Navigation Links
Scientists observe single gene activity in living cells
Date:4/21/2011

April 21, 2011 − (BRONX, NY) − Researchers at Albert Einstein College of Medicine of Yeshiva University have for the first time observed the activity of a single gene in living cells. In an unprecedented study, published in the April 22 online edition of Science, Einstein scientists were able to follow, in real time, the process of gene transcription, which occurs when a gene converts its DNA information into molecules of messenger RNA (mRNA) that go on to make the protein coded by the gene.

Robert Singer, Ph.D., co-director of the Gruss Lipper Biophotonics Center at Einstein and professor and co-chair of anatomy and structural biology, is senior author of the paper. The study's lead author is Daniel Larson, Ph.D., previously a member of Dr. Singer's lab and now an investigator at the National Cancer Institute and head of the institute's Systems Biology of Gene Expression Section.

Using florescent proteins, the researchers were able to follow mRNA activity by inserting DNA sequences into a gene in live yeast cells. RNA made from these sequences bound a modified green fluorescent protein; expression of the entire gene resulted in mRNA molecules that were visible with fluorescent light. Gene transcription is a key step in synthesizing proteins, which govern the body's structure and function and underlie many diseases when present in mutated form or in aberrant amounts.

The study involved monitoring the activity of RNA polymerasethe enzyme that constructs mRNA molecules by linking single nucleotides together into a molecular chain. The researchers were able to directly observe and measure the key steps involved in transcription: initiation (triggered when proteins called transcription factors bind to the promoter region of the gene), elongation (the progressive lengthening of mRNA as RNA polymerase adds more nucleotides to it) and termination (when mRNA breaks free from its DNA template, eventually migrating from the cell nucleus to the cytoplasm to serve as a blueprint for protein construction).

"The view of transcription in yeast that emerges from this study is that its initiation seems to be a random event that depends on the success of transcription factors searching through the yeast nucleus looking for a particular gene's promoter region," said Dr. Singer. "By contrast, once initiation occurs, RNA polymerase recruits individual nucleotides for the growing mRNA molecule in an efficient and predictable manner."

"Understanding how gene expression is regulated in a single-celled organism such as yeast is a first step in understanding the same processes in humans, which have a vastly larger and more complex genome," said Dr. Larson. "But fundamentally, the same molecular laws governing transcription factors will still apply."


'/>"/>

Contact: Kim Newman
sciencenews@einstein.yu.edu
718-430-3101
Albert Einstein College of Medicine
Source:Eurekalert

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/15/2016)... 2016 Research and Markets has ... Market 2016-2020,"  report to their offering.  , ... ,The global gait biometrics market is expected to ... period 2016-2020. Gait analysis generates multiple ... used to compute factors that are not or ...
(Date:3/31/2016)... , March 31, 2016  Genomics firm Nabsys ... founding CEO, Barrett Bready , M.D., who returned ... of the original technical leadership team, including Chief Technology ... of Product Development, Steve Nurnberg and Vice President of ... to the company. Dr. Bready served as ...
(Date:3/22/2016)... PROVO and SANDY, Utah ... Ontario (NSO), which operates the highest sample volume laboratory ... and Tute Genomics and UNIConnect, leaders in clinical sequencing ... announced the launch of a project to establish the ... panel. NSO has been contracted by ...
Breaking Biology News(10 mins):
(Date:5/27/2016)... At present, the Biotech sphere is in a ... volatility is what makes this industry interesting to consider. Here ... (NASDAQ: SNTA ), CTI BioPharma Corp. (NASDAQ: ... and Heat Biologics Inc. (NASDAQ: HTBX ). Sign ... these stocks at: http://www.activewallst.com/register/ ...
(Date:5/26/2016)... Despite the volatility that continues to envelop the ... research on ActiveWallSt.com directs the investor community,s focus on the ... ), Cerus Corp. (NASDAQ: CERS ), Arrowhead Pharmaceuticals ... Inc. (NASDAQ: FPRX ). Register with us today ... On Wednesday, shares in Massachusetts ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lajollacooks4u has become a rising hotspot for specialized ... of its top attractions. Fortune 500 companies, such as Illumina, Hewlett-Packard, Qualcomm and ... intimate team-building experience. , Each event kicks off with an olive oil and salt-tasting ...
(Date:5/25/2016)... ... May 25, 2016 , ... The ... by the Office of the National Coordinator for Health IT (ONC) outlining a ... clinically relevant data were available when and where it was needed. The organization ...
Breaking Biology Technology: