Navigation Links
Scientists discover structure of protein essential for quality control, nerve function
Date:1/14/2013

-control manager for the cellular protein-making factories called ribosomes. Occasionally a ribosome receives miscoded genetic instructions and produces certain types of abnormal proteins, known as "nonstop proteins" jamming the ribosomal machinery like a wrinkled sheet of paper in an office printer. Bengtson and Joazeiro found that Ltn1 fixes jammed ribosomes by tagging nonstop proteins with ubiquitin molecules, thereby marking them for quick destruction by roving cellular garbage-disposers called proteasomes.

"The question for us then was, "How does Ltn1 do this?' " said Joazeiro.

Pushing the Boundaries of Electron Microscopy

To help find out, he began a collaboration with Carragher and Potter, who run the National Resource for Automated Molecular Microscopy (NRAMM), an advanced electron microscope facility at TSRI that is funded by the National Institutes of Health's National Center for Research Resources.

Ltn1 was deemed too large for its structure to be determined by current nuclear magnetic resonance (NMR) technology, and, as the scientists know now, too flexible to allow the highly regular crystalline packing needed by X-ray crystallographers. "It's a very floppy molecule, so it would be hard to crystallize," said Potter.

Advanced electron microscopy offered a way, however. Dmitry Lyumkis, a graduate student in the NRAMM laboratory and first author of the study, took high-resolution images of yeast Ltn1 with an electron microscope. He then used sophisticated image and data processing software to align and average individual images. The technique eliminates much of the random "noise" that obscures single images and produces a sharp 3D picture of the protein.

No one has ever used electron microscopy to distinguish so manymore than 20conformations of such a small protein. "Usually electron microscopists determine no more than two or three conformational states, and they work with protein complexes w
'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. MBL scientists find bipolar marine bacteria, refuting everything is everywhere idea
2. 2 climate scientists win 2012 Vetlesen Prize for work on ozone hole, ice cores
3. Scientists reassemble the backbone of life with a particle accelerator
4. Scientists reassemble the backbone of life with a particle acceleratorynchrotron X-rays
5. Scripps Florida scientists uncover potential drug target to block cell death in Parkinsons disease
6. After decades of research, scientists unlock how insulin interacts with cells
7. Scientists use marine robots to detect endangered whales
8. Genome scientists launch Microbiome journal
9. Scientists mimic fireflies to make brighter LEDs
10. Scientists pinpoint molecular signals that make some women prone to miscarriage
11. Scientists discover that for Australia the long-beaked echidna may not be a thing of the past
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists discover structure of protein essential for quality control, nerve function
(Date:7/22/2014)... Freezing and thawing might not be good for the ... each fall as they prepare to survive Alaska,s winter ... and thawing outside than a steak does in your ... the spring in better shape than the steak," said ... lead author on a recent paper demonstrating that freeze ...
(Date:7/22/2014)... for Health Policy and Economics have linked tighter ... rates for schizophrenic individuals. , The study comes ... health actually save money, when other costs are ... an extra approval step before tests or treatments ... called prior authorization is intended to encourage ...
(Date:7/22/2014)... light with uncanny precision are key parts of ... manipulate light instead of electricity. But producing these ... with a precise beam of electrons, is often ... Princeton and Columbia universities have proposed a new ... grow these specialized materials, known as photonic crystals, ...
Breaking Biology News(10 mins):Alaska frogs reach record lows in extreme temperature survival 2Alaska frogs reach record lows in extreme temperature survival 3Are state Medicaid policies sentencing people with mental illnesses to prison? 2Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3
... federal state of Saxony-Anhalt most often suffers from ... by Brandenburg. Hans Hauner of Munich Technical University ... the regional differences in prevalence in general medical ... edition of Deutsches rzteblatt International ( ...
... critical of its life. Having run the gauntlet of air and ... must also evade hungry fish patrolling the beaches in its bid ... cays the risks are high: as many as 30% perish as ... headlong dash through the waves cost the intrepid hatchlings? Curious to ...
... solution to a sticky scientific problem in basic fluid ... even rate, but instead forms what look like fingers ... rivulets "gravity fingers," and the explanation for their formation ... wateror any liquidmeets the soil (or other medium). Knowing ...
Cached Biology News:Cost of hatchling turtles' dash for freedom 2MIT researchers explain mystery of gravity fingers 2
(Date:7/22/2014)... July 22, 2014 OHAUS Corporation, ... and mechanical balances and scales, proudly announces its ... won Laboratory Equipment magazine’s 2014 “Readers' Choice” Award ... consecutive year the Explorer was voted the best ... Readers’ Choice Awards celebrates “excellence in product design ...
(Date:7/22/2014)... SHENZHEN, China , July 22, ... (BGI Tech), a subsidiary of BGI, the world,s ... of a new human whole exome sequencing service ... offered service includes in-depth bioinformatics analysis and SNP ... December 31, 2014. Complete Genomics, ...
(Date:7/22/2014)... Quorum Review IRB, the industry leader in central ... called Quick Step™ . The offering went live on ... industry’s need for seamless study start-up with a large volume ... planning 50 or more North American investigator sites, as well ... count of 80 or more. Qualifying studies may also enroll ...
(Date:7/22/2014)... Sunnyvale, CA (PRWEB) July 22, 2014 ... begin on September 9th, 2014 and will comprise of ... and Application’ classes. The TechTrainings is a series of ... professionals, designed to help them reach the next step ... SAS class is taught by Jennifer Kang, Senior ...
Breaking Biology Technology:OHAUS Explorer Voted “Best Balance” in Laboratory Equipment’s 2014 Readers’ Choice Awards 2BGI Tech Launches Human Whole Exome Sequencing Service on Complete Genomics' Advanced Platform 2BGI Tech Launches Human Whole Exome Sequencing Service on Complete Genomics' Advanced Platform 3Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 2Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 3Clinovo TechTrainings: Next Session of SAS and CDISC Classes Start 9th September, 2014 2Clinovo TechTrainings: Next Session of SAS and CDISC Classes Start 9th September, 2014 3
... IRVINE, Calif., March 14 Masimo Corporation,(Nasdaq: MASI ), ... pulse oximetry, today announced that it is scheduled,to present at ... The Four Seasons Hotel in Las Vegas, NV on Wednesday, ... and CEO, and Mark P. de,Raad, Executive Vice President and ...
... FRANCISCO, Calif., March 14 ,Renovis, Inc. (Nasdaq: RNVS ... of drugs for major medical needs in the areas ... fourth quarter and year ended December 31, 2007., ... December 31, 2007 was,$1.3 million and $9.8 million, respectively, ...
... Quest Diagnostics,Incorporated (NYSE: DGX ), the ... announced that it is scheduled to speak,at the ... Conference on,Tuesday, March 18, 2008, at the Marriott ... scheduled to begin at,8:45 a.m. Eastern Time., ...
Cached Biology Technology:Masimo to Present at Citi Investment Research 5th Annual Small & Mid-Cap Conference 2Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 2Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 3Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 4Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 5Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 6Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 7Quest Diagnostics to Speak at Cowen and Company 28th Annual Health Care Conference 2
HSV-2 gD (0191)...
...
...
RayBio Human Angiogenesis Antibody Array 2.1 (8 array membranes) with Accessory, detects 23 angiogenic factors (for lysate) Class: Antibody Array Products Product Group: Antibody Array...
Biology Products: