Navigation Links
Scientists engineer strain of MERS coronavirus for use in a vaccine
Date:9/9/2013

utated several of its genes, one by one, to study the effects on the virus' ability to infect, replicate, and re-infect cultured human cells.

Mutations that disabled accessory genes 3, 4a, 4b and 5 did not seem to hinder the virus: mutant viruses had similar growth rates as the wild-type virus, indicating that the mutations do not disable the virus enough to deploy the mutants in a vaccine. Mutations in the envelope protein (E protein), on the other hand, enabled the virus to replicate its genetic material, but prevented the virus from propagating, or infecting nearby cells.

A large amount of the rMERS-CoV-ΔE virus would be needed for a live attenuated MERS vaccine. A virus that can't propagate itself would be unable to grow the volume needed without help. Enjuanes says they provided the virus with a supplemental form E protein.

"To grow the virus, we create what are called 'packaging cells' that express the E protein missing in the virus. The gene to encode this protein is integrated in the cell chromosomes and will not mix with the viral genes. Therefore, in these cells, and only within them, the virus will grow by borrowing the E protein produced by the cell," says Enjuanes. "When the virus in administered to a person for vaccination, this person will not be able to provide the E protein to the defective virus," so the virus will die off after producing antigens to train the human immune system to fight a MERS-CoV infection.

Enjuanes says rMERS-CoV-ΔE is a very promising vaccine candidate, but more work remains before they can start clinical trials. He says the mutation in the E protein that prevents the virus from propagating represents one safe guard, but the US Food and Drug Administration requires that a recombinant live attenuated vaccine strains include at least three safe guards to ensure the virus doesn't revert easily back to its virulent form. His group is currently working on introducing other disabling
'/>"/>

Contact: Jim Sliwa
jsliwa@asmusa.org
202-942-9297
American Society for Microbiology
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Stanford scientists develop gene therapy approach to grow blood vessels in ischemic limbs
2. Queens scientists seek vaccine for Pseudomonas infection
3. Scientists produce eye structures from human blood-derived stem cells
4. American Society of Plant Biologists honors early career women scientists
5. Brandeis scientists win prestigious prize for circadian rhythms research
6. Scientists discover new method of proton transfer
7. Salk scientists open new window into how cancers override cellular growth controls
8. WileyChina.com - Now Featuring Bespoke Pages for China’s Life Scientists
9. Scientists win $2 million to study new pathway in development and maintenance of lymphoma
10. UGA scientists reveal genetic mutation depicted in van Goghs sunflower paintings
11. Genetic mutation depicted in van Goghs sunflower paintings revealed by scientists
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/21/2015)... BOSTON , May 21, 2015 ... scientific potential of music for health, today announced a ... (BerkleeICE) . The partnership will center on collaboration on ...  As a first step in the collaboration, The Sync ... course on "Music as Medicine" in the 2015-2016 academic ...
(Date:5/19/2015)... 2015 Research and ... of the  "Genetic Testing Market Outlook 2018" ... , ,A recent report, Genetic Testing Market ... the current and future genetic testing market. ... working principles and types are covered in ...
(Date:5/11/2015)... , May 11, 2015  Through a well-rounded UAS ... had a strong showing at AUVSI,s Unmanned 2015 ... representatives of Ohio,s UAS industry met ... abroad from all points along the UAS ecosystem. ... Coalition,s (DDC) Vice President for Aerospace Rich Knoll . ...
Breaking Biology News(10 mins):The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 2The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 3Global Genetic Testing Market Outlook 2018 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2
... your skin crawla bug that crawls onto your lips while ... skin, bites, then gorges on your blood. And if that,s ... on a potentially deadly disease. Now Jean-Paul Paluzzi, ... Mississauga, believes that manipulating physiology to prevent the insects from ...
... April 29 A small piece of RNA appears to ... fibrosis (IPF), according to lung disease researchers at the University ... first to examine microRNAs in the disease, is available online ... Medicine . MicroRNAs are short strands of genetic ...
... 2010) -- Using a rare metal that,s not utilized by ... could help unlock the identities of thousands of difficult-to-study proteins, ... diseases. The research was published online this week in ... "We have combined the chemical capabilities of rhodium with what ...
Cached Biology News:The kiss of death: Research targets lethal disease spread by insect that bites lips 2Pitt researchers discover big role for microRNA in lethal lung fibrosis 2Synthetic enzymes could help ID proteins 2
(Date:5/21/2015)... 21, 2015 As part of its ... (BDWMC), Sun Health Foundation funded a $3.25 million project ... pharmacy with the most up-to-date features and technology. , ... The pharmacy's 45 staff members will begin moving from ... in the coming days. , The current pharmacy ...
(Date:5/20/2015)... SAN FRANCISCO, Calif. , May 20, 2015 ... VCYT ) today presented preliminary data demonstrating the ... distinguish idiopathic pulmonary fibrosis (IPF) from other interstitial ... The findings suggest the classifier,s potential to help ... surgery to resolve ambiguity in IPF diagnosis – ...
(Date:5/20/2015)... (PRWEB) May 20, 2015 The global ... of 22.3% during the forecast period of 2015 to ... increasingly being used in drug discovery and development process ... market growth. In addition to this,heavy investments by key ... applications are also propelling market growth. Browse 113 market ...
(Date:5/20/2015)... 20, 2015  Cypher Genomics, Inc., a leading ... Celgene Corporation to apply Cypher,s Coral TM ... with patient response to innovative medicines. The information ... accelerate patient access to the right medicine at ... and development and potentially inform new companion diagnostics.  ...
Breaking Biology Technology:Sun Health Foundation Provides $3.25M to Expand, Relocate Banner Del E. Webb Medical Center Pharmacy 2Sun Health Foundation Provides $3.25M to Expand, Relocate Banner Del E. Webb Medical Center Pharmacy 3Veracyte Presents Preliminary Data Demonstrating Ability of Molecular Classifier to Improve Non-Surgical IPF Diagnosis Using Bronchoscopy Samples 2Veracyte Presents Preliminary Data Demonstrating Ability of Molecular Classifier to Improve Non-Surgical IPF Diagnosis Using Bronchoscopy Samples 3Veracyte Presents Preliminary Data Demonstrating Ability of Molecular Classifier to Improve Non-Surgical IPF Diagnosis Using Bronchoscopy Samples 4Veracyte Presents Preliminary Data Demonstrating Ability of Molecular Classifier to Improve Non-Surgical IPF Diagnosis Using Bronchoscopy Samples 5Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 2Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 3Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 4Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 5Cypher Genomics Collaborates with Celgene Corporation to Identify Novel Genomic Biomarkers 2
... Bottle , ... Concord, NH (PRWEB) July 25, 2008 -- These days, ... sipping water from a SIGG bottle. "Carrying a SIGG bottle has become ... green," said Debby de Moulpied, founder and owner of Real Green Goods, ...
... Product Sales Driven by Robust MRSA Growth, SUNNYVALE, ... for the second quarter of 2008 of $42.1,million, an ... 2007. Net,loss was $7.5 million, or $(0.13) per share, ... loss of $5.2 million, or $(0.10) per share, in ...
... ROCM ) will announce quarterly results after,financial markets ... then,hold a quarterly conference call to discuss its earnings ... time (5:00 p.m. eastern time)., This call is ... Medical,s website at http://www.rocm.com . To listen live ...
Cached Biology Technology:SIGG Water Bottles: Hip But Not Necessarily Green; Were Madonna and Cindy Crawford Hoodwinked? 2Cepheid Reports Second Quarter Revenue of $42.1 Million 2Cepheid Reports Second Quarter Revenue of $42.1 Million 3Cepheid Reports Second Quarter Revenue of $42.1 Million 4Cepheid Reports Second Quarter Revenue of $42.1 Million 5Cepheid Reports Second Quarter Revenue of $42.1 Million 6Cepheid Reports Second Quarter Revenue of $42.1 Million 7Cepheid Reports Second Quarter Revenue of $42.1 Million 8Cepheid Reports Second Quarter Revenue of $42.1 Million 9Cepheid Reports Second Quarter Revenue of $42.1 Million 10Cepheid Reports Second Quarter Revenue of $42.1 Million 11Cepheid Reports Second Quarter Revenue of $42.1 Million 12Cepheid Reports Second Quarter Revenue of $42.1 Million 13Rochester Medical Announces Third Quarter 2008 Earnings Conference Call July 31, 2008 2
... to qualitatively detect EGFR ... paraffin-embedded (FFPE) tissue sections, ... cell preparations, using Chromogenic ... Fluorescent In Situ Hybridization ...
Request Info...
Many mammalian cell types and experimental situations create challenges for standard transfection experiments. The ViraPower Lentiviral Expression System overcomes these challenges with efficient vir...
...
Biology Products: