Navigation Links
Scientists unravel the genetic coding of the pea
Date:2/25/2008

The pea is one of many important crop species that is unsuited to the Agrobacterium-based genetic modification techniques that are commonly used to work with crops. Researchers, reporting in the open access journal Genome Biology have now discovered the first high-throughput forward and reverse genetics tool for the pea (Pisum sativum), could have major benefits for crop breeders around the world..

Researchers from the INRA Plant Genomics Research Unit at Evry, and the INRA Grain Legumes Research Unit at Bretenires, both in France, both in France developed a high-quality genetic reference collection of Pisum sativum mutants within the European Grain Legumes Integrated Project. Abdelhafid Bendahmane and colleagues used plants from an early-flowering garden pea cultivar, Camor, to create a mutant population, which they then systematically phenotyped for use in both forward and reverse genetics studies.

The team set up a pea TILLING (Targeting Induced Local Lesions IN Genomes) platform with DNA samples from 4,704} plants. The TILLING technique overcomes the peas natural unsuitability to genetic modification techniques, and provides a powerful tool for investigating the role of essential genes. This new tool has implications for both basic science and for crop improvement. TILLING is an alternative to Agrobacterium-based techniques, and uses EMS (ethane methyl sulfonate) mutagenesis coupled with a gene-specific detection of single-nucleotide mutations. This reverse genetic strategy can be applied to all types of organisms and can be automated for high-throughput approaches. Following this study, the researchers created a database called UTILLdb, which described each mutant plant at different developmental stages, (from seedling through to fruit maturation), and also incorporates digital images of the plants. UTILLdb contains phenotypic as well as sequence information on mutant genes, and can be searched for TILLING alleles of genes of interest, using the BLAST tool, and for plant traits of interest, using keyword searches.

By opening UTILLdb to the community, we hope to fulfil the expectations of both crop breeders and scientists who are using the pea as their model of study, said research coordinator Abdelhafid Bendahmane.


'/>"/>

Contact: Charlotte Webber
press@biomedcentral.com
44-020-763-19980
BioMed Central
Source:Eurekalert

Related biology news :

1. Scientists discover giant fossil frog from hell
2. Advertisers, neuroscientists trace source of emotions in brain
3. Amazon corridors far too narrow, warn scientists
4. Dung happens and helps scientists
5. Priming scientists for successful media interviews
6. Scientists expand understanding of how river carbon impacts the Arctic Ocean
7. Fishermen and UCSB scientists explore ways to improve management of California spiny lobsters
8. Scientists rebuild ancient proteins to reveal primordial Earths temperature
9. Rice scientists make breakthrough in single-molecule sensing
10. Carnegie Mellon scientists develop fluorescent proteins for live cell imaging, biosensor design
11. Scientists obtain core samples from subsea fault system off Japan
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/14/2016)... 14, 2016  xG Technology, Inc. ("xG" or the ... wireless communications for use in challenging operating environments, announced ... 2016. Management will hold a conference call to discuss ... Eastern Time (details below). Key Recent Accomplishments ... million binding agreement to acquire Vislink Communication Systems. The ...
(Date:6/22/2016)... BETHESDA, Md. , June 22, 2016  The American ... by Trade Show Executive Magazine as one of ... Summit on May 25-27 at the Bellagio in ... based on the highest percentage of growth in each of ... number of exhibiting companies and number of attendees. The 2015 ...
(Date:6/21/2016)... VANCOUVER, British Columbia , June 21, 2016 ... been appointed to the new role of principal ... has been named the director of customer development. ... , NuData,s chief technical officer. The moves reflect ... development teams in response to high customer demand ...
Breaking Biology News(10 mins):
(Date:12/5/2016)... ... December 05, 2016 , ... In anticipation of AxioMed’s exclusive ... production, company President, Jake Lubinski will be traveling to Germany on December 6th. ... Cologne and Karlsruhe to discuss the benefits of a viscoelastic total disc replacement. ...
(Date:12/5/2016)... , Dec. 5, 2016  Eisai Inc. ... open-label two-year study of rufinamide, which were presented ... Epilepsy Society (AES) held from December 2-6 in ... final two-year safety, tolerability and cognitive data showed ... experienced similar safety and tolerability profiles, cognitive development ...
(Date:12/4/2016)... 2016  In five studies being presented today during ... and Exposition in San Diego , ... the delivery of life-saving treatments to patients with a ... to carry therapies directly to the sites in the ... a substantial advantage over traditional, systemic methods. The studies ...
(Date:12/2/2016)... Amgen (NASDAQ: AMGN ) and Allergan ... of a Marketing Authorization Application (MAA) to the European Medicines ... ® (bevacizumab). The companies believe this submission is the ... "The submission of ABP 215 to the EMA is ... portfolio," said Sean E. Harper , M.D., executive vice ...
Breaking Biology Technology: