Navigation Links
Seeing previously invisible molecules for the first time
Date:10/23/2009

A team of Harvard chemists led by X. Sunney Xie has developed a new microscopic technique for seeing, in color, molecules with undetectable fluorescence. The room-temperature technique allows researchers to identify previously unseen molecules in living organisms and offers broad applications in biomedical imaging and research.

The scientists' results are published in the Oct. 22 issue of Nature. Partial funding for the project was provided by the National Science Foundation (NSF).

Fluorescence is a phenomenon in which an electron in a molecule absorbs energy from light and moves to a higher energy level or excited state. The energy of the light is contained in a unit called a photon.

After a very brief stay at the excited state, the electron returns to its previous energy level, or ground state, by emitting a new photon. The energy of the released photon is discharged in wavelengths of detectable visible light lasting only a few billionths of a second.

Many biologically important colored molecules such as hemoglobin--an oxygen-transport protein in red blood cells--absorb light but do not fluoresce. Instead, the electrons in these molecules release their additional but transient energy by converting it to heat.

"Since these molecules do not fluoresce, they have literally been overlooked by modern optical microscopes," Xie said.

To detect non-fluorescent molecules in biological systems, Xie and his team developed a new type of microscopy based on stimulated emission.

Stimulated emission was first described by Albert Einstein in 1917, and was the basis for today's lasers. In a nutshell, it is a process by which an excited-state electron, perturbed by a photon having the correct energy, drops to its ground state producing an additional photon.

Xie's new microscopic technique generates and records a stimulated emission signal by using two carefully timed input and output pulse trains. In
'/>"/>

Contact: Jennifer A. Grasswick
jgrasswi@nsf.gov
703-292-4972
National Science Foundation
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Seeing the tree from the forest: Predicting the future of plant communities
2. Seeing stem cells helps in fight against peripheral arterial disease
3. Seeing through the skin
4. Crystal (eye) ball: Study says visual system equipped with future seeing powers
5. Seeing Alzheimers amyloids
6. Genome duplication responsible for more plant species than previously thought
7. Oscar Pistorius: Previously confidential study results released on amputee sprinter
8. Lower increases in global temps could lead to greater impacts than previously thought, study finds
9. Evolutionary process more detailed than previously believed, study shows
10. Nations that sow food crops for biofuels may reap less than previously thought
11. Whispering bats are 100 times louder than previously thought
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/17/2014)... inhibits RSV infection , Respiratory syncytial virus ... tract infections, generating life-threating illness in very young ... are limited. RSV enters host cells through the ... fusogenic bundle. Small interfering peptides that prevent bundle ... peptides are highly susceptible to degradation. In this ...
(Date:4/17/2014)... Professor Marc Andr Gauthier and Professor Luca Razzari ... each been awarded large grants from the John ... for Innovation (CFI) for the acquisition of state-of-the-art ... be added matching grants from the Ministre de ... et de la Technologie (MESRST). These new laboratories ...
(Date:4/17/2014)... marrow need to produce hydrogen sulfide in order to ... new study from the Center for Craniofacial Molecular Biology ... , Professor Songtao Shi, principal investigator on the project, ... cells governs the flow of calcium ions. The essential ... in osteogenesis, or the creation of new bone tissue, ...
Breaking Biology News(10 mins):JCI online ahead of print table of contents for April 17, 2014 2JCI online ahead of print table of contents for April 17, 2014 3JCI online ahead of print table of contents for April 17, 2014 4JCI online ahead of print table of contents for April 17, 2014 5JCI online ahead of print table of contents for April 17, 2014 6New state-of-the-art biotech and nanotech equipment for INRS 2
... 16, 2011The American Association for the Advancement of ... Srivastava, MD, a Fellow for his efforts to ... directs cardiac and stem cell research at Gladstone, ... research efforts are focused in the areas of ...
... (OGI) has awarded two research facilities with $10,000 each ... the work of Ontario researchers., ,The two recipients ... The Hospital for Sick Children (SickKids) in Toronto and ... StemCore Laboratories based at the Ottawa Hospital Research Institute. ...
... animal, from a blue whale to a human being, ... the cells in its body will die without reproducing; ... the next generation. How could the extreme degree ... aren,t all creatures unicellular individualists determined to pass on ...
Cached Biology News:Gladstone scientist Deepak Srivastava named fellow of the AAAS 22 research facilities awarded technology seeding grants 2Close family ties keep microbial cheaters in check, study finds 2Close family ties keep microbial cheaters in check, study finds 3Close family ties keep microbial cheaters in check, study finds 4Close family ties keep microbial cheaters in check, study finds 5
(Date:1/14/2014)... 2014 The largest international professional organization of ... therapeutic derivatives thereof has endorsed an educational program ... the challenges of adulterated herb and botanical products. ... The Society for Medicinal Plant and Natural ...
(Date:1/14/2014)... Boston, MA (PRWEB) January 14, 2014 iLab ... Michelle Detwiler as the new Director of Product Strategy. In ... as well as iLab sub-teams to guide in the development ... that iLab provides the maximum possible benefit to the scientific ...
(Date:1/14/2014)... 2014  RXi Pharmaceuticals Corporation (OTCQX: RXII), a biotechnology ... therapies addressing major unmet medical needs using RNA-targeted ... of Allowance from the United States Patent and ... (sd-rxRNA®), for the treatment of fibrosis. The patent ...
(Date:1/14/2014)... 2014   Kinaxis ®, provider of RapidResponse ®, a ... operations planning ( S&OP ) service, is proud to be ... will be held at the Hilton San Diego Bayfront, January ... Elisabeth Kaszas , Director of Supply Chain at Amgen, ...
Breaking Biology Technology:World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... BALTIMORE, March 24, 2011 Champions Biotechnology, Inc. ... a technology collaboration agreement with Cephalon, Inc. in ... on two proprietary chemical compounds, CEP-32496, an inhibitor ... ALK-FAK inhibitor, provided by Cephalon to determine the ...
... Calif., March 24, 2011 ValGenesis, Inc., an ... solutions, today announced the availability of its industry-proven ... (SaaS) offering hosted on MUSA,s Validated Cloud. ... Lifecycle Management solution accessible via the Internet through ...
... Rules-Based Medicine, Inc. (RBM), a leading provider of innovative ... OncologyMAP ® , a powerful tool developed at the ... Institute. Through quantitative measurement of 101 cancer-related proteins, OncologyMAP ... on biological pathways that could hold the key to ...
Cached Biology Technology:Champions Announces Technology Collaboration Utilizing Champions' Tumorgraft™ Technology Platform for Oncology Drug Development 2Champions Announces Technology Collaboration Utilizing Champions' Tumorgraft™ Technology Platform for Oncology Drug Development 3Announcing ValGenesis On-Demand SaaS Solution 2Announcing ValGenesis On-Demand SaaS Solution 3Rules-Based Medicine Launches OncologyMAP® to Advance Cancer-Related Drug and Diagnostic Development 2
... protein assay kit I provides reagents and a ... presence of reducing agents and detergents. The RC ... kit includes reagents and standards sufficient for 500 ... ml RC reagent II, 250 ml alkaline copper ...
5X StabilCoat Immunoassay Stabilizer is a 5X concentrate solution of StabilCoat Immunoassay Stabilizer. The shelf-life of 5X StabilCoat Immunoassay Stabilizer is 2 years when stored at 4C. Product ...
... three-color reagent kit for determining the absolute ... blood samples, mobilized peripheral blood samples, and ... a total nucleated cell count and a ... is designed for use in the BD ...
... Rhodopsin is a glycoprotein which is ... and reptiles. The rhodopsin protein is present ... Immunogen: Membrane preparation from ... This antibody specifically labels the axons andsynaptic ...
Biology Products: