Navigation Links
Sequencing hundreds of chloroplast genomes now possible
Date:1/31/2013

Researchers at the University of Florida and Oberlin College have developed a sequencing method that will allow potentially hundreds of plant chloroplast genomes to be sequenced at once, facilitating studies of molecular biology and evolution in plants.

The chloroplast is the compartment within the plant cell that is responsible for photosynthesis and hence provides all of the sugar that a plant needs to grow and survive. The chloroplast is unusual in containing its own DNA genome, separate from the larger and dominant genome that is located in every cell's nucleus.

Chloroplast DNA sequences are widely used by plant biologists in genetic engineering and in reconstructing evolutionary relationships among plants. Until recently, though, chloroplast genome sequencing was a costly and time-intensive endeavor, limiting its utility for plant evolutionary and molecular biologists. Instead, most researchers have been limited to sequencing a small portion of the chloroplast genome, which in many cases is insufficient for determining evolutionary relationships, especially in plant groups that are evolutionarily young.

In contrast, complete chloroplast genome sequences harbor enough information to reconstruct both recent and ancient diversifications. New DNA sequencing technologies, termed "next-generation" sequencers, have made it considerably cheaper and easier to sequence complete chloroplast genomes. While current methods using next-generation sequencers allow up to 48 chloroplast genomes to be sequenced at one time, the new method will allow potentially hundreds of flowering plant chloroplast genomes to be sequenced at once, significantly reducing the per-sample cost of chloroplast genome sequencing.

This new method, reported in the February issue of Applications in Plant Sciences (available for free viewing as part of the February Issue in Progress at http://www.bioone.org/doi/pdf/10.3732/apps.1200497), relies on efficient separation of chloroplast DNA from other DNA in the cell using short DNA "baits" that were designed from chloroplast genomes that have already been sequenced. These molecular baits effectively concentrate the chloroplast DNA before sequencing (a process termed "targeted enrichment"), dramatically increasing the number of samples that can be sequenced at once.

Greg Stull, a graduate student at the University of Florida and lead author of the study, summarizes the versatility of the new system: "With this method, it should be possible for researchers to cheaply sequence hundreds of chloroplast genomes for any flowering plant group of interest."

The method was specifically designed by the authors of the study such that almost any flowering plant chloroplast genome can be sequenced, regardless of species. Flowering plants represent the largest (~300,000 species) and most ecologically dominant group of land plants, and include all major crop plants.


'/>"/>
Contact: Beth Parada
bparada@botany.org
American Journal of Botany
Source:Eurekalert

Related biology news :

1. Researchers develop tool to evaluate genome sequencing method
2. Genetic sequencing breakthrough to aid treatment for congenital hyperinsulinism
3. Gene sequencing project identifies abnormal gene that launches rare childhood leukemia
4. Duke Medicine news -- Genome sequencing of Burkitt Lymphoma reveals unique mutation
5. Markets for PCR, DNA Microarray, DNA Sequencing, Mass Spectrometry and Flow Cytometry to Exceed $50 billion by 2015
6. NIH-funded genetic sequencing tool speeds drug discovery, disease diagnostics
7. Columbia researchers report novel approach for single molecule electronic DNA sequencing
8. BGI Tech develops whole exome sequencing analysis of FFPE DNA samples to boost biomedicine
9. New NIH/NHGRI grants to harness nanoscale technologies to cut DNA sequencing costs
10. Prenatal whole genome sequencing: Just because we can, should we?
11. US-Russian collaboration develops new method for sequencing dark matter of life from a single cell
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/24/2017)... 2017 Janice Kephart , former ... Strategy Partners, LLP (IdSP) , today issues the ... Trump,s March 6, 2017 Executive Order: Protecting ... can be instilled with greater confidence, enabling the ... refugee applications are suspended by until at least ...
(Date:4/13/2017)... , April 13, 2017 According to a new ... Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, ... IAM Market is expected to grow from USD 14.30 Billion in 2017 ... (CAGR) of 17.3%. ... MarketsandMarkets Logo ...
(Date:4/6/2017)... Forecasts by Product Type (EAC), Biometrics, Card-Based ... & Logistics, Government & Public Sector, Utilities / Energy ... Nuclear Power), Industrial, Retail, Business Organisation (BFSI), Hospitality & ... for a definitive report on the $27.9bn Access Control ... ...
Breaking Biology News(10 mins):
(Date:5/22/2017)... ... May 22, 2017 , ... Stratevi, a boutique firm that partners with healthcare ... has opened an office in downtown Boston at 745 Atlantic Ave. , “We ... to generate evidence on the value they provide, not just to patients, but also ...
(Date:5/21/2017)... ... May 19, 2017 , ... Ovation Fertility scientists’ work is ... of Bioanalysts (AAB) and the College of Reproductive Biology (CRB) today and Saturday ... to excellence in clinical laboratory services and regulations. , “We are pleased ...
(Date:5/19/2017)... ... May 19, 2017 , ... The University City ... with technologies ripe for commercialization, and who are affiliated with the 21 partner ... submit proposals. QED, now in its tenth round, is the first multi-institutional proof-of-concept ...
(Date:5/18/2017)... ... 16, 2017 , ... Clinical Supplies Management (“CSM”), a Great Point Partners II ... grow. CSM has doubled in size over the past six months with the ... , Roger Gasper joins CSM as Chief Financial Officer. Roger has over 25 ...
Breaking Biology Technology: