Navigation Links
Scripps Research Institute scientists describe elusive replication machinery of flu viruses
Date:11/22/2012

ample, avian flu viruses from infecting mammals. Mutations at key points on the enzyme have enabled the virus to infect new species in the past. Thus researchers are eager to know the precise details of how the flu polymerase and the rest of the RNP interact.

Getting those details has been a real challenge. One reason is that flu RNPs are complex assemblies that are hard to produce efficiently in the lab. Flu polymerase genes are particularly resistant to being expressed in test cells, and their protein products exist in three separate pieces, or subunits, that have to somehow self-assemble. Until now, the only flu RNPs that have been reproduced in the laboratory are shortened versions whose structures aren't quite the same as those of native flu RNPs. Researchers also are limited in how much virus they can use for such studies.

The team nevertheless managed to develop a test-cell expression system that produced all of the protein and RNA components needed to make full-length flu RNPs. "We were able to get the cells to assemble these components properly so that we had working, self-replicating RNPs," said Robert N. Kirchdoerfer, a first author of the study. Kirchdoerfer was a PhD candidate in the Wilson laboratory during the study, and is now a postdoctoral research associate in the laboratory of TSRI Professor Erica Ollmann Saphire.

Kirchdoerfer eventually purified enough of these flu RNPs for electron microscope analysis at TSRI's Automated Molecular Imaging Group, which is run jointly by Carragher and Potter.

Never Seen Before

The imaging group's innovations enable researchers to analyze molecular samples more easily, in less time, and often with less starting material. "We were able, for example, to automatically collect data for several days in a row, which is unusual in electron microscopy work," said Arne Moeller, a postdoctoral research associate at the imaging group who was the other first author of the
'/>"/>

Contact: Jann Coury
jcoury@scripps.edu
858-784-8245
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Scripps Research discoveries lead to newly approved drug for infant respiratory distress syndrome
2. Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus
3. Scripps Research Institute Professor Gerald F. Joyce elected to American Academy of Arts & Sciences
4. Scripps Research Institute scientists develop antidote for cocaine overdose
5. Scientist wins $3 million renewal of one of longest-running NIH grants to Scripps Research
6. Scripps research scientists find anticonvulsant drug helps marijuana smokers kick the habit
7. Scripps Florida scientist awarded $1.5 million to design therapeutics with new RNA approach
8. Scripps Florida scientists identify neurotranmitters that lead to forgetting
9. Plastic trash altering ocean habitats, Scripps study shows
10. Scripps Florida scientists awarded $8.4 million grant to develop new anti-smoking treatments
11. Esther B. OKeeffe Foundation gives $2 million to the Scripps Research Institute
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scripps Research Institute scientists describe elusive replication machinery of flu viruses
(Date:4/2/2015)... Md. , April 2, 2015 At ... Salt Lake City , the American College ... of five new directors to its Board.  Members of ... the ACMG and for forming and advancing its policies ... medical genetics profession. "It,s an eventful time ...
(Date:4/1/2015)... 1, 2015   Medisafe ™, the leading global ... iOS and Android smartphones and ... into the platform to allow patients to track and ... first time, patients will be able to visualize in ... important biometrics, such as glucose levels and blood pressure. ...
(Date:3/26/2015)... The Granite Club, ... club, today announced it has implemented a state-of-the-art access ... for members and staff, while restricting access to authorized ... selected FST,s IMID Access system because it has the ... and staff, in addition to unparalleled security," said ...
Breaking Biology News(10 mins):American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2
... not affect how long you will live. Instead it is how ... reveals research from the University of Gothenburg recently published in the ... that people with parents who lived to be very old are ... that,s just not true our study shows that hereditary factors ...
... be a problem of towns and cities but new research published ... shows that binge drinking in rural areas is more of ... Psychiatric University Clinic Erlangen, looked at the drinking patterns of over ... more than 93% of the young people from the countryside and ...
... Kyoto, Japan -- In a complex feat of ... the University of Oxford have succeeded in creating a ... be observed in real time. The results, appearing in ... the door to the development of advanced drug delivery ...
Cached Biology News:Lifestyle affects life expectancy more than genetics 2
(Date:4/20/2015)... , April 20, 2015 PointCross Life ... Ltd. to provide an integrated Big Data and globally ... biomarkers collected in the context of clinical trials and ... precise or multi-targeted drug candidates, stratified patient selection and ... progression free survival. Highlighting the timing ...
(Date:4/20/2015)... ANGELES , April 20, 2015  "Everybody is ... we do to patients is frankly brutal. We,ve got ... in a cancer ward, or witnessed a loved one ... you,ve witnessed firsthand the devastation chemotherapy and other treatments ... most people want to give up and succumb to ...
(Date:4/20/2015)... 2015 /CNW/ - Portage Biotech Inc. ("Portage" or "the ... pleased to announce that its wholly owned subsidiary, Portage ... that showed one of its proprietary human-derived CPP sequences ... through inhibition of NFkB signalling even if administered when ... of the blood brain barrier (BBB) in mice was ...
(Date:4/20/2015)... 20, 2015   Meditope Biosciences, Inc ., a ... technology, today announced presentation of data demonstrating the use ... antibody-drug conjugates (ADCs). The data were presented in an abstract ... 18-22 in Philadelphia . ... point to the many commercial applications that may be ...
Breaking Biology Technology:PointCross Life Sciences and InterpretOmics partner for Clinical and Genomic Biomarker Management for Drug Development 2Is Dr. Craig Dionne the Steve Jobs of Cancer Research? 2Portage's CPP Platform Delivers a Peptide Cargo Across Blood Brain Barrier 2Portage's CPP Platform Delivers a Peptide Cargo Across Blood Brain Barrier 3Meditope Biosciences Presents Data At The American Association Of Cancer Research (AACR) Annual Meeting Validating Its SnAP Technology Platform 2
... IL - SecurePipe , a provider of ... an agreement to acquire the assets of Mycom's ... management technologies from Cincinnati-based Mycom Group, Inc. , ,SecurePipe ... sectors such as manufacturing, education, health care and financial ...
... Madison, Wis. - R&D Magazine has ... a 2005 R&D 100 Award, which recognizes the most ... past year. , ,The VANTEC-2000 system is an advanced ... science, materials science and nanotechnology research fields. It enables ...
... been utilized by thousands of U.S. companies since the ... fact, lawmakers in more than 35 states have introduced ... as an effective cost-cutting tool has begun to soar. ... offshore outsourcing, it was often viewed as ...
Cached Biology Technology:Consider the legal issues before outsourcing offshore 2Consider the legal issues before outsourcing offshore 3Consider the legal issues before outsourcing offshore 4Consider the legal issues before outsourcing offshore 5
... amber to slightly cloudy liquid. Yellow, ... per set, each containing 10 ml ... the following proportions by weight: 85% ... monooleate emulsifier), and 0.1% Mycobacterium ...
... been many modifications to the original formulas in ... still play an important role in tissue,culture. A ... and osmotic balance in the medium and to ... ions, is as valuable today as when it ...
Request Info...
... ion trap mass spectrometer enables high-throughput solutions ... LXQ linear ion trap mass spectrometer is ... delivering the fast cycle time and increased ... at an attractive price. The combination ...
Biology Products: