Navigation Links
Scripps Research Institute scientists describe elusive replication machinery of flu viruses
Date:11/22/2012

ample, avian flu viruses from infecting mammals. Mutations at key points on the enzyme have enabled the virus to infect new species in the past. Thus researchers are eager to know the precise details of how the flu polymerase and the rest of the RNP interact.

Getting those details has been a real challenge. One reason is that flu RNPs are complex assemblies that are hard to produce efficiently in the lab. Flu polymerase genes are particularly resistant to being expressed in test cells, and their protein products exist in three separate pieces, or subunits, that have to somehow self-assemble. Until now, the only flu RNPs that have been reproduced in the laboratory are shortened versions whose structures aren't quite the same as those of native flu RNPs. Researchers also are limited in how much virus they can use for such studies.

The team nevertheless managed to develop a test-cell expression system that produced all of the protein and RNA components needed to make full-length flu RNPs. "We were able to get the cells to assemble these components properly so that we had working, self-replicating RNPs," said Robert N. Kirchdoerfer, a first author of the study. Kirchdoerfer was a PhD candidate in the Wilson laboratory during the study, and is now a postdoctoral research associate in the laboratory of TSRI Professor Erica Ollmann Saphire.

Kirchdoerfer eventually purified enough of these flu RNPs for electron microscope analysis at TSRI's Automated Molecular Imaging Group, which is run jointly by Carragher and Potter.

Never Seen Before

The imaging group's innovations enable researchers to analyze molecular samples more easily, in less time, and often with less starting material. "We were able, for example, to automatically collect data for several days in a row, which is unusual in electron microscopy work," said Arne Moeller, a postdoctoral research associate at the imaging group who was the other first author of the
'/>"/>

Contact: Jann Coury
jcoury@scripps.edu
858-784-8245
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Scripps Research discoveries lead to newly approved drug for infant respiratory distress syndrome
2. Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus
3. Scripps Research Institute Professor Gerald F. Joyce elected to American Academy of Arts & Sciences
4. Scripps Research Institute scientists develop antidote for cocaine overdose
5. Scientist wins $3 million renewal of one of longest-running NIH grants to Scripps Research
6. Scripps research scientists find anticonvulsant drug helps marijuana smokers kick the habit
7. Scripps Florida scientist awarded $1.5 million to design therapeutics with new RNA approach
8. Scripps Florida scientists identify neurotranmitters that lead to forgetting
9. Plastic trash altering ocean habitats, Scripps study shows
10. Scripps Florida scientists awarded $8.4 million grant to develop new anti-smoking treatments
11. Esther B. OKeeffe Foundation gives $2 million to the Scripps Research Institute
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scripps Research Institute scientists describe elusive replication machinery of flu viruses
(Date:7/11/2014)... at the Department of Energy,s Oak Ridge National ... by R&D Magazine in recognition of the year,s ... the tremendous value of our National Labs," said ... at the National Labs continues to help our ... scientific and technological innovations necessary to remain globally ...
(Date:7/11/2014)... Bone and Mineral Research awarded Michael F. Holick, PhD, ... the 2014 Louis V. Avioli Award. Holick, a professor ... known for revolutionizing the understanding of vitamin D and ... honors a member of the American Society for Bone ... and mineral basic research. It is named for ASBMR,s ...
(Date:7/11/2014)... Cell scientists have set a "mouse TRAP" to capture ... a recent study published in the Journal of ... a technique called TRAP to extract cellular and genetic ... by scientists at the Rockefeller Institute for Medical Research ... the protein-making machinery, or ribosomes, of the cell type ...
Breaking Biology News(10 mins):ORNL wins eight R&D 100s 2ORNL wins eight R&D 100s 3ORNL wins eight R&D 100s 4BUSM's Michael F. Holick receives American Society for Bone and Mineral Research award 2
... Scientists propose developing an environmentally-friendly fungal spray that would ... a critical growth stage. This spray will contain ... and will have no side-effects on non-target organisms and ... therefore provide a quick, safe and effective means for ...
... the Technion Israel Institute of Technology, the ... have recently identified genes responsible for the positive ... drug Copaxone®. These findings may contribute to the ... Copaxone® was the first original Israeli drug ...
... -- A new book by researchers at the ... the mouse demonstrates a surprising similarity between mice and humans. ... by a team from the Hebrew University Bone Laboratory consisting ... Hajbi-Yonissi and Dr. Yankel Gabet. Also participating in the writing ...
Cached Biology News:New hope for horse lovers as effective control for killer ragwort is proposed 2Israeli scientists identify: Genes that affect responses of multiple sclerosis patients to copaxone 2Of mice and men: similarities between skeletons of both 2
(Date:7/10/2014)... 10, 2014 OMICS Group’s 5th International ... 18-20 August, 2014 at Double Tree by Hilton Beijing, ... developments in Analytical & Bioanalytical research methods only to ... on this occasion, Dr. Srinubabu Gedela, MD of OMICS ... is a remarkable one in bringing a unique and ...
(Date:7/10/2014)... ST. PETERSBURG, Russia , July 10, 2014 /PRNewswire/ ... the Russian Federation Dmitry Medvedev , presented ... a biopharmaceutical company BIOCAD . The Company is ... the scope of MabNext project BIOCAD develops a number ... of cancer and autoimmune diseases. The ceremony took place ...
(Date:7/10/2014)... OAKS, Calif., July 10, 2014   Ceres, Inc ... and seed company, announced today financial results for the ... update on its business. Ceres reported that ... in product performance this growing season in Brazil, which ... affected the company,s sorghum evaluation areas for part of ...
(Date:7/10/2014)... journal PNAS (Proceedings of the National Academy of ... sleep deprivation have a significant effect on our metabolism. ... best time of day to test for diseases such ... effectively. , Researchers from the University of Surrey and ... between sleep deprivation, body clock disruption and metabolism, and ...
Breaking Biology Technology:A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Time of day crucial to accurately test for diseases, new research finds 2
... 19 /PRNewswire-FirstCall/ - ARIUS Research Inc., (TSX:,ARI), a ... of,antibody therapeutics, today announced that David Young, MD, ... at the Chinese Global,Financial Forum. Dr. Young will ... will be available prior to and following his ...
... ... WEST HAVEN, Conn., Oct. 19 Dr. Heather Coyle ... Haven have,developed a new method for preparing certain skeletal remains ... the,research the group is doing for the Smithsonian Institution. Obtaining ...
... Therapeutics Inc., a,Nutritional Therapeutics Innovation Company, today announced ... Wakeman as President. "Pilot has always had ... and dietary supplement areas with over 60 issued ... potential to rapidly move our,world class technologies and ...
Cached Biology Technology:ARIUS to present at Chinese Global Financial Forum 2800-Year-Old Mummies Aid Current Cold Cases: 2Pilot Therapeutics Announces New Executive Team with Extensive Experience Creating New Health and Nutrition Businesses 2
... exciting product for cell-based kinetic assays and ... use of expensive HTS equipment, which lead ... assay development stage. The NOVOstar offers the ... well as any other assay suitable for ...
... measures mitochondria intactness and detects events ... Features and Benefits: Fast, ... isolated mitochondria and for assaying mitochondria ... reagents required for the detection of ...
... save space and organize gloves, ... other garb. They are the ... small parts used in labs, ... Open-slot design for easy cleaning. ...
... handy dispensers save space and ... hats, and other garb. They ... bins for small parts used ... Clear acrylic. Many other sizes ...
Biology Products: