Navigation Links
Scripps research team reveals how an old drug could have a new use for treating river blindness
Date:2/9/2010

d drugs and 432 are foreign-approved drugsto screen for active compounds.

"We were looking for a molecule that had a dramatic effect on chitanase specific to O. volvulus," Gloeckner explains. "The chitinase's enzymatic activity was monitored by a fluorescent signal. A library member was scored as a "hit" when a decrease in the signal was observed. Simply stated when a huge decrease in the signal was observed, the enzyme was essentially "knocked-out."

The screening efforts identified four known drugs namely levfloxacin, lomefloxacin, dexketoprofen, and closantel. Of these, only closantel was found to exhibit potent enough inhibition to warrant further investigation.

Cross-Country Collaboration

The next step was to find out if closantel would work in vivo, in the larvae of O. volvulus.

"The molting process is considered a potential new target for chemotherapy against onchocerciasis," Gloeckner explains. "And since chitinases may play a key role in molting, we wanted to determine the effect of closantel on this process." Specifically, the researchers were interested in how clostanel would disrupt molting from the L3 to L4 stage of the larvae, a critical step that occurs within the human host.

That's when the Scripps Research team enlisted the help of Sara Lustigman's laboratory at the Lindsley F. Kimball Research Institute at the New York Blood Center. Lustigman's team cultured L3-stage larvae in the presence of increasing concentrations of closantel, and the number of larvae was determined on day six. The results? Closantel completely prevented molting from the L3 to L4 stage.

Gloeckner was excited by this finding. "Based on its specificity, potency, and ease of synthesis, closantel or one its analogues might represent a promising alternative or adjunct therapy in combination with ivermectin for the treatment of onchocerciasis," he says.

Gloeckner adds that, bas
'/>"/>

Contact: Keith McKeown
kmckeown@scripps.edu
858-784-8134
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Scripps Research scientists find two compounds that lay the foundation for a new class of AIDS drug
2. Scripps Research scientists create new way to screen libraries of 10 million or more compounds
3. Scripps Research team wins global race to achieve landmark synthesis of perplexing natural product
4. Scripps Florida scientists show lifeless prions capable of evolutionary change and adaptation
5. Scripps research team develops technique to determine ethnic origin of stem cell lines
6. Scripps Research scientists crack mystery of proteins dual function
7. Scripps Research team uncovers chemical basis for extra quality control in protein production
8. Scripps research scientists find new link between insulin and core body temperature
9. Scripps-led study shows ocean health plays vital role in coral reef recovery
10. Scripps Research scientists observe human neurodegenerative disorder in fruit flies
11. Scripps studies offer new picture of Lake Tahoes earthquake potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/21/2014)... A team of University of California, Riverside Bourns College ... under the grates of a backyard grill reduces by 70 ... , The one-eighth inch thick steel carbon plate with an ... cutting a slit into the grill to allow the plate ... adopted by grill manufacturers who could sell new grills with ...
(Date:7/21/2014)... team at the INRS nergie Matriaux Tlcommunications Research ... spatial and temporal resolution greatly exceeding that obtained ... femtosecond laser source to shoot a molecular movie ... An article presenting the advancement was recently published ... , "The approach we developed combines multiphoton ...
(Date:7/21/2014)... change, due to greenhouse gasses emitted by livestock. New ... and that beef cattle are responsible for far more ... is published by Climactic Change . , ... to climate change. It is released by vehicles, industry, ... greenhouse gas totals. But methane and nitrous oxide are ...
Breaking Biology News(10 mins):Catching grease to cut grill pollution 2Climate: Meat turns up the heat 2
... Information on proteins is critical for understanding how cells ... are easy to extract and study, it is far ... are responsible for exchanging elements essential to our health, ... Now Prof. Nir Ben-Tal and his graduate students ...
... strategies used to assess the environmental risks posed by ... reveals a researcher from the University of Gothenburg (Sweden) ... "Above all, we need to include the combined effects ... as it really is," says Sara Brosch from the ...
... 2010 American Academy of Ophthalmology (AAO) - Middle ... a report on beta carotene,s ability to ... diseases. The AAO-MEACO meetingthe world,s largest, most comprehensive ... 19 at McCormick Place, Chicago. 9-cis Beta-Carotene ...
Cached Biology News:Breakthrough: With a chaperone, copper breaks through 2Environmental risk assessment of pharmaceuticals inadequate 2
(Date:7/22/2014)... Dr. Williams says, “Bush has joined the ... the greatest health care system in the world is ... while trying to serve the public. Unfortunately, Bush has ... an enormous system that none of them can first ... series of Open Letters ( http://is.gd/wdU2CM ) provides a ...
(Date:7/21/2014)... Syracuse University,s College of Arts and Sciences has ... of stainless nanoparticles. , Mathew M. Maye, associate ... $360,000 grant from the National Science Foundation (NSF). ... stainless alloy nanostructures, the results of which may ... batteries. , Maye,s approach is novel, ...
(Date:7/21/2014)... Sunset, LA (PRWEB) July 21, 2014 ... poor sleep quality are closely correlated with depression. ... Institutes of Health: "About three quarters of ... present in about 40% of young depressed adults ... in females. The symptoms cause huge distress, have ...
(Date:7/18/2014)... , July 18, 2014 Regeneron Pharmaceuticals, Inc. ... quarter 2014 financial and operating results on Tuesday, August ... Company will host a conference call and simultaneous webcast ... Conference Call InformationTo access this call, dial (888) ... the webcast may be accessed from the ,Events and ...
Breaking Biology Technology:Dr. Ira Williams Asks What’s Wrong With Jonathan Bush’s Book, Where Does It Hurt? 2Syracuse University chemist to use NSF grant to study materials chemistry, nanoscience 2Open Label Data on Enhanced Sleep Released by JayMac Pharmaceuticals, LLC 2Open Label Data on Enhanced Sleep Released by JayMac Pharmaceuticals, LLC 3
... N.J., Oct. 8 Managed Health Care,Associates, Inc. ... that it plans to open the 2008 MHA ... Secretary, Health and Human Services,(2001-2005), former four-term Governor ... Independent Chairman, Deloitte,Center for Health Solutions. Marking the ...
... announced today that it has entered into a ... producer of infant,formula products including the Similac(R) Advance(R) ... and ARA supplier for all of its,infant formula ... with,Abbott having the right to terminate the arrangement ...
... patients to benefit from rapid, long-lasting symptom control with ... ... fumarate -, NAPA, Calif., Oct. 8 Dey, L.P., ... the launch of Perforomist(TM),(formoterol fumarate) Inhalation Solution, which will be ...
Cached Biology Technology:MHA Announces: Tommy Thompson to be Keynote Speaker at The MHA 5th Annual Business Summit 2Martek Signs Multi-Year Worldwide Sole Source Supply Agreement with Abbott 2Mylan Inc. Subsidiary, Dey, L.P., Announces that Perforomist(TM) (Formoterol Fumarate) Inhalation Solution is Now Available for Maintenance Treatment of COPD 2Mylan Inc. Subsidiary, Dey, L.P., Announces that Perforomist(TM) (Formoterol Fumarate) Inhalation Solution is Now Available for Maintenance Treatment of COPD 3Mylan Inc. Subsidiary, Dey, L.P., Announces that Perforomist(TM) (Formoterol Fumarate) Inhalation Solution is Now Available for Maintenance Treatment of COPD 4Mylan Inc. Subsidiary, Dey, L.P., Announces that Perforomist(TM) (Formoterol Fumarate) Inhalation Solution is Now Available for Maintenance Treatment of COPD 5Mylan Inc. Subsidiary, Dey, L.P., Announces that Perforomist(TM) (Formoterol Fumarate) Inhalation Solution is Now Available for Maintenance Treatment of COPD 6
Phospho-Cyclin D1 (Thr286) Antibody...
STX18 Immunogen: STX18 (NP_058626, 101 a.a. ~ 200 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline ( Abpromise for all tested applications)....
Goat polyclonal to Factor VIII, prediluted ( Abpromise for all tested applications). Antigen: Full length native protein (purified) (Human): Factor VIII from blood. Entrez GeneID: 2157 Sw...
Biology Products: