Navigation Links
Shifting evolution into reverse promises cheaper, greener way to make new drugs
Date:3/23/2014

the development of life where early organisms were swimming in a primordial soup rich in organic material. In this environment, imagine that one of the species finds a use for the complex chemical compound A that gives it a competitive advantage. As a result, its population expands, consuming more and more compound A. Everything goes well until compound A becomes scarce. When that happens, individuals who develop an enzyme that allows them to substitute the still plentiful compound B for the scarce compound A gain a reproductive advantage and continue to grow while those who remain dependent on compound A die out. And so it goes until many generations later the survivors have developed multi-step chemical pathways to produce the molecules that they need to survive from the molecules available in their environment.

To test Bachmann's retro approach, the Vanderbilt chemists first identified the drug that they wanted to produce in this case didanosine, an anti-HIV drug sold under the trade names of Videx and Videx EC that is very costly to manufacture. Then they identified a similar "precursor" molecule that can be converted into didanosine when it is subject to a specific chemical transformation along with an enzyme capable of producing the type of transformation required.

Once they identified the enzyme, the researchers made use of the power of natural selection by making thousands of copies of the gene that makes the enzyme using a special copying technique that introduces random mutations.

The mutant genes were transferred into the gut bacteria E. coli in order to produce the mutant enzymes and placed into different "wells." After the cells were broken open and the contents mixed with the precursor compound, the amount of didanosine, in each well was measured. The researchers selected the enzyme that produced the greatest amount of the desired drug and then made enough of this optimized enzyme for the next step.

Next the resear
'/>"/>

Contact: David Salisbury
david.salisbury@vanderbilt.edu
615-343-6803
Vanderbilt University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Shifting the balance between good fat and bad fat
2. Atypical brain circuits may cause slower gaze shifting in infants who later develop autism
3. How a shape-shifting DNA-repair machine fights cancer
4. Leading evolutionary scientist to discuss how genome of bacteria has evolved
5. An evolutionary surprise
6. Ancient Egyptian cotton unveils secrets of domesticated crop evolution
7. Did climate change shape human evolution?
8. A University of Tennessee professors hypothesis may be game changer for evolutionary theory
9. Analysis of stickleback genome sequence catches evolution in action
10. Study shows unified process of evolution in bacteria and sexual eukaryotes
11. Rapid method of assembling new gene-editing tool could revolutionize genetic research
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/22/2015)... Washington , January 20, 2015 Investorideas.com, ... tech stocks, releases video from the CES 2015 (Consumer Electronics ... Apollo Robbins . Apollo shows how easily ... smart wallet, a product of NXT-ID, Inc. (NASDAQ: ...
(Date:1/22/2015)... Jan. 22, 2015 Infinisource has launched its new NXG ... model. The G2 sets a higher standard for collecting attendance ... management solution. With plug-and-play installation, touch screen interface and seamless ... a robust time collection solution for the small to mid-size ...
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ... the "iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 ... offering. http://photos.prnewswire.com/prnh/20130307/600769 ... Apple introduced the fingerprint reading feature with the iPhone ...
Breaking Biology News(10 mins):Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3Infinisource's NXG series sets new time clock standard 2iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 2
... biomedical engineer and colleagues have developed a method that, applied ... with increased breast cancer risk the pain and stress of ... lesion. The universal technology will give radiologists greater confidence ... The American Cancer Society recommends that women with certain ...
... an effort to increase understanding of the medical device ... of product development more effectively, researchers at Stanford University ... device development process. Published in the June ... , the model was constructed based on best-practice analysis ...
... Fla. -- The U.S. National Science Foundation (NSF) announced ... of building a multi-decadal time series of Agulhas Current ... Observing System. Led by Principal Investigator, Lisa Beal, ... Marine and Atmospheric Science, the international team will include ...
Cached Biology News:New MRI technique could mean fewer breast biopsies in high-risk women 2Stanford researchers publish comprehensive model for medical device development 2Stanford researchers publish comprehensive model for medical device development 3NSF provides $3.4 million to study climatically important Agulhas Current 2
(Date:1/22/2015)... The laboratory information management systems market is a rapidly ... advancements due to factors such as rising pressure to ... systems, and increasing government support for adoption of HCIT ... in the market focus on technological advancements to further ...
(Date:1/22/2015)... 22, 2015 zFlo Motion , a ... announce an exciting product from a new partner. STT-Systems ... over the past 15 years, with offerings in the optical-motion ... 3D IMU system (inertial measurement unit), iSen, is opening eyes ...
(Date:1/22/2015)... 22, 2015 Diagenode, Inc., a ... the Bioruptor® and complete solutions for epigenetics research, ... immunoprecipitation, alleviating the need for manual processing. The ... for ChIP of histones or transcription factors and ...
(Date:1/22/2015)... 22, 2015   GenoSpace , a precision medicine software company ... the broad use of genomic, imaging and other biomedical data ... Michelle Munson , CEO of Aspera, an IBM Company, ... - http://photos.prnewswire.com/prnh/20150122/170713 "We are pleased ...
Breaking Biology Technology:Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 43D Motion Capture Just Got Easy 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2GenoSpace Expands Board with Appointment of Michelle Munson 2
... - The Culex quinquefasciatus mosquito poses a ... of elephantiasis-causing worms and encephalitis-inducing viruses. An international team ... and the SIB Swiss Institute of Bioinformatics sequenced its ... articles published in today,s issue of Science, describe results ...
... - YM BioSciences Inc. (NYSE Amex: YMI, TSX:YM), ... its CYT387 JAK1/JAK2 inhibitor program demonstrating that CYT387 ... which may provide significant clinical advantages. CYT387 is ... study at Mayo Clinic in patients with myelofibrosis. ...
... Inc. - (OTC Bulletin Board: BHRT ) ... of autologous cell therapies for the treatment of cardiovascular ... commitment from Seaside National Bank and Trust for a ... Company,s loan with Bank of America. The loan is ...
Cached Biology Technology:Major disease-vector mosquito reveals the secrets of its immune system 2YM Biosciences To Present Poster On CYT387 At The European School Of Haematology International Conference 2YM Biosciences To Present Poster On CYT387 At The European School Of Haematology International Conference 3Bioheart, Inc. Announces Loan to be Assumed by Seaside 2Bioheart, Inc. Announces Loan to be Assumed by Seaside 3
... in your 35mm dish without transferring ... Chamber has separate openings for solution ... fluctuations of the liquid level in ... from entering the chamber. An adjustable ...
... Five cells (BTI-TN-5B1-4) derived from Trichoplusia ... shown to be capable of expressing ... proteins compared to other insect cells. ... features:- -Rapid doubling time as adherent cultures- Quick ...
... steadylite plus assay system offers ... sensitivity, Designed for batch-processing systems, ... higher density microplates such as ... free, Convenient storage conditions ( ...
... This kit consists of universal lysis buffer, ... primers for 100 25 l volume PCR ... of Gram negative or positive rod and ... Pneumoniae, Bacillus Cereus, Staphylococcus Aureus, Streptococcus Agalactiae, ...
Biology Products: