Navigation Links
Scripps Florida scientists offer 'best practices' nutrition measurement for researchers
Date:3/30/2014

JUPITER, FL, March 30, 2014 At first glance, measuring what the common fruit fly eats might seem like a trivial matter, but it is absolutely critical when it comes to conducting studies of aging, health, metabolism and disease. How researchers measure consumption can make all the difference in the accuracy of a study's conclusions.

Scientists from the Florida campus of The Scripps Research Institute (TSRI) have developed what amounts to a best practices guide to the most accurate way of measuring fruit fly food consumption that could lead to more informed research and better decisions about directions in further studies.

"While our study isn't the final technical reference on measuring fly food consumption, it will help guide researchers to think more carefully about nutrition and nutrient intake in their own studies," said TSRI Assistant Professor William Ja, who led the study, which was published online ahead of print on March 30, 2014 by the journal Nature Methods.

Researchers, Ja said, generally haven't given sufficient thought to feeding and nutrient intake when it comes to measuring fruit fly behavior, metabolism and health.

"If you're making a huge effort to change an animal's diet and trying to draw conclusions about what nutrition and nutrients do to animal health and lifespan," he said, "then one of the most fundamental parameters is accurately measuring food intake."

TSRI Research Associate Sonali Deshpande, a first author of the study with graduate student Ariadna Amador and former TSRI Research Associate Gil Carvalho, underlined the importance of using the best measurement methods. "Drug studies, in particular, where compounds are added to fly food, are difficult to interpret without proper measurement of food and drug intake," she said.

In the study, the team determined that radioisotope labeling food is the most sensitive and consistently accurate feeding method now availablelevels of accumulated isotope are later measured in the animals. This method's main limitation appears to be underestimation of consumption due to excretion.

For the most accurate measurement, the study suggested pairing radioisotope labeling with a more low-tech approach, such as the capillary feeder (CAFE). The CAFE assay, introduced by Ja in 2007, is similar to a water dispenser used for pet hamsters, but on a smaller scale.

"In a significant number of studies, we found that researchers appeared indifferent to the impact feeding might have on the experiment," Ja said. "This doesn't seem like good science to me. Can you imagine doing a mouse experiment, saying that you watched mice for four hours and saw no difference in feeding, then make conclusions about total caloric intake over days or longer?"


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

Related biology news :

1. NIH grants up to $28 million to group led by Scripps Research for work on ebola treatment
2. Scripps Wellderly Genome Resource now available to researchers
3. New probes from Scripps research quantify folded and misfolded protein levels in cells
4. Scripps scientist awarded $1.8 million to develop new approaches to lung cancer therapy
5. Scripps Florida scientists offer new insight into neuron changes brought about by aging
6. Scripps leads first global snapshot of key coral reef fishes
7. Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
8. Scripps oceanography researchers engineer breakthrough for biofuel production
9. NIH awards Scripps Translational Science Institute $29 million grant
10. James Cameron to be publicly honored with Scripps Nierenberg Prize
11. Scripps Research announces research & license agreement with Janssen Pharmaceuticals
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/30/2017)... June 30, 2017 Today, American Trucking ... supplier of face and eye tracking software, became ... provider program. "Artificial intelligence and ... to monitor a driver,s attentiveness levels while on ... able to detect fatigue and prevent potential accidents, ...
(Date:6/14/2017)... 15, 2017  IBM (NYSE: IBM ) is introducing several ... dedicated to developing collaboration between startups and global businesses, taking ... During the event, nine startups will showcase the solutions they ... industries. France is ... with a 30 percent increase in the number of startups ...
(Date:5/16/2017)... N.J. , May 16, 2017  Veratad Technologies, ... provider of online age and identity verification solutions, announced ... K(NO)W Identity Conference 2017, May 15 thru May 17, ... Regan Building and International Trade Center. ... the globe and in today,s quickly evolving digital world, ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... , ... October 11, 2017 , ... ... the implantation and pregnancy rates in frozen and fresh in vitro fertilization ... progesterone and maternal age to IVF success. , After comparing the results from ...
(Date:10/10/2017)... , ... October 10, 2017 ... ... cancer-focused pharmaceutical company advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing ... HPLN (Hybrid Polymerized Liposomal Nanoparticle), a technology developed in collaboration with Children’s ...
(Date:10/10/2017)... research firm Parks Associates announced today that Tom Kerber ... Annual Meeting , October 11 in Scottsdale, Arizona . ... how smart safety and security products impact the competitive landscape. ... Parks Associates: Smart Home Devices: Main Purchase Driver ... "The residential security market has experienced continued growth, and the ...
(Date:10/10/2017)... , ... October 10, 2017 , ... ... of 13 prestigious awards honoring scientists who have made outstanding ... a scheduled symposium during Pittcon 2018, the world’s leading conference and exposition for ...
Breaking Biology Technology: