Navigation Links
Sequencing tracks animal-to-human transmission of bacterial pathogens

HEIDELBERG, 25 March 2013 Researchers have used whole genome sequencing to reveal if drug-resistant bacteria are transmitted from animals to humans in two disease outbreaks that occurred on different farms in Denmark. The results, which are published today in EMBO Molecular Medicine, confirm animal-to-human transmission of methicillin-resistant Staphylococcus aureus (MRSA), a disease-causing bacterium that carries the recently described mecC gene. The mecC gene is responsible for resistance to the penicillin-like antibiotic methicillin.

Drug-resistant bacterial infections pose a significant challenge to public health and may have severe and sometimes fatal consequences. As the costs of whole genome sequencing methods continue to plummet and the speed of analysis increases, it becomes increasingly attractive for scientists to use whole genome sequencing to answer disease-related questions.

"We used whole genome sequencing to see if we could determine if the two disease outbreaks were caused by the same bacterium and to investigate if the pathogens were transmitted from animal to humans or the other way around," remarked Mark Holmes, from the University of Cambridge and the senior author on the paper. "At first glance, it seems reasonable to expect the same pathogen to be the source of the two outbreaks at the two geographically close locations. By looking at the single differences in nucleotides or SNPs in the DNA sequences of each isolate, it became obvious that two different strains of bacteria were responsible for the two disease outbreaks. In one case, the results also clearly showed that the most likely direction of transmission was from animal to human."

Methicillin-resistant S. aureus can lead to debilitating skin and soft tissue infections, bacteremia, pneumonia and endocarditis. The researchers used an Illumina HiSeq sequencing system to take a close look at the nucleotide sequence of each pathogen. By comparing single difference in nucleotides in the two sequences (single nucleotide polymorphisms) they were able to reach conclusions about the identity of the pathogens and the routes of infection.

The researchers emphasize that while whole genome sequencing cannot replace other more traditional types of diseases analysis it can greatly increase the ability of scientists to distinguish between different pathogens as the cause of disease.

"Our findings demonstrate that the MRSA strains we studied are capable of transmission between animals and humans, which highlights the role of livestock as a potential reservoir of antibiotic-resistant bacteria," remarked Ewan Harrison, one of the lead authors of the study.


Contact: Barry Whyte
European Molecular Biology Organization

Related biology news :

1. Powerful sequencing technology decodes DNA folding pattern
2. Sequencing works in clinical setting to help -- finally -- get a diagnosis
3. The sequencing of the tomato genome
4. DNA Sequencing: Emerging Technologies and Applications
5. Next-generation sequencing technology opens doors to discoveries
6. University of North Texas Health Science Center Advances Forensic Research by Investing in Semiconductor DNA Sequencing Technology
7. Exome sequencing gives cheaper, faster diagnosis in heterogeneous disease
8. An error-eliminating fix overcomes big problem in 3rd-gen genome sequencing
9. Exome sequencing of health condition extremes can reveal susceptibility genes
10. New genomic sequencing method enables smarter anaysis of individual cells
11. US-Russian collaboration develops new method for sequencing dark matter of life from a single cell
Post Your Comments:
(Date:10/29/2015)... 2015 NXTD ) ("NXT-ID" ... on the growing mobile commerce market and creator ... a leading marketplace to discover and buy innovative ... wallet on StackSocial for this holiday season.   ... "Company"), a biometric authentication company focused on the ...
(Date:10/27/2015)... , Oct. 27, 2015 In the ... issues of concern for various industry verticals such as ... due to the growing demand for secure & simplified ... various ,sectors, such as hacking of bank accounts, misuse ... electronic equipment such as PC,s, laptops, and smartphones are ...
(Date:10/27/2015)... 2015 Synaptics Inc. (NASDAQ: SYNA ), the ... has adopted the Synaptics ® ClearPad ® ... its newest flagship smartphones, the Nexus 5X by LG ... --> --> Synaptics works closely ... collaboration in the joint development of next generation technologies. ...
Breaking Biology News(10 mins):
(Date:11/26/2015)... , November 26, 2015 /PRNewswire/ ... --> Accutest Research Laboratories, ... Contract Research Organization (CRO), has formed ... Cancer Center - Temple Health for ... ,     (Photo: ) , ...
(Date:11/25/2015)... Studies reveal the differences in species of bacteria ... for more effective treatment for one of the most commonly ... --> --> Gum disease is one ... relatively little was understood about the bacteria associated with it ... researchers from the WALTHAM Centre for Pet Nutrition together with ...
(Date:11/25/2015)... , Nov. 25, 2015 Orexigen® Therapeutics, Inc. ... participate in a fireside chat discussion at the Piper ... York . The discussion is scheduled for Wednesday, ... .  A replay will be available for ... Stilwell  , Julie NormartVP, Corporate Communications and Business ...
(Date:11/24/2015)... Cepheid (NASDAQ: CPHD ) today announced ... conference, and invited investors to participate via webcast. ... December 1, 2015 at 11.00 a.m. Eastern Time ... December 1, 2015 at 11.00 a.m. Eastern Time ... York, NY      Tuesday, December 1, 2015 at ...
Breaking Biology Technology: