Navigation Links
Samurai sword protein makes strategic cuts in cell skeletons
Date:10/24/2013

through simulation that these branches would disorder the array unless some kind of pruning or culling mechanism was also in play.

Meanwhile, scientists at the University of Manchester in England had observed that misaligned microtubules were severed at junctions where a growing microtubule crosses an existing microtubule.

Zhang in the Dixit lab began with these observations and devised experiments to clarify the molecular mechanisms underlying them. He showed that they are insensitive to the geometry of the crossover or the stoutness of the underlying microtubule bundle. Instead, all that mattered was time.

How rapidly crossovers were cut determined what sort of array formed. In the transverse arrays, microtubules were cut, on average, within 41 seconds of a crossover event. In net-like arrays, on the other hand, cutting was three times slower and not as tightly controlled.

Plants have several severing proteins that might cut microtubules, but of these, katanin was the likeliest suspect. And in the katanin mutant, none of the crossovers were severed, demonstrating that this enzyme is solely responsible for cytoskeleton patterning.

To make sure, the scientists tagged katanin green and the microtubules red. When the cell was color coded in this way, they were able to see that katanin almost always localized to crossover sites before they were severed.

Coming in from the cold

These experiments showed that katanin was responsible for maintaining array patterning, but does it also create the pattern in the first place? To find out, Zhang used a trick he found in the literature, Dixit says.

Microtubules are cold sensitive and fall apart when they are chilled. "So Quan would put cells in the freezer for four or five minutes, take a slide out, run to the microscope, and watch to see what happened as the cells warmed up," Dixit says.

In the wild-type cells, the microtubules quickly re-ap
'/>"/>

Contact: Diana Lutz
dlutz@wustl.edu
314-935-5272
Washington University in St. Louis
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Global warming may have severe consequences for rare Haleakalā silverswords
2. Global warming may have severe consequences for rare Haleakalā silverswords
3. Iris Biometrics Leader EyeLock Joins the FIDO Alliance to Help Identify More Secure Replacement for Usernames and Passwords
4. IU biologists offer clearer picture of how protein machine systems tweak gene expression
5. Making memories: How 1 protein does it
6. Embryonic development protein active in cancer growth
7. More effective method of imaging proteins
8. The loss of a protein makes jump the tumor to the lymph node
9. Gold nanoantennas detect proteins
10. The Japanese traditional therapy, honokiol, blocks key protein in inflammatory brain damage
11. New hope for treating Alzheimers Disease: A role for the FKBP52 protein
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Samurai sword protein makes strategic cuts in cell skeletons
(Date:4/2/2015)... Md. , April 2, 2015 At ... Salt Lake City , the American College ... of five new directors to its Board.  Members of ... the ACMG and for forming and advancing its policies ... medical genetics profession. "It,s an eventful time ...
(Date:4/2/2015)... 2015 Fingerprint Cards (FPC) has ... from the distributor World Peace Industrial Group (WPI), part ... although the major part of the shipments will take ... by smartphone manufacturers in China . ... the communicated revenue guidance of + 1 000 MSEK for ...
(Date:4/1/2015)... Medisafe ™, the leading global medication management platform with ... Android smartphones and tablets, announces today that ... allow patients to track and correlate their adherence directly ... be able to visualize in real-time how taking medication ... glucose levels and blood pressure. "Patients need ...
Breaking Biology News(10 mins):American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 110 Million 2Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2
... living on the Port Valdez seafloor to stabilize after ... at the University of Alaska Fairbanks. "The earthquake, ... tsunami waves that followed, impacted every marine community in ... professor at the UAF School of Fisheries and Ocean ...
... YORK (April 24, 2011) -- A team of scientists ... Weill Cornell Medical College has shed light on the ... the cell membranes of neurons that modulate the transfer ... The research, published today in the journal Nature, ...
... ,Chicago, IL Stephen Maturo, MD, of Boston, MA, ... Award for outstanding achievement in the field of pediatric ... (EMG) in Children with Vocal Fold Mobility: Results of ... 29 May 1, 2011, in Chicago, IL. The ...
Cached Biology News:Port Valdez invertebrates stabilized 26 years after quake 2Molecular movements of neural transporters unveiled 2Molecular movements of neural transporters unveiled 3Molecular movements of neural transporters unveiled 4Molecular movements of neural transporters unveiled 5Stephen Maturo, M.D., ear, nose and throat specialist, receives Charles Ferguson Award 2
(Date:4/16/2015)... , April 16, 2015 /PRNewswire/ - Portage Biotech ... Canadian Securities Exchange: PBT.U), is pleased to announce that ... Show on April 25, 2015 to be held in ... Gregory Bailey and Mr. James Mellon ... and an update on the key programs at its ...
(Date:4/16/2015)... 2015 A new infographic compiles useful ... research institutions to learn how Appleā€™s new framework can ... timing is ideal since Apple made Research Kit ... an open-source framework for developing medical research apps that ... for researchers to collect secure clinical data and communicate ...
(Date:4/16/2015)... 2015  EMD Serono today announced the US ... Germany , appointed Alise Reicin , ... Clinical Development.  Dr. Reicin brings extensive research and ... and Immunology. She is an accomplished pharmaceutical executive ... Vice President in various capacities across R&D at ...
(Date:4/16/2015)... April 16, 2015  Northwest Biotherapeutics, Inc. (NASDAQ: ... developing non-toxic DCVax® personalized immune therapies for cancer, ... the Loncar Cancer Immunotherapy Index (LCINDX), a professional ... the immunotherapy field within the biotechnology space.  ... top 25 companies" in the immunotherapy space, including ...
Breaking Biology Technology:Portage to Present at Master Investor 2015 in London, UK 2Portage to Present at Master Investor 2015 in London, UK 3Portage to Present at Master Investor 2015 in London, UK 4Infographic Illustrates Potential for Research Kit to Impact Clinical Research 2Infographic Illustrates Potential for Research Kit to Impact Clinical Research 3Alise Reicin Joins the Biopharmaceutical Business of Merck KGaA, Darmstadt, Germany as Head of Global Clinical Development 2Alise Reicin Joins the Biopharmaceutical Business of Merck KGaA, Darmstadt, Germany as Head of Global Clinical Development 3NW Bio Included In New Index Of The "Top 25 Cancer Immunotherapy Companies" 2NW Bio Included In New Index Of The "Top 25 Cancer Immunotherapy Companies" 3
... COLUMBIA, Md., Sept. 13 Martek Biosciences,Corporation (Nasdaq: ... presenting at the,upcoming UBS Global Life Sciences Conference ... September 24-27, 2007 at the Grand Hyatt in ... on Tuesday, September 25th at,3:00 p.m. Eastern Time. ...
... 13 Vet-Stem, Inc., the global leader,in regenerative ... to the Central Veterinary Research Laboratory (CVRL) of,Dubai ... the laboratory to,offer adipose- derived stem cell services ... ruler of Dubai and Prime Minister of the ...
... Together Thought Leaders,in Life Sciences and Clinical Diagnostics ... Netherlands, Sept. 13 Luminex,Corporation (Nasdaq: LMNX ... the agenda for Planet xMAP Europe 2007, the ... will be,held 2-3 October at the Berlage,s Exchange ...
Cached Biology Technology:Vet-Stem and Central Veterinary Research Labs of Dubai Sign Exclusive License 2Luminex Corporation Hosts the Fifth Annual Planet xMAP Europe 2007 Symposium 2Luminex Corporation Hosts the Fifth Annual Planet xMAP Europe 2007 Symposium 3
Prepared in distilled water....
... egg white. The extinction coefficient , E(1%) ... mM phosphate-buffered saline, pH 7.4, ... and 50% glycerol. Avidin (from egg white) is ... by molecular sieving and is ...
NM522 contains an F episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F, NM522 should be grown on minimal (M-9) medium....
... Origami host strains are K-12 derivatives ... reductase (trxB) and glutathione reductase (gor) genes, ... the cytoplasm. Studies have shown that expression ... than in another host even though overall ...
Biology Products: