To create artificial human skin, human fibrin from plasma of healthy donors was used. Researchers then added tranexamic acid to prevent fibrinolysis, and calcium chloride to precipitate fibrin coagulation, and 0.1% aragose. These artificial-skin substitutes were grafted on the back of the nude mice, with the purpose of observing its evolution in vivo. The equivalent skin substitutes were analysed by transmission and scanning light and electron microscopy and inmunofluorescence.
The skin created in the laboratory showed adequate biocompatibility rates with the recipient and no rejection, dehiscence or infection was registered. Additionally, the skin of all animals used in the study started to show granulation after six days from implantation. Within the following twenty days, cicatrization was complete.
The experiment conducted by the University of Granada is the first to create artificial human skin with a dermis made of fibrin-agarose biomaterial. To this date, artificial skin substitutes were elaborated with other biomaterials as collagen, fibrin, polyglycolic acid, chitosan, etc.
These biomaterials "added resistance, firmness and elasticity to the skin" according to Prof. Jimnez Rodrguez. "Definitively, we have created a more stable skin with similar functionality to normal human skin."
|Contact: Jose Maria Jimenez Rodriguez|
University of Granada