Navigation Links
Scientists announce human intestinal stem cell 'breakthrough' for regenerative medicine
Date:9/4/2011

Human colon stem cells have been identified and grown in a lab-plate for the first time. This achievement, made by researchers of the Colorectal Cancer Lab at the Institute for Research in Biomedicine (IRB Barcelona) and published in Nature Medicine, is a crucial advance towards regenerative medicine.

Throughout life, stem cells of the colon regenerate the inner layer of our large intestine in a weekly basis. For decades scientists had evidences of the existence of these cells yet their identity remained elusive. Scientists led by the ICREA Professor and researcher at the Institute for Research in Biomedicine (IRB Barcelona) Eduard Batlle discovered the precise localization of the stem cells in the human colon and worked out a method that allows their isolation and in vitro expansion, that is their propagation in lab-plates. Growing cells outside the body generally requires providing the cells in a lab-plate with the right mix of nutrients, growth factors and hormones. But in the same way that each of the more than 200 types of cells in our body differs from the others so too do optimal growing conditions in the lab. Consequently, human adult stem cell culture in labs has been a truly impossible mission until now. Batlle's team has also established the conditions for maintain living human colon stem cells (CoSCs) outside of the human body: "This is the first time that it has been possible to grow single CoSCs in lab-plates and to derive human intestinal stem cell lines in defined conditions in a lab setting," explains the IRB Barcelona researcher Peter Jung, first author of the study together with Toshiro Sato, from the University Medical Center Utrecht in The Netherlands.

The development, published by Batlle's research group in the prestigious journal Nature Medicine, arrives after more than 10 years of intense research focused on the characterization of the biology of the intestinal stem cells and its connection with cancer. The research has been made possible by close collaboration between Batlle's team and the group led by Hans Clevers at the Hubretcht Institute and University Medical Center Utrecht in The Netherlands, and Mara A. Blasco at the Spanish National Cancer Research Centre in Madrid (Spain). "For years, scientists all over the world have been trying to grow intestinal tissue in lab-plates; testing different conditions; using different nutritive media. But because the vast majority of cells in this tissue are in a differentiated state in which they do not proliferate, they survived only for a few days", explains Jung. "The aim of this study was to find a way to identify and select individual CoSCs and to grow them while maintaining their undifferentiated and proliferative state in lab conditions. Thus, we would be able to model how they grow in number and differentiate into normal intestinal epithelial cells in lab-plates", continues Jung. The scientific community now has a defined 'recipe' for isolating CoSCs and deriving stable CoSCs lines, which have the capacity to grow undifferentiated for months. In fact, "now we can maintain stem cells in a plate up to 5 months or we can induce these cells to differentiate artificially, as they do inside our bodies".

"This achievement opens up an exciting new area of research with the potential to bring about a huge breakthrough in regenerative medicine", says Jung. Regenerative medicine or the idea of repairing the body by developing new tissues and organs as the old ones wear out involves growing new cells from patients into tissues and organs in a lab. However, the main element for making regenerative medicine a reality, namely adult stem cells, are just starting to be understood. "Now that guidelines for growing and maintaining colon stem cells in the lab are in place, we have an ideal platform that could help the scientific community to determine the molecular bases of gastrointestinal cell proliferation and differentiation. It is also suspected that alterations in the biology of CoSCs are at origin of several diseases affecting the gastrointestinal tract, such as colorectal cancer or Crohn's disease, an autoimmune and inflammatory disorder. Our discovery also paves the way to start exploring this exciting field," finishes Jung.


'/>"/>

Contact: Itziar Castanedo
itziar.castanedo@irbbarcelona.org
34-934-031-301
Institute for Research in Biomedicine (IRB Barcelona)
Source:Eurekalert

Related biology news :

1. Scientists unravel the cause of rare genetic disease: Goldman-Favre Syndrome explained
2. Hurricane Irene: Scientists collect water quality and climate change data from huge storm
3. Tropical coral could be used to create novel sunscreens for human use, say scientists
4. Scientists find new drug candidates for set of protein-folding diseases
5. Weight loss without the hunger: Cornell scientists say eat a lighter lunch
6. Scientists receive grant to develop new DNA sequencing method
7. Cars could run on recycled newspaper, Tulane scientists say
8. Scientists create natural Alzheimers-fighting compound in lab
9. Scientists reengineer antibiotic to overcome dangerous antibiotic-resistant bacteria
10. Scripps Research scientists help pinpoint cause of stress-related DNA damage
11. Penn molecular scientists develop color-changing stress sensor
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/20/2017)... DAL ) customers now can use fingerprints instead of their boarding ... ... biometrics to board aircraft at Reagan Washington National Airport ... Delta,s biometric boarding pass experience that launched in May at the ... to allow eligible Delta SkyMiles Members who are enrolled in CLEAR to ...
(Date:6/14/2017)... , June 15, 2017  IBM (NYSE: IBM ) is ... tech event dedicated to developing collaboration between startups and global ... June 15-17. During the event, nine startups will showcase the ... in various industries. France ... international market, with a 30 percent increase in the number ...
(Date:5/16/2017)... , May 16, 2017   Bridge Patient ... organizations, and MD EMR Systems , an ... partner for GE, have established a partnership to ... product and the GE Centricity™ products, including Centricity ... These new integrations will allow ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... 11, 2017 , ... A new study published in Fertility ... fresh in vitro fertilization (IVF) transfer cycles. The multi-center matched cohort ... After comparing the results from the fresh and frozen transfer cohorts, the authors ...
(Date:10/10/2017)... , ... October 10, 2017 , ... ... Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives of the ... for Excellence in Volunteer Experience from US2020. , US2020’s mission is to change ...
(Date:10/10/2017)... (PRWEB) , ... October 10, 2017 , ... ... innovation and business process optimization firm for the life sciences and healthcare industries, ... conference in San Francisco. , The presentation, “Automating GxP Validation for Agile ...
(Date:10/9/2017)... ... October 09, 2017 , ... At its national board meeting in North ... in Harvard University’s Departments of Physics and Astronomy, has been selected for membership in ... winning team for the 2015 Breakthrough Prize in Fundamental physics for the discovery of ...
Breaking Biology Technology: