Navigation Links
'Shotgun' method allows scientists to dissect cells' sugar coatings
Date:12/5/2010

Sugar molecules coat every cell in our bodies and play critical roles in development and disease, yet the components of these "glycans" have been difficult for scientists to study, because of their complexity.

Researchers at Emory University School of Medicine have adapted gene chip microarray technology to the study of glycans, with an approach they call "shotgun glycomics." The Emory team has developed a new chemical method for attaching a fluorescent dye to glycans purified from cells. The individual glycans are separated into tiny spots fixed to glass slides.

The approach is described in an article published this week in the journal Nature Methods.

"These slides separate and display all the glycans in the cell, so that we can test what sticks to them," says senior author David Smith, PhD, professor of biochemistry and director of the Glycomics Center at Emory University School of Medicine. "However, the structures of the glycans are unknown. This is why we use the word 'shotgun' to describe our quasi-random approach of studying them."

The research team was led by Smith, first author Xuezheng Song, PhD, assistant professor of biochemistry, and Richard Cummings, PhD, chair of the Department of Biochemistry and co-director of the Glycomics Center.

As a demonstration of the technique's utility, the team used it to identify a molecule recognized by self-reactive antibodies present in the blood of most patients with Lyme disease. Lyme disease is caused by infection with Borrelia bacteria after a tick bite, but severe cases have features of an autoimmune response, triggered by the immune system's reaction to the bacteria.

"Being able to analyze glycans in this way may lead to new diagnostics for human autoimmune disorders, and perhaps, therapies to cleanse the body of self-reactive antibodies or inhibit their pathological attack on cells," Cummings says.

Completely dissecting glycans' structur
'/>"/>

Contact: Holly Korschun
hkorsch@emory.edu
404-727-3990
Emory University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Commercial aquatic plants offer cost-effective method for treating wastewater
2. UC Riverside biochemists devise method for bypassing aluminum toxicity effects in plants
3. New lab manual focuses on essential methods for purifying and characterizing proteins
4. Deep biosphere research points to new methods for recovering petroleum
5. Fingerprinting method tracks mercury emissions from coal
6. Iowa State researcher develops new treatment method for canine eye diseases
7. New method provides panoramic view of protein-RNA interactions in living cells
8. Cold Spring Harbor Protocols features organ and cell culture methods
9. Engineer to present leak-proof method for carbon dioxide storage at international conference
10. Pitt researchers use fluorescence to develop method for detecting mercury in fish
11. New holographic method could be used for lab-on-a-chip technologies
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/11/2014)... Research awarded Michael F. Holick, PhD, MD, of Boston ... V. Avioli Award. Holick, a professor of medicine, physiology ... the understanding of vitamin D and its role in ... of the American Society for Bone and Mineral Research ... research. It is named for ASBMR,s first president and ...
(Date:7/11/2014)... USC Stem Cell scientists have set a "mouse TRAP" ... described by a recent study published in the ... line uses a technique called TRAP to extract cellular ... , Invented by scientists at the Rockefeller Institute for ... tag to the protein-making machinery, or ribosomes, of the ...
(Date:7/11/2014)... Shenzhen, China Researchers from Salk Institute for Biological ... time evaluated the safety and reliability of the ... a new method, TALEN-HDAdV, which could significantly increased ... (hiPSC). This study published online in Cell ... for stem cell-based gene therapy. , The combination ...
Breaking Biology News(10 mins):BUSM's Michael F. Holick receives American Society for Bone and Mineral Research award 2A new genome editing method brings the possibility of gene therapies closer to reality 2
... JUPITER, FL January 25, 2011 Embargoed by ... Scientists from the Florida campus of The Scripps Research ... an individual,s vulnerability to the addictive properties of nicotine. The ... study appeared January 30, 2011, in an advance, online issue ...
... of antibiotics that followed it, streptococcus bacteria have remained ... reason: the superb evolutionary skills of this pathogen to ... published this week in Science , scientists from ... genome sequencing to identify the precise steps in the ...
... of the journal Science , UC Davis expert ... also true, and explores what that might mean to ... vice-versa. A classic example of ecology influencing evolution ... In this species, larger beaks dominated the population after ...
Cached Biology News:Scripps Research scientists reveal key mechanism governing nicotine addiction 2Scripps Research scientists reveal key mechanism governing nicotine addiction 3New research traces evolutionary path of multidrug-resistant strep bacteria 2New research traces evolutionary path of multidrug-resistant strep bacteria 3
(Date:7/10/2014)... June 17, 2014 Using microscopic polymer light ... gases, researchers at MIT,s Quantum Photonics Laboratory have ... in the parts-per-billion range. Optical sensors are ideal ... high signal-to-noise ratio, compact, lightweight nature, and immunity ... sensors had been developed before, the MIT team ...
(Date:7/10/2014)... disorders has been extremely challenging because there are many ... to cause disease. Now investigators reporting in the July ... Stem Cell describe a strategy that may help ... risk factors or environmental exposures to affect the development ... variant that may predispose individuals to schizophrenia. , ...
(Date:7/10/2014)... The lithium-ion batteries that power our laptops and electric ... a single charge with the help of a sponge-like ... to replace the graphite traditionally used in one of ... times the energy storage capacity of graphite. A paper ... was published today in Nature Communications . , ...
(Date:7/10/2014)... have shelled out for crude 3-D glasses, polarized ... experience. These basic devices, used to trick the ... soon be rendered obsolete with the introduction of ... researchers. , TAU doctoral students Yuval Yifat, Michal ... holography based on nanoantennas that could be used ...
Breaking Biology Technology:Swell new sensors 2New strategy could uncover genes at the root of psychiatric illnesses 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3
... 0 clinical testing has been gaining acceptance and ... development process in the years to come. The ... and technology dependence is also expected to result ... in this sector.(Logo: http://www.newscom.com/cgi-bin/prnh/20081117/FSLOGO )"There is every ...
... Health Care Business Conference, an MBA-student organized event, will be held ... distinguished speakers and panelists from Healthcare Services, Investment, Pharma, Biotech, and ... ... 10, 2009 -- The 2009 Wharton Health Care Business Conference ...
... Feb. 10 DSM Biologics and Crucell N.V. (Euronext, ... today that they have entered into an agreement with ... Vendor Network. Under the terms of the agreement, Bioceros ... licensees of the PER.C6(R) cell line located in the ...
Cached Biology Technology:Frost & Sullivan Sees Microdosing as Compelling Advantage for Big Pharma 2Frost & Sullivan Sees Microdosing as Compelling Advantage for Big Pharma 314th Annual Wharton Health Care Business Conference to Feature CEOs from WebMD, McKesson, Humana and Given Imaging 2DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 2DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 3DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 4DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 5
...
Histone H2A Polyclonal Antibody 100 ug affinity purified rabbit polyclonal antibody. Detects ~14 kDa Histone H2A. It does not cross-react with other Histones. Reacts with human/mouse/rat. Performs w...
Contains L-glutamine...
... PhosphatidylEthanolamine-Binding Protein (PEBP) does not share ... inhibitors. PEBP is expressed in ... ovary, muscle, and stomach. One ... is exerting inhibitory activity against several ...
Biology Products: