Navigation Links
Scientists decipher structure of nature's 'light switch'
Date:5/31/2010

researchers and their collaborators have pieced together for the first time a detailed structure of a whole phytochrome.

Li and his collaborators studied a phytochrome from a common bacterium that is quite similar in biochemistry and function to those found in plants, but easier to isolate. Plant biologist Richard Vierstra of the University of Wisconsin provided the purified samples.

At Brookhaven, Li's group used two imaging techniques. First, they applied a layer of heavy metal dye to the purified phytochrome molecules to make them more visible, and viewed them using an electron microscope. This produced many two-dimensional images from a variety of angles to give the researchers a rough outline of the phytochrome map.

The scientists also froze the molecules in solution to produce another set of images that would be free of artifacts from the staining technique. For this set of images, the scientists used a cryo-electron microscope.

Using computers to average the data from each technique and then combine the information, the scientists were able to construct a three-dimensional map of the full phytochrome structure. The scientists then fitted the previously determined detailed structures of phytochrome fragments into their newly derived 3-D map to build an atomic model for the whole phytochrome.

Though the scientists knew the phytochrome was composed of two "sister" units, forming a dimer, the new structure revealed a surprisingly long twisted area of contact between the two individual units, with a good deal of flexibility at the untwisted ends. The structure supports the idea that the absorption of light somehow adjusts the strength or orientation of the contact, and through a series of conformation changes, transmits a signal down the length of the molecular interface. The scientists confirmed the proposed structural changes during photo-conversion by mutagenesis and biochemical assay.

The scientists studie
'/>"/>

Contact: Karen McNulty Walsh
kmcnulty@bnl.gov
631-344-8350
DOE/Brookhaven National Laboratory
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Scientists find genes associated with throat cancer through study of over 10,000 subjects
2. NIST scientists gain new core understanding of nanoparticles
3. Scientists conclude asteroid ended the age of dinosaurs
4. Scientists to study impact of gulf oil spill on marine food webs
5. Scripps Research scientists break barrier to creating potential therapeutic molecules
6. Singapore scientists exploit knowledge of p53 for increasing specificity of cancer treatments
7. FASEB Scientists Descend on Capitol Hill to Advocate for Increased Science Funding
8. Scientists make important step toward stopping plaque-like formations in Huntingtons disease
9. Scientists need to be more proactive, effective at public communication
10. ARS scientists in North Dakota help improve potato storage capabilities
11. Stanford scientists track polluted groundwater to the sea
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/21/2015)... 2015 Today, ZTE announced its Android smartphone ... as expected revenues in 2015 that relate to sales of FPC1025 ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, CEO ... manufacturer in China and we are proud ... 5 for Axon , its first ...
(Date:7/20/2015)... DENVER , July 20, 2015  Acuity ... Mobility Report: The Convergence of Commerce and Privacy" ... billion mobile biometric apps will be downloaded to ... users. The mobile biometrics market is projected to ... app revenue during the seven-year forecast period.    ...
(Date:7/9/2015)... Calif. , July 9, 2015  Synaptics Inc. (NASDAQ: ... solutions, announced today that it will report financial results ... Thursday, July 30, 2015 after the close of market. ... analysts and investors at 2:00 p.m. PT (5:00 p.m. ... To participate on the live call, analysts ...
Breaking Biology News(10 mins):FPC's Touch Fingerprint Sensor FPC1025 in ZTE's Smartphone Axon 2Biometrics on Smart Mobile Devices to Redefine Digital Identity with 12.9 Billion Biometric App Downloads between 2014 and 2020 2Synaptics to Report Fourth Quarter, Fiscal Year 2015 Results on July 30 2
... release is available in German . ... in Freiburg have gained important insights for stem cell research ... to the development of new treatments. As Rolf Kemler,s research ... determines the length of the telomeres and a signalling pathway ...
... , Akron, Ohio, June 27, 2012 Expertise in fluid ... a new research center designed to help physicians better diagnose ... neck and shoulders. Chiari malformation is a disorder of ... brain and spinal fluid. UA engineers are able to ...
... animal scientists are working together to meet the challenges of ... experimental Biology on 28th June will demonstrate innovative techniques and ... issues, crop yields and diminishing fish stocks. , In ... the world,s four most important crops has fallen short of ...
Cached Biology News:When does a headache need an engineer to fix it? 2When does a headache need an engineer to fix it? 3
(Date:7/23/2015)... ... 2015 , ... The health and wellness of brain tumor caregivers, often a ... tumor diagnosis. The American Brain Tumor Association presents interactive sessions that address shifting the ... July 24-25 in Chicago. , “We have understood for a long-time now that ...
(Date:7/23/2015)... , July 23, 2015 The Hong Kong ... given the prestigious accolade of "Most Innovative Technology ... world-class position in fostering innovation and technology development ... region.  The award has ... innovation and technology magazine and website with a ...
(Date:7/23/2015)... July 23, 2015 /PRNewswire/ - Portage Biotech Inc. ("Portage") ... Biohaven Pharmaceutical Holding Company Limited (Biohaven), are pleased ... New Drug (IND) Application with the U.S. Food ... of BHV-0223. Portage holds 54% equity interest in ... a glutamate modulating agent and Biohaven has entered ...
(Date:7/23/2015)... July 23, 2015 /PRNewswire/ – Sigma-Aldrich Corporation (NASDAQ: ... technology company, today announced the Company has received ISO ... Dekalb and Barton ... . The certification extends Sigma-Aldrich,s ability to manufacture ... and antibodies used in diagnostic assays, and provides in ...
Breaking Biology Technology:Reframing the Caregiver and Patient Mindset: Coping with the ‘New Normal’ 2Reframing the Caregiver and Patient Mindset: Coping with the ‘New Normal’ 3HKSTP Wins Most Innovative Technology Hub at The New Economy Awards 2015 2HKSTP Wins Most Innovative Technology Hub at The New Economy Awards 2015 3Portage's Biohaven Announces IND Filing for BHV-0223 for Phase 1 Clinical Testing 2Portage's Biohaven Announces IND Filing for BHV-0223 for Phase 1 Clinical Testing 3Portage's Biohaven Announces IND Filing for BHV-0223 for Phase 1 Clinical Testing 4Sigma-Aldrich Meets Evolving Quality and Regulatory Needs with ISO 13485 Expansion, Provides Diagnostic Customers with Reliable Critical Raw Materials 2Sigma-Aldrich Meets Evolving Quality and Regulatory Needs with ISO 13485 Expansion, Provides Diagnostic Customers with Reliable Critical Raw Materials 3
... 6 VIRxSYS Corporation announced today the publication ... Abundant Albumin Transcripts for Production of Therapeutic Proteins In ... The study describes a novel RNA therapy technique in which ... gene "hijacks" the albumin, causing it to produce the newly ...
... 6 Oxygen Biotherapeutics, Inc. (OTC Bulletin Board: OXBO) ... has posted a new entry on his blog on ... recent meetings in Zurich, Switzerland, further development plans for ... for clinical-grade Oxycyte, and plans for raising additional capital. ...
... April 6 /PRNewswire/ - Orcrist Bio Inc. (ORC) ... today the approval of its Phase I, open-label, ... volunteers, by the European Competent Authority in Seville, ... assess the safety, tolerability, pharmacokinetics and pharmacodynamics (induction ...
Cached Biology Technology:VIRxSYS Publishes New Study on RNA Therapy 2Orcrist Bio Inc. Receives Approval from European Competent Authority to Initiate its Planned Phase I, Open-label, Single-ascending-dose, Safety Study of HYC750 2
... siTrio siRNA --- a cocktail of ... gene of interestis our most popular product. ... than 75% knockdown of your target gene ... siRNA for transfection and confirmation of optimal ...
... offers a large collection of phage ... tissuea, cell lines, and mouse tissues. ... pHD9 vector. The size of cDNA ... from 200bp to 1000bp by gel ...
... A multi-purpose, high gel-strength agarose suitable for ... from conventional constant field to Pulsed Field ... gel strength and exclusion limits, Multi ABgarose ... reduced running times. This in turn means ...
7G10 anti-Fasciclin III...
Biology Products: