Navigation Links
Sensor gives valuable data for neurological diseases and treatments

WEST LAFAYETTE, Ind. - A new biosensor developed at Purdue University can measure whether neurons are performing correctly when communicating with each other, giving researchers a tool to test the effectiveness of new epilepsy or seizure treatments.

Marshall Porterfield, an associate professor of agricultural and biological engineering and biomedical engineering, postdoctoral researcher Eric McLamore, and graduate student Subhashree Mohanty developed the self-referencing glutamate biosensor to measure real-time glutamate flux of neural cells in a living organism. The nanosensor not only measures glutamate around neural cells, it can tell how those cells are releasing or taking up glutamate, a key to those cells' health and activity.

"Before we did this, people were only getting at glutamate indirectly or through huge, invasive probes," said Porterfield, whose research was published in the early online version of the Journal of Neuroscience Methods. "With this sensor, we can 'listen' to glutamate signaling from the cells."

The firing of neurons is involved in every action or movement in a human body. Neurons work electrically, but ultimately communicate with each other through chemical neurotransmitters such as glutamate. One neuron will release glutamate to convey information to the next neuron's cell receptors.

Once the message is delivered, neurons are supposed to reabsorb or clear out the glutamate signal. It is believed that when neurons release too much or too little glutamate and are not able to clear it properly, people are prone to neurological diseases.

Jenna Rickus, an associate professor of agricultural and biological engineering and biomedical engineering who oversaw the study's neurological aspects, said researchers need more information about how neurons work to create more effective treatments for neurological disorders.

"Understanding neurotransmitter dynamics has implications for almost all normal and pathological brain function," Rickus said. "The reason we don't have good information is because we haven't had a good measurement tool before."

Porterfield and McLamore's sensor exploits conductive carbon nanotubes and is only 2 micrometers in diameter, or about 50 times smaller than the diameter of a human hair. They also use an enzyme, called glutamate oxidase, on the end of the probe that reacts with glutamate to create hydrogen peroxide. The carbon nanotubes enhance the conductivity of the hydrogen peroxide, and a computer can calculate the movement of glutamate relative to the cell surface.

The sensor oscillates and samples the concentration activities of glutamate at various positions relative to the neurons in culture. Those measurements at different distances can tell researchers whether the glutamate is flowing back toward the neurons or dissipating in many directions.

Current sensor technology allows for sensing in vitro, but those probes are large and invasive, Porterfield said, and they don't measure the movement of the chemicals.

McLamore said the sensor also is valuable because it is able to hone in on only glutamate using just one probe and custom software that filters out variations in the signals that are read, which removes signal noise due to other compounds.

"There are many compounds present near the neurons which can potentially create noise, but this sensor should be selective for one compound. We filter out all of the background noise," McLamore said. "It's the same thing modern hearing aids do. They're filtering out ambient noises, and that's the same thing you get when you oscillate a biosensor."

The sensor also could be adapted to measure other chemicals by changing the enzyme used on its tip.

Rickus said the sensor's versatility would be valuable for understanding the effects of therapies for epilepsy, Parkinson's disease, damage caused by chemotherapy, memory loss and many other conditions. The sensor will give valuable data on how damaged neurons function and how drugs or therapies affect those cells.

Porterfield said the next step is to make small improvements to the sensor and adapt it to use other enzymes. The Office of Naval Research funded the research.


Contact: Brian Wallheimer
Purdue University

Related biology news :

1. Louisiana Tech researchers investigate tracking, sensors to assist Air Force
2. UCLA/VA partners with ASU to advance biosensor technology for urinary tract infections
3. New microsensor measures volatile organic compounds in water and air on-site
4. New field-deployable biosensor detects avian influenza virus in minutes instead of days
5. bioMETRX, Inc. Announces Integration of AuthenTec Fingerprint Sensor in smartTOUCH(TM) Product Line
6. MIT gas sensor is tiny, quick
7. Material Technologies Holds First Electrochemical Fatigue Sensor Training for Private Inspection Firms
8. Carnegie Mellon scientists develop fluorescent proteins for live cell imaging, biosensor design
9. Atmel Receives Frost & Sullivans Technology Innovation Award for FingerChip(R) Biometric Sensor
10. New cell-based sensors sniff out danger like bloodhounds
11. New gas sensors for monitoring carbon dioxide sinks
Post Your Comments:
(Date:4/5/2017)...  The Allen Institute for Cell Science today announces ... portal and dynamic digital window into the human cell. ... application of deep learning to create predictive models of ... a growing suite of powerful tools. The Allen Cell ... publicly available resources created and shared by the Allen ...
(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
(Date:3/29/2017)... , March 29, 2017  higi, the health IT ... North America , today announced a ... the acquisition of EveryMove. The new investment and acquisition ... of tools to transform population health activities through the ... data. higi collects and secures data today ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... Personal eye wash is a basic first aid supply for ... time. So which eye do you rinse first if a dangerous substance enters both eyes? ... Eye Wash with its unique dual eye piece. , “Whether its dirt and debris, ...
(Date:10/11/2017)... ... , ... Disappearing forests and increased emissions are the main causes of the ... Especially those living in larger cities are affected by air pollution related diseases. , ... pollution-affected countries globally - decided to take action. , “I knew I had to ...
(Date:10/10/2017)... ... October 10, 2017 , ... For the ... won a US2020 STEM Mentoring Award. Representatives of the FirstHand program travelled to ... Experience from US2020. , US2020’s mission is to change the trajectory of STEM ...
(Date:10/10/2017)... , ... October 10, 2017 , ... ... of 13 prestigious awards honoring scientists who have made outstanding ... a scheduled symposium during Pittcon 2018, the world’s leading conference and exposition for ...
Breaking Biology Technology: