Navigation Links
Scientists unravel the genetic coding of the pea
Date:2/25/2008

The pea is one of many important crop species that is unsuited to the Agrobacterium-based genetic modification techniques that are commonly used to work with crops. Researchers, reporting in the open access journal Genome Biology have now discovered the first high-throughput forward and reverse genetics tool for the pea (Pisum sativum), could have major benefits for crop breeders around the world..

Researchers from the INRA Plant Genomics Research Unit at Evry, and the INRA Grain Legumes Research Unit at Bretenires, both in France, both in France developed a high-quality genetic reference collection of Pisum sativum mutants within the European Grain Legumes Integrated Project. Abdelhafid Bendahmane and colleagues used plants from an early-flowering garden pea cultivar, Camor, to create a mutant population, which they then systematically phenotyped for use in both forward and reverse genetics studies.

The team set up a pea TILLING (Targeting Induced Local Lesions IN Genomes) platform with DNA samples from 4,704} plants. The TILLING technique overcomes the peas natural unsuitability to genetic modification techniques, and provides a powerful tool for investigating the role of essential genes. This new tool has implications for both basic science and for crop improvement. TILLING is an alternative to Agrobacterium-based techniques, and uses EMS (ethane methyl sulfonate) mutagenesis coupled with a gene-specific detection of single-nucleotide mutations. This reverse genetic strategy can be applied to all types of organisms and can be automated for high-throughput approaches. Following this study, the researchers created a database called UTILLdb, which described each mutant plant at different developmental stages, (from seedling through to fruit maturation), and also incorporates digital images of the plants. UTILLdb contains phenotypic as well as sequence information on mutant genes, and can be searched for TILLING alleles of genes of interest, u
'/>"/>

Contact: Charlotte Webber
press@biomedcentral.com
44-020-763-19980
BioMed Central
Source:Eurekalert

Page: 1 2

Related biology news :

1. Scientists discover giant fossil frog from hell
2. Advertisers, neuroscientists trace source of emotions in brain
3. Amazon corridors far too narrow, warn scientists
4. Dung happens and helps scientists
5. Priming scientists for successful media interviews
6. Scientists expand understanding of how river carbon impacts the Arctic Ocean
7. Fishermen and UCSB scientists explore ways to improve management of California spiny lobsters
8. Scientists rebuild ancient proteins to reveal primordial Earths temperature
9. Rice scientists make breakthrough in single-molecule sensing
10. Carnegie Mellon scientists develop fluorescent proteins for live cell imaging, biosensor design
11. Scientists obtain core samples from subsea fault system off Japan
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/11/2014)... July 11, 2014 Research and Markets ... Biometrics Technology Market - Industry Analysis Size Share Growth ... offering. http://photos.prnewswire.com/prnh/20130307/600769 ... has created a need for high level security in ... personnel authentication such as username and passwords, tokens etc. ...
(Date:7/10/2014)... 27, 2014  Pomerantz LLP has filed a ... or the "Company")(NYSE-MKT: PVCT) and certain of its ... District Court, Middle District of Tennessee ... a class consisting of all persons or entities ... December 17, 2013 and May 22, 2014, both ...
(Date:7/10/2014)... SHELTON, Conn. , July 2, 2014 NXT-ID, Inc., ... market, reports that CEO Mr. Gino Pereira was ... radio show in Tampa Florida . Mr. ... radio host Cedric Harris and how the Wocket™ ... secure alternative.  Cedric Harris tells ...
Breaking Biology News(10 mins):International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4
... world-leading provider of scientific, technical and medical information products and ... the European Menopause and Andropause Society (EMAS) in journal ... women with summary recommendations. Vitamin D deficiency is common ... classified as a public health issue as it can contribute ...
... it comes to conserving the world,s orchids, not all forests ... in the journal Molecular Ecology , Smithsonian ecologists revealed ... age and its fungi. Roughly 10 percent of all ... on Earth. But habitat loss has rendered many threatened or ...
... 2010, an entomologist at the University of California, Riverside discovered a ... seen in the United States until then. Nearly exactly a ... that the wasp is well established in the country. Called ... and arrived in North America from Europe. It lays its ...
Cached Biology News:Elsevier's Maturitas publishes position statement on the role of vitamin D in postmenopausal women 2Fungi-filled forests are critical for endangered orchids 2Wasp found in upstate New York shows up in Southern California 2
(Date:7/10/2014)... University of Adelaide research may help in the fight ... can detect tiny quantities of explosives with the use ... the journal Sensors and Actuators B: Chemical , ... can detect explosives in concentrations as low as 6.3 ... of only a few minutes. , "Traditionally explosives detection ...
(Date:7/10/2014)... researchers have observed the fractional quantum Hall effect in ... matter can be tuned by an electric field. ... when electrons confined to thin sheets are exposed to ... behavior where thousands of individual electrons behave as a ... effect is well established, many details of this collective ...
(Date:7/10/2014)... by researchers at the University of Illinois at ... governing the interplay of spin and heat at ... ultrafast spintronic devices for data storage and information ... well as spin-angular momentum. In a typical charge ... no spin current," explained David Cahill, a professor ...
(Date:1/15/2014)... 2014  Bill Jacobs Automotive, a group of car dealerships headquartered ... the Heartland Blood Center and offering free oil change coupons ... place Saturday, Jan. 18 at the Bill Jacobs Cadillac and ... (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The Bill Jacobs ...
Breaking Biology Technology:Detecting trace amounts of explosives with light 2Columbia researchers observe tunable quantum behavior in bilayer graphene 2Columbia researchers observe tunable quantum behavior in bilayer graphene 3University of Illinois study advances limits for ultrafast nano-devices 2Join the Bill Jacobs Auto blood drive and receive a free oil change 2
... -- On March 6, the Air Force Office ... by Dr. Chad Mirkin from Northwestern University. Professor ... who is known for his development of nanoparticle-based biodetection ... Dip-Pen,Nanolithography (DPN), and his contributions to supramolecular chemistry, nanoelectronics, ...
... AlphaVax, Inc. ("AVX") is pleased to report that it has been ... Award as the Best Early-Stage Vaccine Biotech Company. The award will ... in April. AVX is pleased to be both ... of judges as well as being recognized as a leader in ...
... DIEGO, March 20, 2012   CareFusion announced today ... Food and Drug Administration (FDA) for its new electrodiagnostic ... EDX system is a multi-modality device for monitoring and ... system. (Logo: http://photos.prnewswire.com/prnh/20100706/CAREFUSIONLOGO ) ...
Cached Biology Technology:Air Force Office of Scientific Research hosts nanotechnology pioneer 2Air Force Office of Scientific Research hosts nanotechnology pioneer 3Air Force Office of Scientific Research hosts nanotechnology pioneer 4CareFusion Announces 510(k) Clearance and U.S. Launch of New Advanced Electrodiagnostic System 2
Yellow oily liquid. Contains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml. ...
... 470 nm; includes interface cable to S2000; can ... rack or desktop boxes The LS-450 ... that produces either pulsed or continuous output at ... fluorescence measurements. The source is designed for use ...
... Array LNA microarray slides for microRNA ... slides incorporate Exiqons unique Locked Nucleic Acid ... excellent mismatch discrimination for short microRNA (miRNA) ... than 1 g total RNA (no need ...
... to slightly cloudy liquid. Yellow, oily, ... set, each containing 10 ml of ... following proportions by weight: 85% Drakeol ... emulsifier), and 0.1% Mycobacterium butyricum ...
Biology Products: