Scientists in Canada have overcome a key research hurdle to developing regenerative treatments for diabetes and liver disease with a technique to produce medically useful amounts of endoderm cells from human pluripotent stem cells. The research, published in Biotechnology and Bioengineering, can be transferred to other areas of stem cell research helping scientists to navigate the route to clinical use known as the 'valley of death'.
"One million people suffer from type 1 diabetes in the United States, while liver disease accounts for 45,000 deaths a year," said Dr Mark Ungrin from the University of Toronto. "This makes stem cells, and the potential for regenerative treatments, hugely interesting to scientists. Laboratory techniques can produce thousands, or even millions, of these cells, but generating them in the numbers and quality needed for medicine has long been a challenge."
The research focused on the process of using pluripotent stem cells (PSC) to generate endoderm cells, one of the three primary germ layers which form internal organs including the lungs, pancreas, and liver. The ability to differentiate, or transform, PSCs into endoderm cells is a vital step to developing regenerative treatments for these organs.
"In order to produce the amount of endoderm cells needed for treatments it is important to understand how cells behave in larger numbers, for example how many are lost during the differentiation process and if all the cells will differentiate into the desired types," said Ungrin.
The team stained cells with fluorescent dye and as the cells divided, the dye was shared equally between the divided cells. By measuring the fluorescence of cell populations at a later stage the team were able to work out the frequency of cell division, which allowed them to predict how many cells would be present in a population at any given time.
This technique allowed the team to detect cell inefficiencies and d
|Contact: Ben Norman|