Navigation Links
Scripps Research scientists create new way to screen libraries of 10 million or more compounds
Date:2/1/2010

day."

Ligands are compounds that attach to proteins and alter their expression, potentially affecting a particular biomolecular activity, say, a protein pathway involved in a disease.

The new method displays millions of compounds on the surface of resin-based beads, each type of compound on a different bead. The hits are culled from the beads using a unique magnetic signature and then transferred to a microarray formatglass slides or silicon chips that can hold large numbers of compounds on their surface. The microarray format allows quantitative comparison of binding affinity that can be carried out without the need for tedious re-synthesis of many different compounds.

In the study, the team used mixed peptide/peptoid librariespeptides make up proteins; peptoids are molecules closely related to, but more stable than peptides, making them more convenient for testingbut the method could be applied to any class of compound, according to Kodadek.

Changing the Paradigm

The Kodadek group's method combines several different technical advances to enable this convenient and efficient screening.

These days, most active molecules are discovered through screening of two basic types. There are functional screens, in which small molecules are introduced into the wells of microtiter platesflat plates with multiple wells that can reach as high as 9,600and tested individually for their ability to alter the activity of an enzyme. Alternatively, there are binding assays, an approach first developed for bead-displayed peptide libraries, where each bead displays many copies of a single molecule.

"Our new method for screening synthetic libraries and characterizing the resultant hits combines many of the features of bead library screening and microarray-based analysis in a seamless fashion," Kodadek said. "The new technique uses several million beads, each of which displays a unique ligandtheoretically as many as 64
'/>"/>

Contact: Keith McKeown
kmckeown@scripps.edu
858-784-8134
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Scripps scientists discover fluorescence in key marine creature
2. Scripps research team blocks bacterial communication system to prevent deadly staph infections
3. Scripps scientists develop new tests that identify lethal prion strains quickly and accurately
4. Scripps Research discovery leads to broad potential applications in CovX-Pfizer deal
5. Scripps expedition provides new baseline for coral reef conservation
6. Bright lights: Mystery of glowing antibody solved by Scripps research scientists
7. Fishing throws targeted species off balance, Scripps study shows
8. Scripps Oceanography Research pegs ID of red tide killer
9. Scripps Research Institute awarded patent for remarkable chemical technology
10. Scripps research scientists reveal key structure from ebola virus
11. Scripps study sets high economic value on threatened Mexican mangroves
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/17/2014)... Andr Gauthier and Professor Luca Razzari of the ... awarded large grants from the John R. Evans ... (CFI) for the acquisition of state-of-the-art biotech and ... matching grants from the Ministre de l,Enseignement suprieur, ... la Technologie (MESRST). These new laboratories will help ...
(Date:4/17/2014)... to produce hydrogen sulfide in order to properly multiply ... from the Center for Craniofacial Molecular Biology at the ... Songtao Shi, principal investigator on the project, said the ... the flow of calcium ions. The essential ions activate ... or the creation of new bone tissue, and keeps ...
(Date:4/17/2014)... forests in the Amazon help create tinderbox conditions ... and widespread forest loss during drought years, according ... that forests in the Amazon could reach a ... fires lead to large-scale loss of trees, making ... of geography, Penn State. , "We documented one ...
Breaking Biology News(10 mins):New state-of-the-art biotech and nanotech equipment for INRS 2Drought and fire in the Amazon lead to sharp increases in forest tree mortality 2Drought and fire in the Amazon lead to sharp increases in forest tree mortality 3
... MARC (Minority Access to Research Careers) Program has announced the ... of Reproduction (SSR) Annual Meeting in Portland, Oregon from July ... to promote the entry of underrepresented minority students, postdoctorates and ... to encourage the participation of young scientists at the 2011 ...
... lose weight and suspect your body,s working against you, you may ... Obesity . "When obese persons reduce their food intake ... They may have to work harder and go slower in order ... in the U of I College of Medicine and a member ...
... Australia,s fruit and vegetable industry are under threat, ... to find new ways to control a major ... Associate Professor Anthony Clarke, from QUT,s Faculty of ... largest team of university researchers examining non-chemical based ...
Cached Biology News:Obese dieters' brain chemistry works against their weight-loss efforts 2Qld fruit fly scientists in race against time 2Qld fruit fly scientists in race against time 3
(Date:1/14/2014)... January 14, 2014 Carahsoft and CDS ... January 23, 2014 at 2pm EST (11am PST), “Natural ... topic focuses on how technology can turn raw, heterogeneous ... for government agencies. The online webinar will last approximately ...
(Date:1/14/2014)... EquitiesIQ, a leading informational research provider, has ... an emerging biomedical company acquiring, developing, manufacturing, and marketing ... Free report download: http://equitiesiq.com/reports/alliqua/ , In late ... and Board, which launched the company’s new strategy to ...
(Date:1/14/2014)... In recent years, growing suspicion about the pharmaceutical ... and promotion has led to unprecedented levels of public ... about the insidious impact of commercialization of research, has ... the world’s biggest pharmas for illegal marketing activities, allegations ...
(Date:1/14/2014)... and Cardiff, UK (PRWEB) January 13, 2014 ... development leader with more than 20 years in the ... optics and photonics . Hainsey will serve as the ... to have Dr. Hainsey join SPIE as our Technology ...
Breaking Biology Technology:Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Photonics R&D Leader Bob Hainsey Joins SPIE Technical Staff 2
... March 26In a new approach to invisibility cloaking, a team ... sources of heatessentially "thermal cloaking." This method, which the researchers ... Optics Express , taps into some of ... novel ways to control heat in electronics and, on an ...
... IRVINE, Calif., March 26, 2012 Aviir, a biotechnology ... innovative laboratory tests and Guard A Heart, a non-profit ... will sponsor Storyland, a Red Carpet Charity Extravaganza in ... have a whimsical Dr. Seuss theme, as homage to ...
... aura of magic about it because it suddenly reveals what ... So, too, with photoacoustic tomography, which is allowing scientists to ... see what lies beneath. The technique achieves this depth ... the high contrast due to light absorption by colored molecules ...
Cached Biology Technology:Not your average heat shield 2Aviir Diagnostics Laboratory in Collaboration with Cause;ology Sponsors "Storyland," a Red Carpet Charity Extravaganza in Hollywood 2Aviir Diagnostics Laboratory in Collaboration with Cause;ology Sponsors "Storyland," a Red Carpet Charity Extravaganza in Hollywood 3New imaging technique moves from lab to clinic 2New imaging technique moves from lab to clinic 3New imaging technique moves from lab to clinic 4New imaging technique moves from lab to clinic 5
Candida (IgG) -Ab EIA Sample Size: 5 l...
... Oxidized LDL (Competitive) EIA Principle: ... the monoclonal antibody 4E6. Oxidized LDL in the ... LDL bound to the microtiter well for the ... a washing st Sample Size: 25 ...
... used with its manganese-containing reaction buffer, converts ... of short (1825 bp) interfering RNAs (siRNA) ... 1.3 units (1 l) of ShortCut RNase ... of dsRNA into siRNA suitable for RNA ...
For cleavage of 6xHis tags from proteins containing a Factor Xa Protease recognition site Efficient 6xHis tag cleavage (greater than 90%), ...
Biology Products: