Navigation Links
San Diego Zoo scientists establish cell cultures of endangered frog
Date:11/3/2011

San Diego Zoo Global Wildlife Conservancy scientists have taken a tremendous leap forward in banking viable amphibian cells. This was achieved through the first successful establishment of cell cultures from frozen biopsy specimens of the critically endangered Mississippi gopher frog. A method called "tissue piecing" and immediate freezing in liquid nitrogen allows field biologists to collect samples that can later be processed in a laboratory.

"With amphibians we have found that we can routinely obtain viable cells from a fresh biopsy, but they fail to thrive and divide, leaving us often unable to establish and freeze cell cultures," said Oliver Ryder, Ph.D., San Diego Zoo Institute for Conservation Research director of genetics. "The question then is, how are we to know if there are viable cells in a tissue-pieced amphibian biopsy when we cannot grow the cells from a fresh biopsy?"

Thanks to a breakthrough achieved at the Institute for Conservation Research, the Mississippi gopher frog case provides proof that endangered amphibian cells can be grown and cells frozen from fresh or tissue-pieced and frozen biopsies.

The tissue-piecing technique has been used for some time with numerous species. In mammals, for example, scientists can mince a skin biopsy, treat it with cryoprotectant and freeze it. Later the tissue pieces can be thawed in a lab to establish a cell culture. But this method had not been previously successful with endangered amphibians until now.

"We are very pleased to have demonstrated for a critically endangered species that we have the techniques necessary for establishing cell cultures under field conditions, when quick access to a lab is not feasible," said Ryder. "For species for which we have not been able to successfully establish cell cultures, but have banked tissue-pieced samples, we know now that we have saved viable cells. In the future we now have the opportunity to go back when we hope to have worked out methods
'/>"/>

Contact: Christina Simmons
csimmons@sandiegozoo.org
619-685-3291
Zoological Society of San Diego
Source:Eurekalert

Page: 1 2

Related biology news :

1. UC San Diego biologists discover genes that repair nerves after injury
2. Firewood movement leading cause of oak infestation in San Diego County
3. San Diego Zoo Global joins effort to conserve the Amazonian rain forest through Peru field station
4. UC San Diego computer scientists lauded for computer systems security, bioinformatics work
5. San Diego Supercomputer Center participates in first Census of Marine Life
6. DOE awards UC San Diego consortium $9 million for algal biofuels research
7. Wireless sensor startup wins UC San Diego $80K Entrepreneur Challenge
8. American Society for Microbiology to host 110th general meeting in San Diego
9. Stimulus grant of nearly $9 million to UC San Diego funds big study of young brains
10. Siebel Foundation awards top UC San Diego bioengineering graduate students
11. UC San Diego engineer provides insights to decades-old DNA squabble
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/15/2014)... Geneticist Fred Alt will be awarded the 44th Rosenstiel ... University for his pioneering research exploring the mechanisms of ... and cancer cells. Alt is the second alumnus to ... the Nobel Prize in 2003. , Alt is the ... Genetics at Harvard Medical School and an investigator at ...
(Date:10/14/2014)... ATHENS, Ohio (Oct. 14, 2014)—It,s been millions of years ... but a team led by Ohio University scientists is ... to model airflow through dinosaur snouts. The research has ... not only breathe but to enhance the sense of ... pretty ,nosy, animals," said Ohio University doctoral student Jason ...
(Date:10/14/2014)... 2014 – High doses of fish oil supplements, rich in ... type of irregular heartbeat in which the heart can beat ... the AFFORD trial led by the Montreal Heart Institute were ... Cardiology on October 7th. , For the trial, 337 ... randomly assigned to 4 grams of fish oil a day ...
Breaking Biology News(10 mins):Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 3
... will be meeting in Greenville, SC next week as ... of America (ESA) get together for their 88th annual ... Center, March 2-5, 2014. The meeting will feature ... an awards ceremony, and an insect photo salon. ...
... New research from Plymouth State University and the ... horseshoe crabs for biomedical purposes causes short-term changes in ... population decline in parts of the east coast. ... blue blood from almost half a million living horseshoe ...
... -- There are more than a trillion cells called neurons ... of these neurons contains millions of proteins that perform different ... networks of the brain still remains as a mystery that ... time, a group of scientists has been able to observe ...
Cached Biology News:Southern insect scientists meeting in Greenville, S.C. 2Biomedical bleeding affects horseshoe crab behavior 2Biomedical bleeding affects horseshoe crab behavior 3Watching how the brain works 2
(Date:10/22/2014)... Involution Studios , a healthcare software design ... Understanding Ebola . The goal of the ... follow informative tool for anyone wanting to know more ... , "As the news has spread in all directions ... not only a healthcare crisis with global impact, but ...
(Date:10/22/2014)... According to new market research report ... Integration, Deployment & Support), by Service Model (SaaS, ... Others (Hybrid & Community)) - Global Forecast to ... Cloud Professional Services Market into various sub-segments with ... also identifies the drivers and restraints for this ...
(Date:10/22/2014)... and HONG KONG , Oct. ... disease therapeutics enterprise, announced today that rare disease expert ... as vice president, research. Dr. McKew brings more than ... leadership positions at the National Institutes of Health, Wyeth ... by Wyeth). Dr. McKew will lead aTyr,s efforts to expand ...
(Date:10/20/2014)... ON , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: ... $1.7 million which brings Q3 orders to $5.8 million and provides ... control projects in North America and one ... continue to bid new projects at record levels," said Peter ... were higher at the end of Q3 than they have been ...
Breaking Biology Technology:Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... Use in any standard fixed-angle ... 16,000 g.,Spin- centrifuge tube filters ... within a centrifuge tube. They filter ... removal, HPLC sample preparation, removal of ...
... in any standard fixed-angle microcentrifuge rotor ... g.,Spin- centrifuge tube filters consist of ... centrifuge tube. They filter by centrifugation ... sample preparation, removal of cells from ...
HybriWell seals securely to a microscope slide surface in seconds to enclose single or multiple specimens in a small reagent volume and eliminate evaporation....
... depth • Reusable, press-to-seal gasket and cover ... and disassembled for hybridizing and imaging thick ... with up to a 13 mm diameter. ... Minimize specimen handling, eliminate evaporation and leaking ...
Biology Products: