Navigation Links
Scripps Research team uncovers chemical basis for extra 'quality control' in protein production
Date:12/9/2009

e results showed that one reason for the difficulty AlaRS has in distinguishing alanine from serine and glycine is that the active site on the AlaRS molecule is a large, flexible pocket. Instead of acting as a rigid lock for a single key, the cavity flexes to hold not only its target alanine, but also similar-size molecules serine and glycine.

But serine and glycine are not exactly the same size as alanine. That's where the "serine paradox" comes in. Glycine is smaller and serine is larger than alanine. Current theory would not predict that molecules that are both larger and smaller than alanine would be a problem for AlaRS.

"The reason we call it a paradox is because none of the other tRNA synthesases have a problem mis-activating both a smaller and a bigger amino acid," said Guo. "Theoretically, the tRNA synthesases should have the most problem recognizing a smaller amino acid, because a smaller one can also go into the binding pocket. The smaller one is easy to understand. Now we explain the bigger one."

Unexpectedly, the new study's results also revealed that within AlaRS's binding pocket the acidic group of Asp235 creates an extra hydrogen bond with the larger serine molecule. This additional bond turns out to be the major force that helps to secure the misplaced serine in the pocket, despite its larger size. However, x-ray analysis showed that Asp235 is also critical for holding the amino group of alanine. Attempts by the Schimmel-Yang lab (and also most likely by eons of evolution) to replace Asp235 with another residue failed. In fact, the scientists found that to make a change that would eliminate the interaction with serine would also impact negatively on the interaction with the correct amino acid, alanine. So, nature developed another solution by creating AlaXp, which is specifically designed to provide a second check and eliminate any serine that is attached to tRNA (Ala).

Together, AlaRS's large, flexible pocket an
'/>"/>

Contact: Keith McKeown
kmckeown@scripps.edu
858-784-8134
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Scripps research scientists find new link between insulin and core body temperature
2. Scripps-led study shows ocean health plays vital role in coral reef recovery
3. Scripps Research scientists observe human neurodegenerative disorder in fruit flies
4. Scripps studies offer new picture of Lake Tahoes earthquake potential
5. Scripps scientists help decode mysterious green glow of the sea
6. Scripps scientists find structure of a protein that makes cancer cells resistant to chemotherapy
7. Scripps research scientists watch as individual alpha-synuclein proteins change shape
8. Scripps research team identifies key molecules that inhibit viral production
9. Historical photographs expose decline in Floridas reef fish, new Scripps study finds
10. Scripps scientists create first crystal structure of an intermediate particle in virus assembly
11. Scripps scientists develop first examples of RNA that replicates itself indefinitely
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2015)... 13, 2015 According to ... Biometrics Market Forecast & Opportunities, 2020", the global biometrics ... around 14% till 2020. The biometrics market is ... implementation and review of biometric management systems. The ... products with greater efficiency, are resulting in increasing ...
(Date:4/10/2015)... -- Research and Markets ( http://www.researchandmarkets.com/research/cjk4gb/security ) ... Profiles - NEC" report to their offering. ... continue to supply a range of IT security management ... focus on the development of a Big Data and ... the Asia-Pacific region is a ...
(Date:4/8/2015)... CHARLOTTE, N.C. , April 8, 2015 /PRNewswire/ ... technology solutions and services, and Morpho, a leading ... in partnership to produce the market,s most advanced ... Infinisource,s iSolved NXG time clock is setting ... attendance and labor data for the small and ...
Breaking Biology News(10 mins):Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2NEC Security Competitive Profile 2015 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3
... PORTLAND, Ore. June 10, 2013. A recent government report reveals ... western Washington has doubled over the past 30 years, and ... , "Resource lands in Washington are being lost at a ... Gray, a research ecologist at the Forest Service,s Pacific Northwest ...
... , June 11, 2013 Intelligent building industry ... costing (LCC) as a valid prerequisite in the process ... presently, use of LCC to determine long-term return on ... are a long-term commitment in most cases, making well-informed ...
... and devastating disorder characterized by the breakdown of cognition ... affecting people throughout history. Scientists believe that several genes ... that no single gene causes the disease by itself. ... environment are necessary for schizophrenia to develop and that ...
Cached Biology News:Land use changes, housing demographics shift in Washington State 2A Future-Proof Approach to Intelligent Buildings 2A Future-Proof Approach to Intelligent Buildings 3A Future-Proof Approach to Intelligent Buildings 4
(Date:4/24/2015)... 24, 2015  Navitas Life Sciences is delighted to announce ... Clinical and Regulatory Services. Shalabh is a proven leader in ... CEO of Kinapse, as well as a Strategy consultant at ... - http://photos.prnewswire.com/prnh/20150423/201261LOGO ... "I am delighted to welcome Shalabh into this critical role ...
(Date:4/23/2015)... 2015 Ralco announced today that it ... (ABN) for the exclusive worldwide rights to sell and ... is a new in-line milk system that increases the ... to move supplementation milk cups within a farrowing facility ... technology lets producers get Birthrightâ„¢ milk to the right ...
(Date:4/23/2015)... 23, 2015 /CNW/ - A new and innovative service ... North America,s largest packaging ... Silver in the "Sustainability, service" category of the ... "Competitor Comparison" is a service that scores clients, ... Global peers. This benchmarking process shows clients how ...
(Date:4/23/2015)... , April 23, 2015  Neurocrine Biosciences, Inc. ... will report its first quarter 2015 results before the ... will then host a live conference call and webcast to ... Thursday morning, April 30, 2015 at 8:00 a.m. Eastern ... access the live conference call by dialing 866-952-1906 (US) ...
Breaking Biology Technology:Shalabh Kumar Joins Navitas Life Sciences to Lead their Clinical and Regulatory Services Teams 2Ralco Acquires License to Birthrightâ„¢ Moveable Milk Cup 2Neurocrine Biosciences Announces Conference Call and Webcast to Report First Quarter 2015 Results 2
... biotech industry, a well educated work force and citizens who ... billion budget deficit and a per capita income almost $1000 ... average in citizens with a 4-year degree, which means we ... state of our economy presents a complex problem that cannot ...
... Companies from nine different states staked their territory Tuesday ... presentations in hopes of catching the eye of venture ... Venture Conference at Monona Terrace, Madison. , ,According to ... representing not only life sciences but the energy, medical ...
... genetic testing know well the importance of federal ... ingredients of many genetic tests. Recent efforts ... opportunities for Wisconsin companies. , ,Under current regulations, ... drug companies, are permitted to sell many ASRs ...
Cached Biology Technology:WARF Collaboration a Win-Win for Wisconsin 2WARF Collaboration a Win-Win for Wisconsin 3Investors Pick Favorites at Life Science Conference 2Investors Pick Favorites at Life Science Conference 3Support Regulatory Change to Boost Wisconsins Biotech Sector 2Support Regulatory Change to Boost Wisconsins Biotech Sector 3
The filter 530DF28 for the Molecular Imager FX is a band pass filter for ECL Plus, AttoPhos, SYBR Green, Alexa Fluor 488, FITC, Cy2, and Pro-Q Emerald dyes....
... gel cell is a stand-alone apparatus that ... applications. The upper buffer chamber can accommodate ... long for isoelectric focusing (IEF) applications. The ... cooling core, lower buffer chamber, lid with ...
... Direct In Situ Apoptosis Detection ... situ by the direct TUNEL ... tagged with a directly labeled ... fluorescence staining for 40 samples ...
... is a highly advanced digital imaging ... imaging and automation technology that is ... archving/retrieval and colored professional reporting. The ... fastest scanning and detection modules for ...
Biology Products: