Navigation Links
Secrets of a precision protein machine
Date:4/15/2011

le unpaired nucleotide. The other double-strand binding site is at the wrist of the boxing glove, near the 5' flap.

Two other sites hold the template strand opposite the open junction and, crucially, at the crease between thumb and forefinger, where the 5' flap will be cut.

In human FEN1, the arch between the thumb and the rest of the glove is indeed capped, although this is not true for other members of FEN1's family. In FEN1 the 5' flap is forced to thread through the arch, which is too narrow for double-strand DNA to enter and thus selects for the single-stranded flap.

The flap is cut at a place called the "scissile phosphate." Phosphates (and sugars) are like the vertebrae of a single strand's backbone, to which the bases are anchored (a phosphate plus a base makes one nucleotide). The flap is held firmly in the right place for cutting because the nucleotide with the scissile phosphate, plus its neighboring nucleotide, are initially base-paired to the template strand. The bend in the template strand opens these pairs and lines up the target nucleotides near two metal ions, which catalyze the hydrolysis the clipping of the flap at the precise scissile-phosphate location.

"The structure was unexpected, but it made clear how FEN1 works," says Tsutakawa. "At the same time it explains how the superfamily of similar endonucleases can operate in such different situations."

Motion and precision

"How does FEN1 contribute to recombination and repair accurately, without mistakes?" Tainer asks. "We see how moving the template will position the 5' flap for precise cutting. Incision is equally precise. FEN1 cuts it where it knows the strand has already been accurately paired to the template, leaving just one unpaired base, which can then pair with the unpaired 3′ overhang."

Tsutakawa summarizes the findings: "FEN1 grasps the template of the double-strand DNA where the junction is be
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Breaking the mucus barrier unveils cancer cell secrets
2. Uncovering the genome secrets of the Blackleg fungus
3. A pesky bacterial slime reveals its survival secrets
4. Unlocking the secrets of a plants light sensitivity
5. Unlocking the secrets of a plant’s light sensitivity
6. Discovery of the secrets that enable plants near Chernobyl to shrug off radiation
7. Air flows in mechanical device reveal secrets of speech pathology
8. Pigs reveal secrets: New research shines light on Quebec industry
9. Looking for secrets to drug addiction in our blood
10. Neutrons helping ORNL researchers unlock secrets to cheaper ethanol
11. Ancient brew masters tapped antibiotic secrets
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Secrets of a precision protein machine
(Date:7/25/2014)... molecular testing panel developed at UPMC greatly increases ... for patients with thyroid nodules and cancer, report ... (UPCI), partner with UPMC CancerCenter., The test, available ... diagnostic testing agencies, improved the chances of patients ... according to the study published this month in ...
(Date:7/25/2014)... July 25, 2014 Biometrics industry ... turning to digital technology for online & commerce payments ... Inc. (NASDAQ: GOOG ), LifeLock Inc. (NYSE: ... V ), Apple Inc. (NASDAQ: AAPL ) ... (OTCQB: NXTD) a biometric authentication company focused on the ...
(Date:7/24/2014)... loss leads to conflict among people around the world, ... (WCS) Health & Ecosystems: Analysis of Linkages (HEAL) Program ... to tackle global biodiversity decline. , The harvest of ... people and provides protein for more than a billion ... surprise that today,s unprecedented loss of wildlife, is bringing ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 2Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 3Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 4Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 5Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 6New study draws links between wildlife loss and social conflicts 2
... diverse, densely packed human population, head for New York,s ... people, then Southwestern Florida is your best bet. This ... Strand Preserve State Park not far from the Everglades ... a census led by Robert Lcking, collections manager and ...
... potato salad? Young adults who frequently attend religious activities ... middle age as young adults with no religious involvement, ... first longitudinal study to examine the development of obesity ... "We don,t know why frequent religious participation is associated ...
... Gentel Biosciences, a leader in proteomics tools, and Van ... today the granting of U.S. Patent 7,838,634 for a new ... on the surface of an antibody array slide. Gentel ... 2006. According to Brian H. Haab, Ph.D., head of ...
Cached Biology News:Study finds remarkable diversity of lichen species in Florida state park 2Study finds remarkable diversity of lichen species in Florida state park 3Religious young adults become obese by middle age 2Religious young adults become obese by middle age 3New technology to improve early detection and prognosis of cancer 2
(Date:7/25/2014)... 25, 2014 Sinovac Biotech Ltd. (Nasdaq: ... China , today announced that it will release ... 30, 2014, after market close on Thursday, August 14, 2014 ... the market opening on Friday, August 15, 2014, at 8:00 ... Standard Time) to review the Company,s financial results and provide ...
(Date:7/24/2014)... , July 24, 2014  Now available is a ... Rehealth Regenerative Therapies , located in ... physically active people a new health option: stem ... of age, countless patients suffer from joint and muscle ... as muscle tears, torn rotator cuff, tennis elbow, and ...
(Date:7/24/2014)... leader Yung-Eun Sung has announced that they have ... graphenes which can be applied as high performance ... Sung is both a group leader at the ... Science* (IBS) and a professor at the Seoul ... with regards to the development of relative simplicity, ...
(Date:7/24/2014)... Research and Markets has announced the addition ... report to their offering.  http://photos.prnewswire.com/prnh/20130307/600769 ... vitro growth of plant or animal cells for several ... Cell culture is a crucial step in tissue engineering ... consumables are necessary for the in vitro production of ...
Breaking Biology Technology:Sinovac to Host Conference Call to Report Second Quarter 2014 Unaudited Financial Results 2Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3Global Cell Culture Market 2014-2018: Key Vendors are Becton, Dickinson and Co, EMD, GE Healthcare, Lonza Group, Sigma Aldrich and Thermo Fisher Scientific 2
... - Dan Rooney knows soil. He also knows ... after starting his Madison-based company, his ideas about turning ... ,"There is information about location everywhere," said Rooney, founder ... , ,Combining that information with the growing power of ...
... Eric Schmidt, then the CTO of Sun Microsystems, gave ... language called Java. A thoroughly modern development platform for ... Java promised to ease programming headaches, the biggest of ... another. , ,Today, we can debate whether Java ...
... Wis. - Craig Schiefelbein believes in reincarnation - in ... - and still counting - reiterations of his company, ... its 20 th anniversary with a very important streak ... is a rare breed - a memory chip broker that ...
Cached Biology Technology:Global information firm reaches diverse markets 2Global information firm reaches diverse markets 3Global information firm reaches diverse markets 4Java's undeniable impact on the Web 2Java's undeniable impact on the Web 3PDS survives with technological reinvention 2PDS survives with technological reinvention 3PDS survives with technological reinvention 4PDS survives with technological reinvention 5
... PowerPac 3000 power supply is the ... for isoelectric focusing, DNA sequencing, isotachophoresis, ... western blotting, and submerged gel nucleic ... powre, the PowerPac 3000 power supply ...
... wide mini ReadySub-Cell GT cell and PowerPac basic ... electrophoresis of nucleic acids in agarose gels. The ... lid with cables and electrodes, and leveling bubble. ... V, provides output of 10-300 V, 4-400 mA, ...
... and you reduce the chance of error. ... 8000. Developed using Beckman Coulters extensive experience ... electrophoresis technology, the CEQ 8000 is a ... automatically fills the capillary array with a ...
... CZE 2000 Capillary Electrophoresis System uses ... protein electrophoresis (SPE), urine protein electrophoresis ... and accurate analysis. The system is ... preparation and no staining to process ...
Biology Products: