Navigation Links
Scientist IDs genes that promise to make biofuel production more efficient, economical
Date:8/19/2010

URBANA A University of Illinois metabolic engineer has taken the first step toward the more efficient and economical production of biofuels by developing a strain of yeast with increased alcohol tolerance.

Biofuels are produced through microbial fermentation of biomass crops, which yield the alcohol-based fuels ethanol and iso-butanol if yeast is used as the microbe to convert sugars from biomass into biofuels.

"However, at a certain concentration, the biofuels that are being created become toxic to the yeast used in making them. Our goal was to find a gene or genes that reduce this toxic effect," said Yong-Su Jin, an assistant professor of microbial genomics in the U of I Department of Food Science and Human Nutrition and a faculty member in the U of I's Institute for Genomic Biology.

Jin worked with Saccharomyces cerevisiae, the microbe most often used in making ethanol, to identify four genes (MSN2, DOG1, HAL1, and INO1) that improve tolerance to ethanol and iso-butanol when they are overexpressed.

"We expect these genes will serve as key components of a genetic toolbox for breeding yeast with high ethanol tolerance for efficient ethanol fermentation," he said.

To assess the overexpressed genes' contribution to the components that have limited biofuel production, the scientists tested them in the presence of high concentrations of glucose (10%), ethanol (5%), and iso-butanol (1%) and compared their performance to a control strain of S. cerevisiae.

Overexpression of any of the four genes remarkably increased ethanol tolerance, but the strain in which INO1 was overexpressed elicited the highest ethanol yield and productivitywith increases of more than 70 percent for ethanol volume and more than 340 percent for ethanol tolerance when compared to the control strain.

According to Jin, the functions of the identified genes are very diverse and unrelated, which suggests that tolerance to high concentrations of iso-butanol and ethanol might involve the complex interactions of many genetic elements in yeast.

"For example, some genes increase cellular viability at the expense of fermentation. Others are more balanced between these two functions," he said.

"Identification of these genes should enable us to produce transportation fuels from biomass more economically and efficiently. It's a first step in understanding the cellular reaction that currently limits the production process," he said.

Further study of these genes should increase alcohol tolerance even further, and that will translate into cost savings and greater efficiency during biofuel production, he added.


'/>"/>

Contact: Phyllis Picklesimer
p-pickle@illinois.edu
217-244-2827
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert

Related biology news :

1. Scientists find new twist on drug screening to treat common childhood cancer
2. Scientists uncover Achilles heel of chronic inflammatory pain
3. Scientists successfully use human induced pluripotent stem cells to treat Parkinsons in rodents
4. Scientists reveal new targets for anti-angiogenesis drugs
5. Scripps Research scientists uncover possible cocaine addiction trigger
6. Free statins with fast food could neutralize heart risk, scientists say
7. Scientists discover oldest evidence of human stone tool use and meat-eating
8. Scientists identify DNA that may contribute to each persons uniqueness
9. Common orchid gives scientists hope in face of climate change
10. Scientists show theres nothing boring about watching paint dry
11. The jellyfish-like salp: Most efficient filter-feeder in the deep, scientists discover
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2016)... , April 13, 2016  IMPOWER physicians supporting ... are setting a new clinical standard in telehealth thanks ... By leveraging the higi platform, IMPOWER patients can routinely ... pulse and body mass index, and, when they opt ... and convenient visit to a local retail location at ...
(Date:3/23/2016)... , March 23, 2016 ... erhöhter Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender ... das Unternehmen mit SpeechPro zusammenarbeitet, um erstmals ... Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im Rahmen ...
(Date:3/18/2016)... 2016 --> --> ... Manned & Unmanned Vehicles, Physical infrastructure and Perimeter Surveillance & ... the border security market and the continuing migration crisis in ... Europe has led visiongain to publish this unique ... --> defence & security companies in the border ...
Breaking Biology News(10 mins):
(Date:4/27/2016)... ... April 27, 2016 , ... ... Clark has joined the company as an Expert Consultant. Mr. Clark was ... collaborations and managing the development of small molecule monographs based on analytical methods. ...
(Date:4/27/2016)... ... 2016 , ... Global Stem Cells Group CEO Benito Novas announced ... of GSCG affiliate Kimera Labs in Miami. , In 2004, Ross received his Ph.D. ... for hematologic disorders and the suppression of graft vs. host disease (GVHD) under UM ...
(Date:4/27/2016)... , April 27, 2016 ... report with specific focus on US, EU, ... , to the healthcare business intelligence collection ... Complete report on the Flow Cytometry ... and supported with 282 tables and figures is ...
(Date:4/26/2016)... CAMBRIDGE, Mass. (PRWEB) , ... April 26, 2016 , ... ... Herr has been selected as one of three finalists for the European Inventor ... the EPO’s annual innovation prize will be announced at a ceremony in Lisbon on ...
Breaking Biology Technology: