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Scripps oceanography researchers engineer breakthrough for biofuel production
Date:11/21/2013

Researchers at Scripps Institution of Oceanography at UC San Diego have developed a method for greatly enhancing biofuel production in tiny marine algae.

As reported in this week's online edition of the Proceedings of the National Academy of Sciences, Scripps graduate student Emily Trentacoste led the development of a method to genetically engineer a key growth component in biofuel production.

In the quest to loosen humanity's dependence on traditional fossil fuel consumption, and with it rising concentrations of carbon dioxide and their damaging impacts on the environment, finding economically viable fuels from biological sources has been elusive.

A significant roadblock in algal biofuel research surrounds the production of lipid oils, the fat molecules that store energy that can be produced for fuel. A catch-22 has stymied economically efficient biofuel production because algae mainly produce the desired lipid oils when they are starved for nutrients. Yet if they are limited in nutrients, they don't grow well. With a robust diet algae grow well, but they produce carbohydrates instead of the desired lipids for fuel.

In a significant leap forward that clears the lipid production hurdle, Trentacoste and her colleagues used a data set of genetic expression (called "transcriptomics" in laboratories) to target a specific enzyme inside a group of microscopic algae known as diatoms (Thalassiosira pseudonana). By metabolically engineering a "knock-down" of fat-reducing enzymes called lipases, the researchers were able to increase lipids without compromising growth. The genetically altered strains they developed, the researchers say, could be produced broadly in other species.

"These results demonstrate that targeted metabolic manipulations can be used to increase accumulation of fuel-relevant molecules. with no negative effects on growth," said Trentacoste. "We have shown that engineering this pathway is a uni
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Contact: Mario Aguilera
scrippsnews@ucsd.edu
858-534-3624
University of California - San Diego
Source:Eurekalert

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