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Scripps Florida scientists offer 'best practices' nutrition measurement for researchers
Date:3/30/2014

JUPITER, FL, March 30, 2014 At first glance, measuring what the common fruit fly eats might seem like a trivial matter, but it is absolutely critical when it comes to conducting studies of aging, health, metabolism and disease. How researchers measure consumption can make all the difference in the accuracy of a study's conclusions.

Scientists from the Florida campus of The Scripps Research Institute (TSRI) have developed what amounts to a best practices guide to the most accurate way of measuring fruit fly food consumption that could lead to more informed research and better decisions about directions in further studies.

"While our study isn't the final technical reference on measuring fly food consumption, it will help guide researchers to think more carefully about nutrition and nutrient intake in their own studies," said TSRI Assistant Professor William Ja, who led the study, which was published online ahead of print on March 30, 2014 by the journal Nature Methods.

Researchers, Ja said, generally haven't given sufficient thought to feeding and nutrient intake when it comes to measuring fruit fly behavior, metabolism and health.

"If you're making a huge effort to change an animal's diet and trying to draw conclusions about what nutrition and nutrients do to animal health and lifespan," he said, "then one of the most fundamental parameters is accurately measuring food intake."

TSRI Research Associate Sonali Deshpande, a first author of the study with graduate student Ariadna Amador and former TSRI Research Associate Gil Carvalho, underlined the importance of using the best measurement methods. "Drug studies, in particular, where compounds are added to fly food, are difficult to interpret without proper measurement of food and drug intake," she said.

In the study, the team determined that radioisotope labeling food is the most sensitive and consistently accurate feeding method now availablelevels of
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Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

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