Navigation Links
Secrets of a precision protein machine
Date:4/15/2011

"pseudo-DNA" to whose backbone the polymerase can start adding DNA nucleotides to match with the template. The polymerase keeps on adding nucleotides until it runs into the RNA primer from the previous start.

This doesn't happen often on the so-called leading strand of the unwound DNA, where the polymerase usually proceeds smoothly, as if on a one-way street. The one-way direction, named for the chemical composition of the nucleotides at opposite ends of a DNA strand, is 5' to 3' (five-prime to three-prime).

On the lagging strand, however, the one-way street runs in the opposite direction, so replication has to be done in many little discrete fragments assembled "backwards," each started by an RNA primer. Called Okazaki fragments, these are only about 100 nucleotides long in humans, and some 50 million of them are added to the lagging strand during a human cell's replication.

When polymerase runs into the RNA primer of a previous fragment it peels it away like a chisel, leaving a long tail or "flap" of excess single-strand DNA at the 5' end. This flap must be clipped off before the new fragment can be joined failure to do this accurately would leave a gap or overlap that could cause a genetic mutation or rearrangement resulting in damaged chromosomes. FEN1 cuts off the flap and precisely prepares it for joining to the newer fragment, which is also left with a tiny flap, known as the 3' overhang.

Just how does FEN1 do the job? Tainer notes that many important structural features of human FEN1 were known from studies in 2007 by Shigeru Sakurai of Japan's Nara Institute of Science and Technology and from work on related FEN from microorganisms known as archaea. These studies were done without FEN1 acting on DNA, however, and many questions remained.

For example, FEN1 was long thought to move into position by sliding down the 5' flap to the incision site, like a bead on a string. The 5' flap passes through an opening
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Breaking the mucus barrier unveils cancer cell secrets
2. Uncovering the genome secrets of the Blackleg fungus
3. A pesky bacterial slime reveals its survival secrets
4. Unlocking the secrets of a plants light sensitivity
5. Unlocking the secrets of a plant’s light sensitivity
6. Discovery of the secrets that enable plants near Chernobyl to shrug off radiation
7. Air flows in mechanical device reveal secrets of speech pathology
8. Pigs reveal secrets: New research shines light on Quebec industry
9. Looking for secrets to drug addiction in our blood
10. Neutrons helping ORNL researchers unlock secrets to cheaper ethanol
11. Ancient brew masters tapped antibiotic secrets
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Secrets of a precision protein machine
(Date:7/9/2014)... the oils produced by algae may offer a low-cost ... optimization of cost effective strategies for large-scale algae cultivation, ... Energy has developed an innovative solution to the challenge ... in Industrial Biotechnology , a peer-reviewed journal from ... on the Industrial Biotechnology website . ...
(Date:7/9/2014)... Ill. With over 100 diseases that can attack ... top of the most wanted list? University of Illinois ... that more research is needed on the fungus that ... with cool, damp growing conditions but Macrophomina phaseolina ... and dry drought conditions. , "As the climate continues ...
(Date:7/9/2014)... a sparrow-sized fossil from China challenges the commonly held ... gained the ability to fly. The birdlike fossil is ... rather the remains of a tiny tree-climbing animal that ... Dinosaur Museum in Blanding, Utah, and Alan Feduccia of ... Springer,s Journal of Ornithology . , The fossil ...
Breaking Biology News(10 mins):Controlling contamination in open algae ponds for biofuels 2Climate change provides good growing conditions for charcoal rot in soybeans 2Climate change provides good growing conditions for charcoal rot in soybeans 3Researchers declassify dinosaurs as being the great-great-grandparents of birds 2
... An international research team led by Nanjing Agricultural ... of pear by an approach using the combination of ... only provides an invaluable new resource for breeding improvement ... on the genome evolution and other genome-wide comparative studies. ...
... 19, 2012 Medbox, Inc. (Ticker: MDBX) ( www.medboxinc.com ... they have received many inquiries from shareholders purchasing shares ... week,s volatile trading. These inquiries were responded to and ... rapport. In other news, the company ...
... person imagines moving a limb, cells in the part ... if trying to make the immobile limb work again. ... pathway between brain and muscle, the region where the ... recent years, neuroscientists and neuroengineers working in prosthetics have ...
Cached Biology News:Pear genome provides new insight into breeding improvement and evolutionary trace analysis 2Medbox Communicates with Shareholders, Comments on Pending Transition to OTC Bulletin Board, and Announces Appearance on Fox Business News Channel 2A better thought-controlled computer cursor 2A better thought-controlled computer cursor 3A better thought-controlled computer cursor 4A better thought-controlled computer cursor 5
(Date:1/15/2014)... This webinar will focus on EMA ... assessment in biosimilars. , Regulatory frameworks are evolving many ... however the complex nature of biopharmaceuticals makes the demonstration ... extremely challenging. Based on the specific aspects of biosimilar ...
(Date:1/15/2014)... January 15, 2014 DTS Language Services, ... Online Web Portal for Life Science organizations who need document ... the subject matter of their documents in advance with a ... reduce time-to-delivery of translations, often a critical factor in clinical ...
(Date:1/14/2014)... 2014 Date: Friday, April 11, 2014 , ... Club, 1360 Almshouse Road, Warrington, Pa. , Details: ... dedicated to finding a cure for hepatitis B and improving ... its annual Crystal Ball on Friday, April 11 at Warrington ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... of Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging ... to serve the wound care market. , Free report ... was restructured with a seasoned management team and Board, ...
Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... LA JOLLA, Calif., Feb. 23, 2011 ... to providing personalized treatment information to women with ... been appointed Executive Director, a new position for ... to lead the foundation as it increases its ...
... CITY, Calif., Feb. 23, 2011 Ingenuity® Systems, ... for life science researchers, today announced a research ... for next-generation sequencing (NGS) data analysis. Covance Genomics ... the drug discovery and development industry, and Ingenuity ...
... in German . , Further development of ... at reasonable costs. In the past, integration density of ... of this strategy, future components will have to reach ... Center for Functional Nanostructures (CFN) and IPCMS have now ...
Cached Biology Technology:The Clearity Foundation Hires Hillary Theakston as Executive Director 2The Clearity Foundation Hires Hillary Theakston as Executive Director 3Ingenuity Systems and Covance Collaborate to Support Next-Generation Sequencing Research 2Smallest magnetic field sensor in the world 2
... of SYBR Green I or FAM in ... fluorophores in the second channel including ... a multitude of applications such as RT-qPCR ... offers precision thermal control and a temperature ...
... identification and library manager ... that adds capabilities for ... identification of unknowns against ... of the same taxon ...
Fingerprinting II comparative quantification and polymorphism analysis is an optional module that adds comparative quantification and polymorphism analysis functions to the Fingerprinting II Basic so...
... or for use in an automated line. ... disposable manifold provides greater ease, speed, convenience, ... StatMatic II can be connected to a ... fill a microplate with one-handed operation -- ...
Biology Products: