Navigation Links
Scientists solve long-standing plant biochemistry mystery
Date:9/19/2011

act with the substrate, based on their positions in the structural models.

The scientists identified one position, far from the active site, where the computer model indicated that switching a single amino acid would change the orientation of the bound fatty acid with respect to the active site. Could this distant amino-acid location remotely control the site of double bond placement?

To test this hypothesis, the scientists engineered a new desaturase, swapping out the aspartic acid normally found at that location in the delta-9 castor desaturase for the lysine found in the delta-4 ivy desaturase. The result: an enzyme that was castor-like in every way, except that it now seemed able to desaturate the fatty acid at the delta-4 carbon location. "It's quite remarkable to see that changing just one amino acid could have such a striking effect," Shanklin said.

The computational modeling helped explain why: It showed that the negatively charged aspartic acid in the castor desaturase ordinarily repels a negatively charged region on the carrier protein, which leads to a binding orientation that favors delta-9 desaturation; substitution with positively charged lysine results in attraction between the desaturase and carrier protein, leading to an orientation that favors delta-4 desaturation.

Understanding this mechanism led Ed Whittle, a research associate in Shanklin's lab, to add a second positive charge to the castor desaturase in an attempt to further strengthen the attraction. The result was a nearly complete switch in the castor enzyme from delta-9 to delta-4 desaturation, adding compelling support for the remote control hypothesis.

"I really admire Ed's persistence and insight in taking what was already a striking result and pushing it even further to completely change the way this enzyme functions," Shanklin said.

"It's very rewarding to have finally solved this mystery, which would not have been possible without
'/>"/>

Contact: Karen McNulty Walsh
kmcnulty@bnl.gov
631-344-8350
DOE/Brookhaven National Laboratory
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Online gamers succeed where scientists fail, opening door to new AIDS drug design
2. Gamers succeed where scientists fail
3. Climate scientists will shed new light on climate change mitigation
4. Sea smarts: Scientists reconstruct evolutionary history of mollusks
5. TUM scientists document aquatic species decline at dams and weirs
6. Scientists concerned about pesticide education funding
7. Scientists utilise breath and sweat to detect trapped humans
8. USC scientists generate first detailed map of human neuroreceptor
9. USC scientists probe connection between sight and touch in the brain
10. Biomedical/health informatics scientists to present cutting-edge science, health IT
11. Scientists offer way to address age-old questions
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/28/2014)... 2014A new method for measuring and imaging how quickly ... researchers better understand how drug abuse affects the brain, ... engineering, and lead to better treatment options for recovering ... of researchers from Stony Brook University in New York, ... published today in The Optical Society,s (OSA) open-access journal ...
(Date:8/28/2014)... 25 million kilometres of new roads will be built ... into Earth,s last wildernesses, where they bring an influx ... Now, an ambitious study has created a ,global roadmap, ... to balance the competing demands of development and environmental ... layer that estimates that natural importance of ecosystems and ...
(Date:8/28/2014)... pose a significant health risk to people with asthma according ... Allergy and Clinical Immunology . , By critically reviewing the ... has found that the presence of several types of mould ... as increasing the likelihood of developing the condition. , The ... of Exeter Medical School and is the first time all ...
Breaking Biology News(10 mins):This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3Study shows where on the planet new roads should and should not go 2Study shows where on the planet new roads should and should not go 3Indoor mold poses health risk to asthma sufferers 2
... might not know what it,s called, but if you,ve ... it is likely you have received Sellick,s maneuver. That,s ... prevent regurgitation and spilling of stomach contents into the ... Such regurgitation could result in serious lung damage and ...
... researchers from the United States, Korea, and France has sequenced ... E. coli bacteria, one used to study evolution ... practical applications. The findings will help guide future research and ... classical research that is the foundation of our understanding of ...
... New research released today is affirming a long-held maxim: you ... what you eat has a profound influence on the brain. ... obesity epidemic in the United States and other developed countries. ... and weight gain, and were reported at Neuroscience 2009, the ...
Cached Biology News:UF scientists discover new explanation for controversial old patient-care technique 2UF scientists discover new explanation for controversial old patient-care technique 3Genomes of 2 popular research strains of E. coli sequenced 2Genomes of 2 popular research strains of E. coli sequenced 3Think what you eat: Studies point to cellular factors linking diet and behavior 2
(Date:8/28/2014)... DIEGO, California, USA (PRWEB) August 28, 2014 ... the Department of Physics and Optical Science and Director ... the University of North Carolina at Charlotte, and co-founder ... been elected to serve as the 2015 Vice President ... , SPIE 2014 President Philip Stahl announced recent election ...
(Date:8/28/2014)... in spintronics, an electronic which is not only ... on their spin and the spin-related magnetism. Spin-charge ... signals and vice versa. Recently, the research group ... Physics at Johannes Gutenberg University Mainz in collaboration ... has for the first time realised a new, ...
(Date:8/28/2014)... 28, 2014 Sterlitech is proud to ... to its stable of products . These ... cut-offs and include membrane filters with surface charges. ... customer demand for our membrane process testing equipment, and ... flat sheet membranes,” explains Sterlitech President Mark Spatz. ...
(Date:8/28/2014)... Earlier this month, a report was created by ... Air Force on the performance of vehicles using an ... of fuel made from mustard seeds, offered great potential ... Eco friendly future. In fact, every branch of the ... of foreign oil over the next few years. They ...
Breaking Biology Technology:Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2A new, tunable device for spintronics 2A new, tunable device for spintronics 3Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 3Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 4Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 5
... MSC Care Management, the industry,s leading provider of ... workers, compensation claimants nationwide, announced today that it ... also focused on the workers, compensation industry, to ... management solution, known as S3. Integrated Healthcare Services, ...
... BETHESDA, Md., Oct. 4, 2011 Northwest Biotherapeutics ... will be presenting at the second annual Cancer ... 6, at the New York Academy of Medicine ... key opinion leaders, industry executives, and other stakeholders ...
... Oct. 4, 2011 Kylin Therapeutics, Inc., a ... has received a notice of allowance from United ... second U.S. patent application. This newly allowed patent ... as expanded areas on Kylin,s proprietary nanoparticle technology ...
Cached Biology Technology:MSC to Offer Surgical Implant Cost Management Solution through Acquisition of Integrated Healthcare Services 2NORTHWEST BIO Will Present at the Second Annual "Cancer Immunotherapy: A Long Awaited Reality Conference" at the New York Academy of Medicine, October 6, 2011 2NORTHWEST BIO Will Present at the Second Annual "Cancer Immunotherapy: A Long Awaited Reality Conference" at the New York Academy of Medicine, October 6, 2011 3NORTHWEST BIO Will Present at the Second Annual "Cancer Immunotherapy: A Long Awaited Reality Conference" at the New York Academy of Medicine, October 6, 2011 4Kylin Therapeutics Receives Notice of Allowance for Second U.S. Patent Covering Delivery of Therapeutic RNA and pRNAi Technology 2