Navigation Links
Scripps Research Institute scientists describe elusive replication machinery of flu viruses
Date:11/22/2012

ample, avian flu viruses from infecting mammals. Mutations at key points on the enzyme have enabled the virus to infect new species in the past. Thus researchers are eager to know the precise details of how the flu polymerase and the rest of the RNP interact.

Getting those details has been a real challenge. One reason is that flu RNPs are complex assemblies that are hard to produce efficiently in the lab. Flu polymerase genes are particularly resistant to being expressed in test cells, and their protein products exist in three separate pieces, or subunits, that have to somehow self-assemble. Until now, the only flu RNPs that have been reproduced in the laboratory are shortened versions whose structures aren't quite the same as those of native flu RNPs. Researchers also are limited in how much virus they can use for such studies.

The team nevertheless managed to develop a test-cell expression system that produced all of the protein and RNA components needed to make full-length flu RNPs. "We were able to get the cells to assemble these components properly so that we had working, self-replicating RNPs," said Robert N. Kirchdoerfer, a first author of the study. Kirchdoerfer was a PhD candidate in the Wilson laboratory during the study, and is now a postdoctoral research associate in the laboratory of TSRI Professor Erica Ollmann Saphire.

Kirchdoerfer eventually purified enough of these flu RNPs for electron microscope analysis at TSRI's Automated Molecular Imaging Group, which is run jointly by Carragher and Potter.

Never Seen Before

The imaging group's innovations enable researchers to analyze molecular samples more easily, in less time, and often with less starting material. "We were able, for example, to automatically collect data for several days in a row, which is unusual in electron microscopy work," said Arne Moeller, a postdoctoral research associate at the imaging group who was the other first author of the
'/>"/>

Contact: Jann Coury
jcoury@scripps.edu
858-784-8245
Scripps Research Institute
Source:Eurekalert  

0

GOOD

Page: 1 2 3

Related biology news :

1. Scripps Research discoveries lead to newly approved drug for infant respiratory distress syndrome
2. Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus
3. Scripps Research Institute Professor Gerald F. Joyce elected to American Academy of Arts & Sciences
4. Scripps Research Institute scientists develop antidote for cocaine overdose
5. Scientist wins $3 million renewal of one of longest-running NIH grants to Scripps Research
6. Scripps research scientists find anticonvulsant drug helps marijuana smokers kick the habit
7. Scripps Florida scientist awarded $1.5 million to design therapeutics with new RNA approach
8. Scripps Florida scientists identify neurotranmitters that lead to forgetting
9. Plastic trash altering ocean habitats, Scripps study shows
10. Scripps Florida scientists awarded $8.4 million grant to develop new anti-smoking treatments
11. Esther B. OKeeffe Foundation gives $2 million to the Scripps Research Institute
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scripps Research Institute scientists describe elusive replication machinery of flu viruses
(Date:5/17/2013)... AGA Research Foundation announced a new grant that intends ... microbiota, one of today,s most exciting areas of science, ... Research Awards Panel selected Andrew T. Chan, MD, MPH ... Medical School, Boston, as the 2013 AGA-Elsevier Gut Microbiome ... of funding, commencing in July 2013, to test the ...
(Date:5/17/2013)... investigating the interaction of a group of proteins in ... have identified a new target that could increase cell ... University,s School of Biochemistry and published in the ... could eventually lead to new therapies for stroke and ... work by the team which identified a protein, known ...
(Date:5/17/2013)... Societies for Experimental Biology (FASEB) released a new ... trends highlighting the devastating impact of sequestration on the ... follows a May 15th Senate Appropriations Committee hearing featuring ... the $1.6 billion cut the agency sustained in FY ... on the scientific community." , According to data ...
Breaking Biology News(10 mins):New gut microbiome research to explore red meat -- colorectal cancer pathway 2SUMO wrestling cells reveal new protective mechanism target for stroke 2
... - Wheat plants found to be resistant to Hessian ... up rigid defenses. Christie Williams, a research scientist ... and a Purdue University associate professor of entomology, found ... increased production of surface waxes and cutin, a molecule ...
... GALVESTON, Texas Why do people become physically weaker ... stop, or even reverse this process, breaking the link between ... online this Wednesday in the Journal of Clinical Endocrinology ... researchers present evidence that answers to both those questions can ...
... MN Bringing the power of synchrotron light to ... aim of a Memorandum of Agreement (MOA) signed today ... national synchrotron facility, the Canadian Light Source (CLS). ... scientific and product outcomes while improving operating efficiency for ...
Cached Biology News:Resistant wheat rebuilds cell walls when attacked by Hessian flies 2Muscle loss in elderly linked to blood vessels' failure to dilate 2BioBusiness Alliance, Canadian Light Source Synchrotron sign agreement 2
(Date:5/17/2013)... German . Photographers ... you can see which branch a bird is sitting on, ... the telephoto lens for high-magnification images requires considerable skill. It ... study proteins, the active biomolecules of cells. , Olga Schubert, ... Biology, and her colleagues have now come up with a ...
(Date:5/17/2013)... USA (PRWEB) May 17, 2013 •    First ... Globes Certified for sustainability ,     New facility will ... , Syngenta unveiled its new ... at the company’s RTP Innovation Center. The first of ... researchers to simulate any agricultural climate and precisely measure ...
(Date:5/17/2013)... -- Yongye International, Inc. (NASDAQ: YONG ) ("Yongye" ... of crop nutrient products in the People,s ... May 16, 2013, the special committee (the "Special Committee") ... was provided a letter (the "Letter") issued by Abax ... ("Abax") to Full Alliance International Limited ("Full Alliance"). The ...
(Date:5/17/2013)... 17, 2013 Men’s skin is biologically different ... Posay ) and has been known to react more aggressively ... may be far from puberty, other acne causing factors like ... breakouts, and threatening skin with unsightly acne scars. Adult acne ... Men’s Health on how to reduce and prevent acne ...
Breaking Biology Technology:Proteome atlas for the tuberculosis pathogen 2Proteome atlas for the tuberculosis pathogen 3Syngenta Opens Unique $72 Million Advanced Crop Lab 2Syngenta Opens Unique $72 Million Advanced Crop Lab 3Yongye International Provides Update on Status of Proposed Go Private Offer 2Yongye International Provides Update on Status of Proposed Go Private Offer 3Yongye International Provides Update on Status of Proposed Go Private Offer 4Adult Acne Treatment, Probiotic Action Shares New Insight on Why Men’s Skin Scars from Acne and How to Prevent It 2
... for Pharmaceutical ... ... Codexis, Inc., a private,biotechnology company and leading developer of clean ... California Institute of Technology for preparing and identifying,drug metabolites (substances ...
... Colo., Aug. 29 AspenBio Pharma,Inc. (Nasdaq: APPY ... novel drugs and diagnostics for animals and humans,reported today ... annual Roth,Capital Partners New York Investor Conference to be ... investors are expected to attend the,conference, which will be ...
... of cardiovascular genetics ... ... GeneNews Limited (TSX: GEN),a company focused on developing patient-friendly blood-based ... or medical,condition, today announced that its co-founder and Chief Scientist ...
Cached Biology Technology:Codexis Licenses New Drug Metabolite Profiling and Lead Diversification Technology From the California Institute of Technology 2AspenBio Pharma to Present at the Roth Capital Partners New York Investor Conference 2AspenBio Pharma to Present at the Roth Capital Partners New York Investor Conference 3GeneNews' Chief Scientist Dr. C.C. Liew co-editor of new genomics reference book 2
Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into tissue culture cells via calcium phosphate co-precipitation....
Request Info...
...
...
Biology Products: