MADISON, Wis. A grandfather clock is, on its surface, a simple yet elegant machine. Tall and stately, its job is to steadily tick away the time. But a look inside reveals a much more intricate dance of parts, from precisely-fitted gears to cable-embraced pulleys and bobbing levers.
Like exploring the inner workings of a clock, a team of University of Wisconsin-Madison researchers is digging into the inner workings of the tiny cellular machines called spliceosomes, which help make all of the proteins our bodies need to function. In a recent study published in the journal Nature Structural and Molecular Biology, UW-Madison's David Brow, Samuel Butcher and colleagues have captured images of this machine, revealing details never seen before.
In their study, they reveal parts of the spliceosome built from RNA and protein at a greater resolution than has ever been achieved, gaining valuable insight into how the complex works and also how old its parts may be.
By better understanding the normal processes that make our cells tick, this information could some day act as a blueprint for when things go wrong. Cells are the basic units of all the tissues in our bodies, from our hearts to our brains to our skin and lungs.
It may also help other scientists studying similar cellular machinery and, moreover, it provides a glimpse back in evolutionary time, showing a closer link between proteins and RNA, DNA's older cousin, than was once believed.
"It gives us a much better idea of how RNA and proteins interact than ever before," says Brow, a UW-Madison professor of biomolecular chemistry.
The spliceosome is composed of six complexes that work together to edit the raw messages that come from genes, cutting out (hence, splicing) unneeded parts of the message. Ultimately, these messages are translated into proteins, which do the work of cells. The team created crystals of a part of the spliceosome called U6, made
|Contact: David Brow|
University of Wisconsin-Madison