Navigation Links
Scripps Florida scientists offer 'best practices' nutrition measurement for researchers
Date:3/30/2014

JUPITER, FL, March 30, 2014 At first glance, measuring what the common fruit fly eats might seem like a trivial matter, but it is absolutely critical when it comes to conducting studies of aging, health, metabolism and disease. How researchers measure consumption can make all the difference in the accuracy of a study's conclusions.

Scientists from the Florida campus of The Scripps Research Institute (TSRI) have developed what amounts to a best practices guide to the most accurate way of measuring fruit fly food consumption that could lead to more informed research and better decisions about directions in further studies.

"While our study isn't the final technical reference on measuring fly food consumption, it will help guide researchers to think more carefully about nutrition and nutrient intake in their own studies," said TSRI Assistant Professor William Ja, who led the study, which was published online ahead of print on March 30, 2014 by the journal Nature Methods.

Researchers, Ja said, generally haven't given sufficient thought to feeding and nutrient intake when it comes to measuring fruit fly behavior, metabolism and health.

"If you're making a huge effort to change an animal's diet and trying to draw conclusions about what nutrition and nutrients do to animal health and lifespan," he said, "then one of the most fundamental parameters is accurately measuring food intake."

TSRI Research Associate Sonali Deshpande, a first author of the study with graduate student Ariadna Amador and former TSRI Research Associate Gil Carvalho, underlined the importance of using the best measurement methods. "Drug studies, in particular, where compounds are added to fly food, are difficult to interpret without proper measurement of food and drug intake," she said.

In the study, the team determined that radioisotope labeling food is the most sensitive and consistently accurate feeding method now availablelevels of accumulated isotope are later measured in the animals. This method's main limitation appears to be underestimation of consumption due to excretion.

For the most accurate measurement, the study suggested pairing radioisotope labeling with a more low-tech approach, such as the capillary feeder (CAFE). The CAFE assay, introduced by Ja in 2007, is similar to a water dispenser used for pet hamsters, but on a smaller scale.

"In a significant number of studies, we found that researchers appeared indifferent to the impact feeding might have on the experiment," Ja said. "This doesn't seem like good science to me. Can you imagine doing a mouse experiment, saying that you watched mice for four hours and saw no difference in feeding, then make conclusions about total caloric intake over days or longer?"


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

Related biology news :

1. NIH grants up to $28 million to group led by Scripps Research for work on ebola treatment
2. Scripps Wellderly Genome Resource now available to researchers
3. New probes from Scripps research quantify folded and misfolded protein levels in cells
4. Scripps scientist awarded $1.8 million to develop new approaches to lung cancer therapy
5. Scripps Florida scientists offer new insight into neuron changes brought about by aging
6. Scripps leads first global snapshot of key coral reef fishes
7. Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
8. Scripps oceanography researchers engineer breakthrough for biofuel production
9. NIH awards Scripps Translational Science Institute $29 million grant
10. James Cameron to be publicly honored with Scripps Nierenberg Prize
11. Scripps Research announces research & license agreement with Janssen Pharmaceuticals
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/4/2017)... , Jan. 4, 2017  CES 2017 – ... biometric sensor technology, today announced the launch of ... sensor systems, the highly-accurate biometric sensor modules that ... biometric technology, experience and expertise. The two new ... designed specifically for hearables, and Benchmark BW2.0, a ...
(Date:12/22/2016)... 2016 SuperCom (NASDAQ:   ... the e-Government, Public Safety, HealthCare, and Finance sectors announced today that ... selected to implement and deploy a community-based supportive services program to ... , further expanding its presence in the state. ... This new program, which is ...
(Date:12/19/2016)... , 19 de diciembre de 2016  Mosaic Biomedicals SL anunció ... acelerado de MSC-1, un anticuerpo humanizado que se espera comenzar a ... con múltiples sitios previstos a lo largo de Europa y Norteamérica. ... MSC-1 es el ... de leucemia (LIF), una citoquina pleiotrópica que se sobreexpresa en ciertos ...
Breaking Biology News(10 mins):
(Date:1/13/2017)... ... January 13, 2017 , ... ... containing an organic compound called fulvic acid that farms, greenhouses and hydroponics operations ... grow cannabis are among the fastest growing segments of customers using this high ...
(Date:1/12/2017)... ... January 12, 2017 , ... ... IS A SUCCESS , VTI, Vertebral Technologies, Inc., announces the successful outcome ... expandable device. Since September 2016, VTI (Vertebral Technologies, Inc.) has partnered with ...
(Date:1/12/2017)... ... ... Huffman Engineering, Inc. , a leader in control systems integration, today announced ... as a chemical engineer. In his new role, Beck will use his extensive ... manufacturing and water/wastewater industries. , Prior to joining Huffman Engineering, Beck served for nine ...
(Date:1/12/2017)... 2017   Protein Sciences Corporation , a ... Flublok Influenza Vaccine ®, announced today that its ... safety results and induced strong neutralizing antibodies against ... is expected to advance into human clinical trials ... Institute of Technology in Immunobiologicals of the Oswaldo ...
Breaking Biology Technology: