Navigation Links
Samurai sword protein makes strategic cuts in cell skeletons
Date:10/24/2013

through simulation that these branches would disorder the array unless some kind of pruning or culling mechanism was also in play.

Meanwhile, scientists at the University of Manchester in England had observed that misaligned microtubules were severed at junctions where a growing microtubule crosses an existing microtubule.

Zhang in the Dixit lab began with these observations and devised experiments to clarify the molecular mechanisms underlying them. He showed that they are insensitive to the geometry of the crossover or the stoutness of the underlying microtubule bundle. Instead, all that mattered was time.

How rapidly crossovers were cut determined what sort of array formed. In the transverse arrays, microtubules were cut, on average, within 41 seconds of a crossover event. In net-like arrays, on the other hand, cutting was three times slower and not as tightly controlled.

Plants have several severing proteins that might cut microtubules, but of these, katanin was the likeliest suspect. And in the katanin mutant, none of the crossovers were severed, demonstrating that this enzyme is solely responsible for cytoskeleton patterning.

To make sure, the scientists tagged katanin green and the microtubules red. When the cell was color coded in this way, they were able to see that katanin almost always localized to crossover sites before they were severed.

Coming in from the cold

These experiments showed that katanin was responsible for maintaining array patterning, but does it also create the pattern in the first place? To find out, Zhang used a trick he found in the literature, Dixit says.

Microtubules are cold sensitive and fall apart when they are chilled. "So Quan would put cells in the freezer for four or five minutes, take a slide out, run to the microscope, and watch to see what happened as the cells warmed up," Dixit says.

In the wild-type cells, the microtubules quickly re-ap
'/>"/>

Contact: Diana Lutz
dlutz@wustl.edu
314-935-5272
Washington University in St. Louis
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Global warming may have severe consequences for rare Haleakalā silverswords
2. Global warming may have severe consequences for rare Haleakalā silverswords
3. Iris Biometrics Leader EyeLock Joins the FIDO Alliance to Help Identify More Secure Replacement for Usernames and Passwords
4. IU biologists offer clearer picture of how protein machine systems tweak gene expression
5. Making memories: How 1 protein does it
6. Embryonic development protein active in cancer growth
7. More effective method of imaging proteins
8. The loss of a protein makes jump the tumor to the lymph node
9. Gold nanoantennas detect proteins
10. The Japanese traditional therapy, honokiol, blocks key protein in inflammatory brain damage
11. New hope for treating Alzheimers Disease: A role for the FKBP52 protein
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Samurai sword protein makes strategic cuts in cell skeletons
(Date:8/20/2014)... John and Rebecca Moores Professor of Biology and Biochemistry at ... the International Society for the Study of the Origin of ... two from the U.S., one from France and one ... elected every three years, and 36 have been named since ... countries, the ISSOL includes researchers from disciplines as varied as ...
(Date:8/20/2014)... SALT LAKE CITY Researchers at Huntsman Cancer Institute (HCI) ... mutated forms of the gene that encodes BCR-ABL, the ... (CML). According to the American Cancer Society, nearly 6,000 ... , Drugs already in use, called tyrosine kinase inhibitors ... disease. They do not cure CML but control it ...
(Date:8/20/2014)... OTTAWA , Aug. 20, 2014 /CNW/ -  ... without warning and can quickly become life threatening for some ... As many as 1.2 million Canadians have food allergies and ... six per cent of children are estimated to have food ... range from mild skin irritations and hives to breathing difficulties ...
Breaking Biology News(10 mins):UH professor named fellow by International Astrobiology Society 2Blueprint for next generation of chronic myeloid leukemia treatment 2Information Update - Allergens and food safety at school - What's in your child's lunch? 2Information Update - Allergens and food safety at school - What's in your child's lunch? 3
... (March 9, 2009)Osteoplastya highly effective minimally invasive ... metastatic bone disease by injecting bone cement ... pain relief, often presenting individuals who are ... effect," according to researchers at the Society ...
... for strokes has taken a significant step forward today ... stroke-damaged brain tissue in rats. The team of ... Research Council (BBSRC) and led by Dr Mike Modo ... The work, carried out at the Institute of Psychiatry ...
... much less expensive means of making artemisinin, the most ... also help to extend the effectiveness of this drug. ... be done at far lower costs than extracting the ... tree, making microbial-based artemisinin a much cheaper but equally ...
Cached Biology News:Metastatic bone disease patients can walk in Lazarus' footsteps 2Metastatic bone disease patients can walk in Lazarus' footsteps 3Stem cells replace stroke-damaged tissue in rats 2Synthetic biology can help extend anti-malaria drug effectiveness 2Synthetic biology can help extend anti-malaria drug effectiveness 3Synthetic biology can help extend anti-malaria drug effectiveness 4
(Date:8/21/2014)... Boston, MA (PRWEB) August 21, 2014 ... collaboration company, has been recognized as one of ... 500 | 5000 magazine for the 2nd consecutive ... on its 33rd annual Inc. 500|5000, an exclusive ... list represents the most comprehensive look at the ...
(Date:8/20/2014)... history lessons often have it that American Indians largely ... chicken pox, influenza and tuberculosis brought to the New ... vary, about 20 million people lived in the Americas ... them were killed by European diseases., But new research ... University and Johannes Krause of the University of Tubingen ...
(Date:8/20/2014)... Aug. 20, 2014  Decision Resources Group finds that the vascular ... Russia , India and ... 2013, roughly equal to the size of the United ... market will experience much faster growth as a result of strong ... Other key findings from Decision Resources Group,s coverage of the ...
(Date:8/20/2014)... SAN JOSE, Calif. , Aug. 20, 2014 ... UCSD highlights the transformative collaboration between the school,s ... (IoT).  As part of the Intel® Software Academic ... between computer sciences and the human body.  ... The study exhibits the work of ...
Breaking Biology Technology:SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 2BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 3Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2
... June 28, 2011 GeneTex, a leading manufacturer of ... that they will be launching a full line of ... as a model organism for scientific research. Due to their ... a useful model for genetic studies. Zebrafish eggs are ...
... While the health benefits of oregano oil is widely known ... from Global Healing Center is one of the most potent ... why it,s gaining popularity as a health supplement. ... 80% Carvacrol, a compound that is known scientifically for its ...
... 27, 2011 Metro Atlanta is building its reputation ... of Emory University and Children,s Healthcare of Atlanta, two ... million pediatric research facility. "Atlanta has a ... to continued medical advances that will benefit patients not ...
Cached Biology Technology:GeneTex Antibody Company to Launch a Line of New Zebrafish Primary Antibodies 2Organic Oregano Oil Gaining Popularity in Natural Health Field, According to Global Healing Center 2MAC Members Grow Atlanta's Bioscience Reputation 2MAC Members Grow Atlanta's Bioscience Reputation 3
... Clone/PAD: ZMD.471. Immunogen: Synthetic peptide ... the mouse junctophilin-1. Specificity: Specific ... Mouse (positive controls: mouse brain and ... and skeletal muscle tissues). Applications: ...
Anti-human VEGFR-1/Flt-1, clone Flt-1/EWI, Monoclonal Antibody...
HSV 1 IgM...
... Printing Surface (3 glass slides coated with 100 /500/1000 ... wafer coated with 1000 Angstroms of Gold over TI ... (3 PDMS micropatterned stamps with series of 100 / ... or 200 um x 10 mm lines, 3 PDMS ...
Biology Products: