Navigation Links
Scientists learn structure of enzyme in unusual virus
Date:9/17/2007

WEST LAFAYETTE, Ind. -- Biologists have determined the three-dimensional structure of an unusual viral enzyme that is required in the assembly of new viruses.

The Paramecium bursaria chlorella virus infects a green alga called chlorella, transferring its DNA into host cells. Once inside the chlorella, the virus DNA makes an enzyme called glycosyltransferase, which is needed to produce structural proteins that are assembled to create the outer shells, or capsids, for new virus particles.

In contrast, many viruses commandeer the genes of host cells to make enzymes and proteins, said Ying Zhang, a postdoctoral researcher in the laboratory of Michael Rossmann, Purdue University's Hanley Distinguished Professor of Biological Sciences.

The three-dimensional structure of the complete infectious virus had been determined earlier by the same group of researchers and their colleagues. Now they have found the structure of a specific type of glycosyltransferase and also its complex with a molecule called UDP-glucose, which stands for uridine-5-diphosphate-glucose, along with positively charged manganese ions. The manganese ions are critical because they coordinate the binding of the UDP-glucose to the enzyme.

The findings are detailed in a research paper appearing in this month's issue of the journal Structure. The paper was written by Zhang; Purdue postdoctoral researcher Ye Xiang; James Van Etten, the William Allington Distinguished Professor of Plant Pathology at the University of Nebraska; and Rossmann.

Learning the fundamental mechanisms for how this glycosyltransferase works may later enable scientists to develop drugs that inhibit certain viral infections, Zhang said.

The glycosyltransferase apparently breaks a chemical bond between UDP and the glucose. The glucose is then attached to the roughly 5,000 copies of a protein that assembles to form the viral capsid that surrounds and protects the virus's DNA genome.

"The glucose may be helping to correctly fold the protein while it is being assembled into the capsid," Rossmann said.

In addition, the glucose on the capsid also may be involved in the initiation of the viral infection, he said.

The researchers used X-ray crystallography to determine the structure of the glycosyltransferase enzyme and earlier had used cryoelectron microscopy to determine the three-dimensional structure of the virus.


'/>"/>

Contact: Emil Venere
venere@purdue.edu
765-494-4709
Purdue University
Source:Eurekalert

Related biology news :

1. Wisconsin scientists grow critical nerve cells
2. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
3. UCSB scientists probe sea floor venting to gain understanding of early life on Earth
4. UAB scientists discover the origin of a mysterious physical force
5. Fox Chase Cancer Center scientists identify immune-system mutation
6. Scientists Replicate Hepatitis C Virus in Laboratory
7. Scientists detect probable genetic cause of some Parkinsons disease cases
8. Scientists find missing enzyme for tuberculosis iron scavenging pathway
9. Scientists seek answers on what activates deadly anthrax spores
10. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
11. Scientists collaborate to assess health of global environment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/26/2016)... LONDON , April 26, 2016 /PRNewswire/ ... Systems, a product subsidiary of Infosys (NYSE: ... partnership to integrate the Onegini mobile security platform ... http://photos.prnewswire.com/prnh/20151104/283829LOGO ) The integration ... security to access and transact across channels. Using ...
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
(Date:3/23/2016)... 23, 2016 ... Gesichts- und Stimmerkennung mit Passwörtern     ... MESG ), ein führender Anbieter digitaler ... mit SpeechPro zusammenarbeitet, um erstmals dessen Biometrietechnologie ... die Möglichkeit angeboten, im Rahmen mobiler Apps ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... ... 2016 , ... FireflySci has been manufacturing quartz and glass ... the globe. Their cute firefly logo has been spreading to more and more ... calibration standards that never require recalibration. These revolutionary standards have changed many ...
(Date:5/25/2016)... ... May 25, 2016 , ... The Ankle ... plating options designed to address fractures of the distal tibia and fibula. This ... Acumed Ankle Plating System 3 is composed of seven plate families that span ...
(Date:5/25/2016)... ... 25, 2016 , ... Scientists at the University of Athens say they have ... be hampering the research that could lead to one good one. Surviving Mesothelioma has ... now. , The team evaluated 98 mesothelioma patients who got a ...
(Date:5/25/2016)... ... May 25, 2016 , ... Biohaven Pharmaceutical Holding Company ... granted the company’s orphan drug designation request covering BHV-4157 for the treatment of ... the FDA. , Spinocerebellar ataxia is a rare, debilitating neurodegenerative disorder that ...
Breaking Biology Technology: