration 3Smart, eco-friendly new battery to solve problems 2
... Wisconsin-Madison , justified or otherwise, is that its academic ... has world-class scientists and well-developed mechanisms for turning their ... that commercialization takes place within a half-hour drive from ... the figures for per capita wages, unemployment and job ...
... subscribe to the theory that consumer need is what drives ... some proof. , ,In this case, the need is ... something to be desired, and the chief executive of a ... and broadband problems before. , ,Albert Chiozzi, who plans ...
... half of the nation's top 25 banks have joined an ... , a business unit of Metavante Corp ., the ... ,Twelve of the nation's largest banks now will be able ... physically convey them, a capability that was encouraged by the ...
Cached Biology Technology:Entrepreneurship grant will build strong foundation 2Entrepreneurship grant will build strong foundation 3Entrepreneurship grant will build strong foundation 4Company to operate in wireless Internet space 2
... 2D polyacrylamide gel electrophoresis (2D-PAGE) with biological ... core technology platform for proteomic analysis. 2D-PAGE ... samples limited only in the dynamic range ... proteins. Quantitative image based analysis of separated ...
... PLAU N-term Antibody This antibody is generated ... synthetic peptide selected within aa 50~150 of ... in PBS with 0.09% (W/V) sodium azide. ... G column and eluted out with both ...
... is a recombinant 177 amino acid polypeptide ... mature human MT1-MMP. Produced by activation ... purified from E. coli periplasm. SPECIFIC ... is = 140 mU/mg, where 1 U ...
... compact, lightweight, high current power supply capable of ... It is an excellent choice for PAGE, SDS-PAGE ... also ideal for all types of electroblotting applications.A ... automatically end a run and wil automatically accrue ...
Biology Products: