Navigation Links
Scripps scientists develop first examples of RNA that replicates itself indefinitely
Date:1/9/2009

nt, perpetual self-replication.

Lincoln synthesized in the laboratory a large population of variants of the RNA enzyme that would be challenged to do the job, and carried out a test-tube evolution procedure to obtain those variants that were most adept at joining together pieces of RNA.

Ultimately, this process enabled the team to isolate an evolved version of the original enzyme that is a very efficient replicator, something that many research groups, including Joyce's, had struggled for years to obtain. The improved enzyme fulfilled the primary goal of being able to undergo perpetual replication. "It kind of blew me away," says Lincoln.

Immortalizing Molecular Information

The replicating system actually involves two enzymes, each composed of two subunits and each functioning as a catalyst that assembles the other. The replication process is cyclic, in that the first enzyme binds the two subunits that comprise the second enzyme and joins them to make a new copy of the second enzyme; while the second enzyme similarly binds and joins the two subunits that comprise the first enzyme. In this way the two enzymes assemble each other what is termed cross-replication. To make the process proceed indefinitely requires only a small starting amount of the two enzymes and a steady supply of the subunits.

"This is the only case outside biology where molecular information has been immortalized," says Joyce.

Not content to stop there, the researchers generated a variety of enzyme pairs with similar capabilities. They mixed 12 different cross-replicating pairs, together with all of their constituent subunits, and allowed them to compete in a molecular test of survival of the fittest. Most of the time the replicating enzymes would breed true, but on occasion an enzyme would make a mistake by binding one of the subunits from one of the other replicating enzymes. When such "mutations" occurred, the resulting recombinant enzymes a
'/>"/>

Contact: Keith McKeown
kmckeown@scripps.edu
858-784-8134
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Scripps Research scientists shed light on how DNA is unwound so that its code can be read
2. Dolphin population stunted by fishing activities, Scripps/NOAA study finds
3. Scripps research team defines new painkilling chemical pathway
4. Scripps research scientists identify blood component that turns bacteria virulent
5. Scripps research scientists identify compounds for stem-cell production from adult cells
6. Scripps Florida scientists awarded $1.5M to fight major water and food parasites
7. Scripps research team sheds light on immune system suppression
8. Scripps research team solves structure of beneficial virus
9. Oceans on the precipice: Scripps scientist warns of mass extinctions and rise of slime
10. Scripps scientists will assess Beijing Olympics air pollution control efforts
11. Scripps study sets high economic value on threatened Mexican mangroves
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/15/2014)... 2020 how high sea levels will rise into the ... Florida International University scientist Rene Price. , Price is ... Coastal Everglades Long-Term Ecological Research (LTER) site, one of ... reefs to deserts, mountains to salt marshes around the ... one of the most certain consequences of climate change. ...
(Date:4/15/2014)... If an insect drew a line as it ... a tangled mess. But there,s method to that mess, ... engineering and physics, who tries to find simple physical ... https://cornell.box.com/tbeetle , It turns out the tiger beetle, ... reorientation dance as it chases its prey at blinding ...
(Date:4/15/2014)... 15, 2014) The American Association of Anatomists (AAA) ... All awards will be presented during the Closing Awards ... on Tuesday, April 29th at 7:30 p.m. during AAA,s ... The 2014 award winners are: , ... of the Icahn School of Medicine at Mount Sinai, ...
Breaking Biology News(10 mins):Long-term predictions for Miami sea level rise could be available relatively soon 2Photo: Tiger beetle's chase highlights mechanical law 2American Association of Anatomists 2014 award winners 2
... 2008. A research project supported by the BBVA Foundation ... of Applied Bioacoustics at the UPC (Universitat Politcnica de ... measuring cetacean hearing sensitivity. , This audiogram measurement ... allowing assessments to be run on the survival chances ...
... December 15 WCC Services, the,leading provider of ... 6 of its flagship product, ELISE, is now ... hundreds of applicants for,each job opening making it ... job.,Security concerns are also at peak levels, requiring ...
... The American Society for Microbiology (ASM) has been honored ... two of its public outreach initiatives. The awards ... The Society,s MicrobeWorld Video series received the award ... online video series strives to increase public understanding and ...
Cached Biology News:A BBVA Foundation project comes up with a system enabling detection of cetacean hearing loss 2A BBVA Foundation project comes up with a system enabling detection of cetacean hearing loss 3WCC Announces Version 6 of its ELISE Smart Search and Match Platform for Employment and Biometric Matching 2
(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA ... disease. One of these latent viruses is the Epstein ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... by the theory, a study found that RA patients ... (1). , What is Microcompetition? , Dr. Hanan ...
(Date:1/15/2014)... Sunnyvale, CA (PRWEB) January 15, 2014 ... of high throughput research solutions, today announced that ... companies, has received delivery of Freeslate’s CM ... form screening. , Lupin, headquartered in Mumbai, ... quality, affordable generic and branded formulations and Active ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, ... p.m. , Location: Warrington Country Club, 1360 Almshouse Road, ... Foundation, the only national nonprofit organization solely dedicated to ... quality of life for those affected worldwide, will host ... Warrington Country Club in Warrington, Pa. , Each ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider ... major scientific, regulatory, business, and media events in ... , today announced its former associate Virginia Cox , ... office. Virginia Cox re-joins ... Commissioner for the Office of External Affairs at the U.S. ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2
... Oct. 26, 2011 The following is a letter ... Ltd. (OTCBB: HADSY), a publicly traded portfolio of biotech ... Hadassah University Hospital. Dear Fellow Investors, ... establishment by the Hadassah University Hospital and its IPO ...
... this smorgasbord of recent salmonella outbreaks in the United ... 2011 alone, and the list goes on, according to ... perhaps not for long, thanks to a promising new ... bacteria before contaminated food or animals reach the marketplace. ...
... and IPSWICH, Mass., Oct. 25, 2011 SwitchGear ... services for studying regulatory elements in the human genome, ... Inc. (NEB) to create a new dual reporter assay ... Luciferase Assay System and NEB,s BioLux® Cypridina ...
Cached Biology Technology:Summary Letter to Hadasit Bio-Holdings Ltd. Shareholders 2Summary Letter to Hadasit Bio-Holdings Ltd. Shareholders 3Summary Letter to Hadasit Bio-Holdings Ltd. Shareholders 4Summary Letter to Hadasit Bio-Holdings Ltd. Shareholders 5Summary Letter to Hadasit Bio-Holdings Ltd. Shareholders 6SwitchGear Genomics and New England Biolabs Develop New Multiplex Reporter Assay System 2
Applications: Beadlyte® assay Stability: 2 years at 4°C (lyophilized) or 6 months at -20°C (rehydrated) ...
... Mouse monoclonal [SPM278] to 67kD Laminin ... lung carcinoma N592 live cells (Human). ... Not yet tested in other species. ... major glycoproteins of basement membrane, displays multiple ...
Hybridization Buffer 3.6 ml...
...
Biology Products: