Navigation Links
Secrets of a precision protein machine
Date:4/15/2011

"pseudo-DNA" to whose backbone the polymerase can start adding DNA nucleotides to match with the template. The polymerase keeps on adding nucleotides until it runs into the RNA primer from the previous start.

This doesn't happen often on the so-called leading strand of the unwound DNA, where the polymerase usually proceeds smoothly, as if on a one-way street. The one-way direction, named for the chemical composition of the nucleotides at opposite ends of a DNA strand, is 5' to 3' (five-prime to three-prime).

On the lagging strand, however, the one-way street runs in the opposite direction, so replication has to be done in many little discrete fragments assembled "backwards," each started by an RNA primer. Called Okazaki fragments, these are only about 100 nucleotides long in humans, and some 50 million of them are added to the lagging strand during a human cell's replication.

When polymerase runs into the RNA primer of a previous fragment it peels it away like a chisel, leaving a long tail or "flap" of excess single-strand DNA at the 5' end. This flap must be clipped off before the new fragment can be joined failure to do this accurately would leave a gap or overlap that could cause a genetic mutation or rearrangement resulting in damaged chromosomes. FEN1 cuts off the flap and precisely prepares it for joining to the newer fragment, which is also left with a tiny flap, known as the 3' overhang.

Just how does FEN1 do the job? Tainer notes that many important structural features of human FEN1 were known from studies in 2007 by Shigeru Sakurai of Japan's Nara Institute of Science and Technology and from work on related FEN from microorganisms known as archaea. These studies were done without FEN1 acting on DNA, however, and many questions remained.

For example, FEN1 was long thought to move into position by sliding down the 5' flap to the incision site, like a bead on a string. The 5' flap passes through an opening
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

0

GOOD

Page: 1 2 3 4 5

Related biology news :

1. Breaking the mucus barrier unveils cancer cell secrets
2. Uncovering the genome secrets of the Blackleg fungus
3. A pesky bacterial slime reveals its survival secrets
4. Unlocking the secrets of a plants light sensitivity
5. Unlocking the secrets of a plant’s light sensitivity
6. Discovery of the secrets that enable plants near Chernobyl to shrug off radiation
7. Air flows in mechanical device reveal secrets of speech pathology
8. Pigs reveal secrets: New research shines light on Quebec industry
9. Looking for secrets to drug addiction in our blood
10. Neutrons helping ORNL researchers unlock secrets to cheaper ethanol
11. Ancient brew masters tapped antibiotic secrets
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Secrets of a precision protein machine
(Date:5/21/2013)... Using a "patient monitoring" device attached to a whale ... time how fishing lines changed a whale,s diving and ... whales, ability to eat and migrate, depletes their energy ... can result in a slow death. , The scientists ... a Dtag to a two-year-old female North Atlantic right ...
(Date:5/21/2013)... researchers have developed a new method for delivering ... in the cell surface. The technique could find ... biological fields. , Bulk electroporation a technique ... nanopores in the cell membrane that are caused ... an increasingly popular method of cell transfection. (Cell ...
(Date:5/21/2013)... battle? Try staying fit in space, where living quarters ... , That,s the challenge a group of Michigan State ... million grant from NASA. Their goal is to keep ... "You could give astronauts the best exercise regimen there ... to it, it isn,t going to help," said project ...
Breaking Biology News(10 mins):Study reveals how fishing gear can cause slow death of whales 2Study reveals how fishing gear can cause slow death of whales 3Single-cell transfection tool enables added control for biological studies 2Fueling fitness on the final frontier 2
... Park was created to provide a haven for both ... University of Gothenburg have analysed species living on the ... determine which marine areas require special protection., , "It,s ... the national park. That,s why I,ve done what experienced ...
... Institute for Biologically Inspired Engineering at Harvard University ... exceptional strength, toughness, and versatility of one of ... and biocompatible, the new material, called "Shrilk," could ... be used safely in a variety of medical ...
... , LJUBLJANA, SLOVENIARidge and furrow rainwater-harvesting (RFRH) systems with ... conditions of northwest China to improve water availability and ... serve as rainwater harvesting zones, and bare or mulched ... increasingly recognized and are being promoted in many parts ...
Cached Biology News:Life on Kosterhavet's seabed analyzed 2Inspired by insect cuticle, Wyss Institute develops material that's tough and strong 2Improved rainwater harvesting system promising 2
(Date:5/22/2013)... , May 22, 2013  Superior Controls, ... Year 2013" by Business NH Magazine.  For the ... State organizations that distinguish themselves with extraordinary business ... proud to receive this honor," said ...  "As with any recognition, this award is a ...
(Date:5/22/2013)... New York, NY (PRWEB) May 22, 2013 ... My Cleaning Products said that there is a way to ... victims, it included on its latest post the details of ... spending too much money. , Mold could be prevented, My ... preventive measures, the company stated that a mold remediation must ...
(Date:5/22/2013)... The ability to sequence genomes ... they encode. There are now a suite of ... ribosome protected fragments) that dramatically expand our ability ... include Dr. Jonathan Weissman, an Investigator with the ... Medical Institute, who will present recent applications of ...
(Date:5/21/2013)... 2013 Custom Learning Designs, Inc.(CLD) ... solution using Tin Can API combined with its ... is a leading developer of training content and ... worldwide. , Tin Can API is a ... and trainers more accurately measure the Return on ...
Breaking Biology Technology:Superior Controls of Seabrook, NH named Business of the Year for 2013 by Business NH Magazine 2How to Kill Mold While Saving Thousands of Dollars on Mold Remediation Projects Explained by My Cleaning Products 2Monitoring Protein Synthesis One Codon at a Time Through Ribosome Profiling, a Webinar Hosted by Xtalks 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 3
... Aug. 17 Ingenuity® Systems, the leading provider ... announced the release of the white paper, "Best ... Process: Toxicogenomics."  The white paper is based on ... toxicology teams in drug discovery can leverage toxicogenomics ...
... Scientists and Engineers at The University of Nottingham have built ... ultrasound. These revolutionary transducers which are orders of ... up to 500 of the smallest ones could be placed ... at an early stage these devices offer a myriad of ...
... Results. -- Second quarter 2010 revenue rose 43% year-over-year to $47.5 million,-- Second ... diluted share." /> China BCT Reports Strong Second Quarter 2010 Results ... ... ...
Cached Biology Technology:Ingenuity Systems Announces Toxicogenomics Best Practices White Paper 2High definition diagnostic ultrasonics on the nanoscale 2China BCT Reports Strong Second Quarter 2010 Results 2China BCT Reports Strong Second Quarter 2010 Results 3China BCT Reports Strong Second Quarter 2010 Results 4China BCT Reports Strong Second Quarter 2010 Results 5China BCT Reports Strong Second Quarter 2010 Results 6China BCT Reports Strong Second Quarter 2010 Results 7China BCT Reports Strong Second Quarter 2010 Results 8China BCT Reports Strong Second Quarter 2010 Results 9China BCT Reports Strong Second Quarter 2010 Results 10China BCT Reports Strong Second Quarter 2010 Results 11China BCT Reports Strong Second Quarter 2010 Results 12China BCT Reports Strong Second Quarter 2010 Results 13China BCT Reports Strong Second Quarter 2010 Results 14China BCT Reports Strong Second Quarter 2010 Results 15
... peptide whose sequences are derived from mouse resistin protein. The ... S - M - P - L - C - P ... D - K - K - I - K - Q ... for neutralization and control experiments with the polyclonal antibody that reacts ...
... 2D Proteomic Imaging System, Basic Package ... Wide Dynamic Range: The ProXPRESS 2D Proteomic ... imaging system for both proteomic and general ... unparalleled flexibility, enabling the use of all ...
... microarrays designed for quantitative assays using ... built on the flexible and powerful ... customer specified sequences can be synthesized ... assistance with custom sequence design. These ...
... custom designed to meet your needs. Two ... features per array (2 arrays/1" x 3" ... (1 array/slide). Features ... This extended length delivers higher sensitivity compared ...
Biology Products: