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Single GFP-expressing cell is basis of living laser device
Date:6/12/2011

nfirming that the GFP solution could amplify input energy into brief pulses of laser light, the researchers estimated the concentration of GFP required to produce the laser effect.

Using that information, their next step was to develop a line of mammalian cells expressing GFP at the required levels. The cellular laser was assembled by placing a single GFP-expressing cell with a diameter of from 15 to 20 millionths of a meter in a microcavity consisting of two highly reflective mirrors spaced 20 millionths of a meter apart. Not only did the cell-based device produce pulses of laser light as in the GFP solution experiment, the researchers also found that the spherical shape of the cell itself acted as a lens, refocusing the light and inducing emission of laser light at lower energy levels than required for the solution-based device. The cells used in the device survived the lasing process and were able to continue producing hundreds of pulses of laser light.

"While the individual laser pulses last for only a few nanoseconds, they are bright enough to be readily detected and appear to carry very useful information that may give us new ways to analyze the properties of large numbers of cells almost instantaneously," says Yun, who is an associate professor of Dermatology at Harvard Medical School. "And the ability to generate laser light from a biocompatible source placed inside a patient could be useful for photodynamic therapies, in which drugs are activated by the application of light, or novel forms of imaging."

Gather adds, "One of our long-term goals will be finding ways to bring optical communications and computing, currently done with inanimate electronic devices, into the realm of biotechnology. That could be particularly useful in projects requiring the interfacing of electronics with biological organisms. We also hope to be able to implant a structure equivalent to the mirrored chamber right into a cell, which would the
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Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2 3

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