Navigation Links
Scientists create 3-D models of whole mouse organs
Date:6/24/2010

New Haven, Conn.Yale University engineers have for the first time created 3D models of whole intact mouse organs, a feat they accomplished using fluorescence microscopy. The team reports its findings in the May/June issue of the Journal of Biomedical Optics, in a study published online this week.

Combining an imaging technique called multiphoton microscopy with "optical clearing," which uses a solution that renders tissue transparent, the researchers were able to scan mouse organs and create high-resolution images of the brain, small intestine, large intestine, kidney, lung and testicles. They then created 3D models of the complete organsa feat that, until now, was only possible by slicing the organs into thin sections or destroying them in the process, a disadvantage if more information about the sample is needed after the fact.

With traditional microscopy, researchers are only able to image tissues up to depths on the order of 300 microns, or about three times the thickness of a human hair. In that process, tissue samples are cut into thin slices, stained with dyes to highlight different structures and cell types, individually imaged, then stacked back together to create 3D models. The Yale team, by contrast, was able to avoid slicing or staining the organs by relying on natural fluorescence generated from the tissue itself.

When combined with optical clearing, multiphoton microscopyso called because it uses photons to excite naturally fluorescent cells within the tissuecan image a larger field-of-view at much greater depths and is limited only by the size of the lens used. Once the tissue is cleared using a standard solution that makes it virtually transparent to optical light, the researchers shine different wavelengths of light on it to excite the inherently fluorescent tissue. The fluorescence is displayed as different colors that highlight the different structures and tissue types (in the lung, for example, collagen is depicted as green while elastin shows up as red).

"The intrinsic fluorescence is just as effective as conventional staining techniques," said Michael Levene, associate professor at the Yale School of Engineering & Applied Science and the team leader. "It's like creating a virtual 3D biopsy that can be manipulated at will. And you have the added benefit that the tissue remains intact even after it's been imaged."

The Yale team was able to reach depths in excess of two millimetersdeep enough to image complete mouse organs. Typical tissue samples taken during patient biopsies are about this size as well, meaning the new technique could be used to create 3D models of biopsies, Levene said. This could be especially useful in tissues where the direction of a cancerous growth may make it difficult to know how to slice tissue sample, he noted.

In addition, the technology could eventually be used to trace fluorescent proteins in the mouse brain and see where different genes are expressed, or to trace where drugs travel in the body using fluorescent tagging, for example.

"Fluorescence microscopy plays such a key role throughout biology and medicine," Leven said. "The range of applications of this technique is immense, including everything from improved evaluation of patient tissue biopsies to fundamental studies of how the brain is wired."


'/>"/>

Contact: Suzanne Taylor Muzzin
suzanne.taylormuzzin@yale.edu
203-432-8555
Yale University
Source:Eurekalert  

Related biology news :

1. UK scientists working to help cut ID theft
2. Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes
3. Comet probes reveal evidence of origin of life, scientists claim
4. Scientists link fragile X tremor/ataxia syndrome to binding protein in RNA
5. Male elephants get photo IDs from scientists
6. Scientists retrace evolution with first atomic structure of an ancient protein
7. Muscle mass: Scientists identify novel mode of transcriptional regulation during myogenesis
8. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
9. Clemson scientists shed light on molecules in living cells
10. Scientists tackle mystery mountain illness
11. T. rex quicker than Becks, say scientists
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists create 3-D models of whole mouse organs
(Date:4/5/2017)... KEY FINDINGS The global market for stem ... 25.76% during the forecast period of 2017-2025. The rise ... growth of the stem cell market. Download ... The global stem cell market is segmented on the ... cell market of the product is segmented into adult ...
(Date:3/30/2017)... 2017  On April 6-7, 2017, Sequencing.com will host ... hackathon at Microsoft,s headquarters in Redmond, ... on developing health and wellness apps that provide a ... Genome is the first hackathon for personal genomics ... companies in the genomics, tech and health industries are ...
(Date:3/29/2017)... 2017  higi, the health IT company that operates ... America , today announced a Series B investment ... EveryMove. The new investment and acquisition accelerates higi,s strategy ... transform population health activities through the collection and workflow ... higi collects and secures data today on behalf of ...
Breaking Biology News(10 mins):
(Date:4/27/2017)... ... April 27, 2017 , ... Volunteers supported ... scientists, researchers, engineers, and industry professionals in visiting U.S. Congressional offices in Washington, ... in the world photonics industry. , This year, National Photonics Initiative ...
(Date:4/26/2017)... ... April 26, 2017 , ... Looking for gift ideas ... events company, offers one-of-a-kind gifts, ranging from gourmet cooking experiences to Farmer’s Market ... guests leave inspired with new cooking tips and techniques, thanks to Chef Jodi ...
(Date:4/26/2017)... ... April 26, 2017 , ... Baltimore bio tech firm, PathSensors, ... screening solution at the National Postal Forum 2017 in Baltimore, Maryland, May 21st ... accurate, easy to use and low cost threat detection solution for government and ...
(Date:4/25/2017)... (PRWEB) , ... April 25, 2017 , ... As part ... the series will explore the laboratory testing for DIC in order to illuminate this ... disorder which can occur in hospitalized patients resulting in a high degree of morbidity ...
Breaking Biology Technology: