The scientists were also able to use pulses of light to steer the worms left or right. By stimulating neurons associated with the worm's reproductive system, they were even able to rouse the animal into secreting an egg.
Key to the CoLBeRT system is a tracking microscope recording the motion of a swimming or crawling worm, paired with image processing software that can quickly estimate the location of individual neurons and instruct a digital micromirror device to illuminate targeted cells. Because cells in an unrestrained worm represent a rapidly moving target, the system can capture 50 frames per second and attain spatial resolution of just 30 microns.
"This development should have profound consequences in systems neuroscience as a new tool to probe nervous system activity and behavior, as well as in bioengineering and biorobotics," Samuel says. "Our laboratory has been pioneering new optical methods to study the nervous system, and this is the latest, and perhaps our greatest, invention."
|Contact: Steve Bradt|