JUPITER, FL, March 3, 2011 Borrowing a page from modern manufacturing, scientists from the Florida campus of The Scripps Research Institute have built a microscopic assembly line that mass produces synthetic cell-like compartments.
The new computer-controlled system represents a technological leap forward in the race to create the complex membrane structures of biological cells from simple chemical starting materials.
"Biology is full of synthetic targets that have inspired chemists for more than a century," said Brian Paegel, Scripps Research assistant professor and lead author of a new study published in the Journal of the American Chemical Society. "The lipid membrane assemblies of cells and their organelles pose a daunting challenge to the chemist who wants to synthesize these structures with the same rational approaches used in the preparation of small molecules."
While most cellular components such as genes or proteins are easily prepared in the laboratory, little has been done to develop a method of synthesizing cell membranes in a uniform, automated way. Current approaches are capricious in nature, yielding complex mixtures of products and inefficient cargo loading into the resultant cell-like structures.
The new technology transforms the previously difficult synthesis of cell membranes into a controlled process, customizable over a range of cell sizes, and highly efficient in terms of cargo encapsulation.
The membrane that surrounds all cells, organelles and vesicles small subcellular compartments consists of a phospholipid bilayer that serves as a barrier, separating an internal space from the external medium.
The new process creates a laboratory version of this bilayer that is formed into small, cell-sized compartments.
How It Works
"The assembly-line process is simple and, from a chemistry standpoint, mechanistically clear," said Sandro Matosevic, research as
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Scripps Research Institute