Navigation Links
Scientist IDs genes that promise to make biofuel production more efficient, economical
Date:8/19/2010

URBANA A University of Illinois metabolic engineer has taken the first step toward the more efficient and economical production of biofuels by developing a strain of yeast with increased alcohol tolerance.

Biofuels are produced through microbial fermentation of biomass crops, which yield the alcohol-based fuels ethanol and iso-butanol if yeast is used as the microbe to convert sugars from biomass into biofuels.

"However, at a certain concentration, the biofuels that are being created become toxic to the yeast used in making them. Our goal was to find a gene or genes that reduce this toxic effect," said Yong-Su Jin, an assistant professor of microbial genomics in the U of I Department of Food Science and Human Nutrition and a faculty member in the U of I's Institute for Genomic Biology.

Jin worked with Saccharomyces cerevisiae, the microbe most often used in making ethanol, to identify four genes (MSN2, DOG1, HAL1, and INO1) that improve tolerance to ethanol and iso-butanol when they are overexpressed.

"We expect these genes will serve as key components of a genetic toolbox for breeding yeast with high ethanol tolerance for efficient ethanol fermentation," he said.

To assess the overexpressed genes' contribution to the components that have limited biofuel production, the scientists tested them in the presence of high concentrations of glucose (10%), ethanol (5%), and iso-butanol (1%) and compared their performance to a control strain of S. cerevisiae.

Overexpression of any of the four genes remarkably increased ethanol tolerance, but the strain in which INO1 was overexpressed elicited the highest ethanol yield and productivitywith increases of more than 70 percent for ethanol volume and more than 340 percent for ethanol tolerance when compared to the control strain.

According to Jin, the functions of the identified genes are very diverse and unrelated, which suggests that tolerance to high concentrations of iso-butanol and ethanol might involve the complex interactions of many genetic elements in yeast.

"For example, some genes increase cellular viability at the expense of fermentation. Others are more balanced between these two functions," he said.

"Identification of these genes should enable us to produce transportation fuels from biomass more economically and efficiently. It's a first step in understanding the cellular reaction that currently limits the production process," he said.

Further study of these genes should increase alcohol tolerance even further, and that will translate into cost savings and greater efficiency during biofuel production, he added.


'/>"/>

Contact: Phyllis Picklesimer
p-pickle@illinois.edu
217-244-2827
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert

Related biology news :

1. Scientists find new twist on drug screening to treat common childhood cancer
2. Scientists uncover Achilles heel of chronic inflammatory pain
3. Scientists successfully use human induced pluripotent stem cells to treat Parkinsons in rodents
4. Scientists reveal new targets for anti-angiogenesis drugs
5. Scripps Research scientists uncover possible cocaine addiction trigger
6. Free statins with fast food could neutralize heart risk, scientists say
7. Scientists discover oldest evidence of human stone tool use and meat-eating
8. Scientists identify DNA that may contribute to each persons uniqueness
9. Common orchid gives scientists hope in face of climate change
10. Scientists show theres nothing boring about watching paint dry
11. The jellyfish-like salp: Most efficient filter-feeder in the deep, scientists discover
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/19/2016)... , UAE, April 20, 2016 ... be implemented as a compact web-based "all-in-one" system solution ... the biometric fingerprint reader or the door interface with ... of modern access control systems. The minimal dimensions of ... ID readers into the building installations offer considerable freedom ...
(Date:4/14/2016)... 14, 2016 BioCatch ™, ... today announced the appointment of Eyal Goldwerger ... Goldwerger,s leadership appointment comes at a time ... the deployment of its platform at several of the ... which discerns unique cognitive and physiological factors, is a ...
(Date:3/31/2016)... March 31, 2016  Genomics firm Nabsys has completed ... Barrett Bready , M.D., who returned to the ... original technical leadership team, including Chief Technology Officer, ... Development, Steve Nurnberg and Vice President of Software and ... company. Dr. Bready served as CEO of ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 23, 2016 , ... Mosio, a leader in clinical research ... Recruitment and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the ... tools, and strategies for clinical researchers. , “The landscape of how patients receive ...
(Date:6/23/2016)... 2016 Houston Methodist Willowbrook Hospital has ... Association to serve as their official health care ... Willowbrook will provide sponsorship support, athletic training services, ... coaches, volunteers, athletes and families. "We ... Association and to bring Houston Methodist quality services ...
(Date:6/23/2016)... ... June 23, 2016 , ... Supplyframe, the Industry Network for ... Design Lab . Located in Pasadena, Calif., the Design Lab’s mission is to ... are designed, built and brought to market. , The Design Lab is Supplyframe’s ...
(Date:6/23/2016)... LONDON , June 23, 2016 ... & Hematology Review, 2016;12(1):22-8 http://doi.org/10.17925/OHR.2016.12.01.22 ... Review , the peer-reviewed journal from touchONCOLOGY, ... the escalating cost of cancer care is placing ... a result of expensive biologic therapies. With the ...
Breaking Biology Technology: