Navigation Links
Scripps Research team overcomes major obstacle for stem cell therapies and research
Date:9/8/2011

biology Jeanne Loring, was looking for a new route to solve the purification and safety problems. The group recently began experimenting with chip-based tools known as lectin arrays. At various points on these devices, plant-produced proteins called lectins are attached. These lectins bind with specific sugars including some found on the surface of cells.

Working in the lab with cellular components, rather than whole cells, the Loring team first found that specific combinations of sugars and proteins known as glycoproteins on stem cells reliably bind to certain lectins. They were then able to exploit this connection to purify cell mixtures.

"When we discovered there was a specific binding pattern, we decided we should just go for it and see whether we could use the lectins to purify cells," said Yu-Chieh Wang, the first author of the research article. "We tested the idea and it works very well, and lectins are readily available and inexpensive."

After identifying the lectin that bound best with stem cells, the group took the work to the next level to show that they could actually separate out stem cells. To accomplish this, they first attached the lectin to tiny beads. Then they exposed these beads to mixtures of stem cells along with non-stem cells.

The researchers used a range of different types of both embryonic stem cells and induced pluripotent cells, which are embryonic stem cell-like cells that are produced by inserting certain genes into skin cells. They included cell lines from both Scripps Research and the labs of their collaborators in Japan and the United States.

In every case, the team found that the stem cells bound remarkably well to the beads, while the cells that washed past were almost all non-stem cells; this meant that both cell types could be collected separately for use in research or in treatments.

Purity's Potential

Possible uses for the new technique are essentially
'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Scripps Research scientists pinpoint shape-shifting mechanism critical to protein signaling
2. Scripps Research scientists establish new class of anti-diabetic compound
3. Scripps Research scientists produce first stem cells from endangered species
4. Scripps Research scientists help pinpoint cause of stress-related DNA damage
5. Scripps Research scientists expand knowledge of cell process involved in many diseases
6. Scripps Research scientists create vaccine against heroin high
7. Scripps Research scientists solve mystery of nerve disease genes
8. Scripps awarded $7.9 million NIH grant for research to find root cause of heart attack
9. Scripps study finds plastic in 9 percent of garbage patch fishes
10. Scripps Research Institute Scientist Knighted by Italian Republic
11. Scripps Research team sheds new light on how blood clots form
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scripps Research team overcomes major obstacle for stem cell therapies and research
(Date:7/24/2014)... new study has identified two factors that characterize ... the production of highly qualified physics teachers. Specifically, ... champion physics teacher education in combination with institutional ... remain viable. Science, Technology, Engineering and Math (STEM) ... the study points the way for institutions seeking ...
(Date:7/24/2014)... Some sticky research out of York University shows ... species of toxic grass fungus: moose saliva (yes moose ... Letters , "Ungulate saliva inhibits a grassendophyte mutualism" shows ... fescue grass (which hosts a fungus called epichlo festucae ... growth and less toxicity. , "Plants have evolved defense ...
(Date:7/24/2014)... at Albert Einstein College of Medicine of ... J. Fox Foundation for Parkinson,s Research to translate a ... This drug discovery project will test chemical compounds on ... a drug that acts on an underlying cause of ... help many people manage their symptoms, we are eager ...
Breaking Biology News(10 mins):Creating sustainable STEM teacher preparation programs 2Moose drool inhibits growth of toxic fungus: York U research 2Albert Einstein College of Medicine receives grant from the Michael J. Fox Foundation to fund drug discovery project targeting Parkinson's 2Albert Einstein College of Medicine receives grant from the Michael J. Fox Foundation to fund drug discovery project targeting Parkinson's 3
... are elementary building blocks of life. They often perform ... to fold into three-dimensional structures. Misfolding of proteins leads ... strategies did nature develop over the course of evolution ... chemist Dr. Frauke Grter (Heidelberg Institute for Theoretical Studies) ...
... January 31, 2013 -- Deborah E. Wiley, Chair of ... JWa & JWb), announced today that the twelfth annual ... Dr. Michael Young, Rockefeller University, Dr. Jeffrey Hall, Brandeis ... The Wiley Prize is being awarded to Dr. Young, ...
... N.C. In the last decade, a new strain of ... everyone at risk of contracting the dangerous bacterial infection. This ... a chunk of genes not shared by any other strains, ... the bacteria to survive and persist in the community. ...
Cached Biology News:Protein origami: Quick folders are the best 2Protein origami: Quick folders are the best 312th annual Wiley Prize in Biomedical Sciences awarded 212th annual Wiley Prize in Biomedical Sciences awarded 3UNC study may lead to treatments that are effective against all MRSA strains 2
(Date:7/24/2014)... , July 24, 2014 Research and ... "Global Cell Culture Market 2014-2018" report to their ... Cell culture is the in vitro growth of ... research, cell studies, and biopharmaceutical production. Cell culture is ... Cell culture instruments and cell culture consumables are necessary ...
(Date:7/24/2014)... PARK, Calif. , July 24, 2014 ... seven-year contract with the National Institute of Allergy and ... Health, to conduct preclinical development of potential therapies for ... of drugs to treat HIV and AIDS and the ... well as microbicides for preventing sexual transmission of HIV. ...
(Date:7/24/2014)... Dallas, Texas (PRWEB) July 24, 2014 ... China Potassium Sulphate Industry” is a professional and ... The report introduces Potassium Sulphate basic information, ... structure and industry overview. This research covers the ... well as global industry analysis covering macroeconomic environment ...
(Date:7/24/2014)... balances – 4 steps – One Recommendation: the web-based ... narrows down the mass of product choices to a ... , First, select the working environment, then enter the ... define the accuracy – it’s really that simple. Specifying ... to models that fit best with the customer’s industry ...
Breaking Biology Technology:Global Cell Culture Market 2014-2018: Key Vendors are Becton, Dickinson and Co, EMD, GE Healthcare, Lonza Group, Sigma Aldrich and Thermo Fisher Scientific 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 4
... Schering-Plough Assumes Exclusive Worldwide Marketing Rights for ... N.J., May 19 Schering-Plough Corporation (NYSE: ... to its global collaboration with Novartis for ... therapies for the treatment of asthma and ...
... May 18 Solvay Pharmaceuticals announces it ... a company specialized in enabling and accelerating global ... China. Through this agreement, Solvay Pharmaceuticals will gain ... specifically on compounds in the cardiovascular area. ...
... Entire ALS Community to Join a Nationwide Network of ... ALS Therapy Development Institute (ALS TDI) announced its ... designed to increase the awareness and understanding of ALS ... To accomplish its goal, the campaign brings together three ...
Cached Biology Technology:Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 2Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 3Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 4Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 5Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 6Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 7Schering-Plough Announces Changes to its Global Collaboration with Novartis to Develop Combination Therapies for Asthma and COPD 8Solvay Pharmaceuticals Announces Strategic Partnership With HUYA to Access new Drug Candidates From China 2Solvay Pharmaceuticals Announces Strategic Partnership With HUYA to Access new Drug Candidates From China 3Campaign to Increase the Awareness and Understanding of Lou Gehrig's Disease Reaches 22 U.S. States 2Campaign to Increase the Awareness and Understanding of Lou Gehrig's Disease Reaches 22 U.S. States 3
Anti-human VEGFR-1/Flt-1, clone Flt-1/EWI, Monoclonal Antibody...
100 ug affinity purified rabbit anti-JIP-1 polyclonal antibody. Reacts with mouse and rat. Perform well in Western blotting. Other applications have not been tested. Storage Temperature: -20C Shi...
...
... Neuron navigator 1 ( Abpromise for all ... peptide conjugated to KLH derived from within residues ... 1. (Note: the amino acid sequence is ... ab32208 .) Entrez Gene ID: ...
Biology Products: