Navigation Links
Scripps Research scientists expand knowledge of cell process involved in many diseases
Date:8/16/2011

JUPITER, FL, August 16, 2001 As part of a joint research effort with the University of Michigan, scientists from the Florida campus of The Scripps Research Institute have for the first time defined the structure of one of the cell's most basic engines, which is required for cell growth, as it assembles from its components.

The study reveals a series of redundant mechanisms that assure production of these critical structures while avoiding any missteps that could lead to their destruction or to the production of incorrect cellular building blocks. These findings throw new light on a process that is integrally involved in a number of disease states, including cancer and Alzheimer's disease.

The study, published on August 11, 2011, in the advance online edition of the prestigious journal Science, reveals the structure of an assembly intermediate of the small ribosomal subunit.

Ribosomes, which are large macromolecular machines required for cell growth in all organisms, catalyze the production of proteins in all cells. They read the genetic code carried by messenger RNA, and then catalyze or translate that code into proteins within cells, assembling them from amino acids.

Understanding the process of ribosome assemblywhich involves almost 200 essential proteins known as "assembly factors" in addition to the four RNA molecules and 78 ribosomal proteins that are part of the mature ribosomeis a potentially fruitful area of research because of the importance of ribosome assembly for cell growth. The link between defects in ribosome assembly and cancer clearly points to this pathway as a new target for anti-cancer drugs.

In the current study, the scientists used cryo-electron microscopy (where samples are studied at temperatures of � C) to image the 40S ribosome structure.

"This is the best-defined ribosomal assembly intermediate we have ever had with true structural information on the location of each assembly factor," said Katrin Karbstein, an associate professor at Scripps Florida and one of the senior authors of the study. "It will be helpful in determining what's going on in what is still a relatively unknown process."

While most ribosome assembly takes place in the nucleolus, a protein-nucleic acid structure inside the nucleus, the final maturation process occurs in the cytoplasm, the "general" cellular compartment where protein translation occurs. In the cytoplasm, these pre-mature ribosomal subunits encounter large pools of mature subunits, messenger RNA, and various translation factors.

This cellular stew presents a unique challenge, especially keeping the translation process from acting on the subunits prematurely, which would result in their rapid degradation or in the production of incorrectly assembled proteins, both processes with potentially lethal outcomes for the cell.

The new study shows that that the bound assembly factors cooperate with one another in a highly redundant and multi-pronged approach to prevent such occurrences, chaperoning the pre-40S subunits to keep them from falling victim to the translational apparatus.

"We had thought the role of assembly factors was to help mature this intermediate form of the ribosome," said Karbstein. "But our new research has shown that these assembly factors also prevent a number of unwanted things from happening. If one of these intermediate forms were to bind prematurely to a messenger RNA, there could be no protein produced, or worse, a wrong protein might be produced and that could lead to early cell death."

It's important to note that this is a single snapshot of the late-stage assembly process, Karbstein added. "We know better how the process works but this is by no means a final statement," she said.


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Related biology news :

1. Scripps Research scientists create vaccine against heroin high
2. Scripps Research scientists solve mystery of nerve disease genes
3. Scripps awarded $7.9 million NIH grant for research to find root cause of heart attack
4. Scripps study finds plastic in 9 percent of garbage patch fishes
5. Scripps Research Institute Scientist Knighted by Italian Republic
6. Scripps Research team sheds new light on how blood clots form
7. Scripps Research scientists awarded $2.2 million to develop treatment for multi-drug addiction
8. Scripps Research scientist wins $1.9 million grant to study malaria
9. Scripps Research scientists find way to block stress-related cell death
10. Scripps Research scientists find key mechanism in transition to alcohol dependence
11. Scripps Research scientists create new genetic model of premature aging diseases
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scripps Research scientists expand knowledge of cell process involved in many diseases
(Date:3/6/2017)... , March 6, 2017 ... sales technology, today announced Predictive Sales Coach TM ... infusing actionable sales intelligence into Salesforce. This unique ... enable their sales organizations with deep knowledge of ... allow for intelligent engagement. Predictive Sales Coach extends ...
(Date:3/2/2017)... -- Who risk to be deprived of its imprint ... https://www.reportbuyer.com/product/4313699/ WILL APPLE AND SAMSUNG CONFRONT ... sensors using capacitive technology represent a fast growing market, ... an increase of 360% of the number of fingerprint ... sensor market between 2014 and 2017 (source : N+1 ...
(Date:2/28/2017)... ANGELES , Feb. 28, 2017   Acuant ... verification software globally, announces significant enhancements to new and ... May 2016. New products include mobile and desktop Acuant ... DocX TM - a real time manual review ... core idScan® technology provides the fastest and most accurate ...
Breaking Biology News(10 mins):
(Date:3/24/2017)... YORK , March 24, 2017 ... ended the trading session at 5,817.69, down 0.07%; the ... at 20,656.58; and the S&P 500 closed at 2,345.96, ... as 4 sectors closed in green, 4 sectors finished ... day. This Friday, Stock-Callers.com has initiated reports coverage on ...
(Date:3/23/2017)... Research and Markets has announced the addition of the ... ... The Global Market for Bioproducts Should Reach $714.6 Billion by ... 8.9%, This research report quantifies the two ... segments: bio-derived chemicals, biofuels, pharmaceuticals (biodrugs and herbal/botanicals), biocomposite materials, ...
(Date:3/23/2017)... Branford, CT (PRWEB) , ... March 23, 2017 ... ... Counsel, LLC, was recently selected by the Connecticut Technology Council (CTC) as a ... honored at CTC’s thirteenth annual Women of Innovation Awards Dinner. , The dinner ...
(Date:3/23/2017)... YORK , March 23, 2017 ... look at four equities in the Biotech industry: Sangamo ... Synthetic Biologics Inc. (NYSE MKT: SYN), and Regulus Therapeutics ... 21 st , 2017, Credit Suisse upgraded its rating on ... by downloading their free report at: ...
Breaking Biology Technology: